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# EcoSal Plus Instructions for Authors

## Scope

EcoSal Plus is the authoritative online review journal that publishes an ever-growing body of expert reviews covering virtually all aspects of E. coli, Salmonella, and other members of the family Enterobacteriaceae and their use as model microbes for biological explorations. This journal is intended primarily for the research community as a comprehensive and continuously updated archive of the entire corpus of knowledge about the enteric bacterial cell. Thoughtful reviews focus on the physiology, metabolism, genetics, pathogenesis, ecology, genomics, systems biology, and history of E. coli and its relatives. These provide the integrated background needed for most microbiology investigations and are essential reading for research scientists. Articles contain links to E. coli K12 genes on the EcoCyc database site and are available as downloadable PDF files. Images and tables are downloadable to PowerPoint files.

## Submission

Please submit to ASM Press online submission and peer review system at:
http://ecosalplus.msubmit.net

Detailed instructions for submitting a manuscript are provided at the site. First-time users must create an account. During submission, the corresponding author is required to transfer copyright of the work to the ASM Press on behalf of all authors.

Most EcoSal Plus articles are commissioned by the Editorial Board. If you wish to submit an unsolicited article, please first submit a summary (abstract) and outline of your intended article to mangelini@asmusa.org. The summary should state what sets your article apart from the content currently on EcoSal Plus and why your article is an important contribution to this journal.

EcoSal Plus has no page charges or submission fees. However, author-paid optional open access is available that includes public access upon publication and open access provided through NIH’s PubMed Central repository. A corresponding author who is an ASM member pays a $1000 fee for optional open access. For a nonmember corresponding author, the fee is$2000. A corresponding author who is not an ASM member may join ASM to obtain the member rate.

Published authors receive a 1-year gratis individual subscription to EcoSal Plus.

Special Note for Authors Updating an Article: Please provide a summary of "what's new" in the revised article in the abstract. Submit your revised article in Word using the "tracked changes" tool. Once the updated article is published, the new version will contain a link to the previous PDF file of the article.

## Editorial Policy

EcoSal Plus follows the recommendations of the International Committee of Medical Journal Editors (ICMJE) and the Committee for Publications Ethics (COPE) for guidance on policies and procedures related to publication ethics. This includes prior publication or republication, authorship and contributions, conflict of interest, plagiarism, digital image manipulation, editorial decisions, and peer review. Authors and editors are expected to adhere to the highest ethical standards. The complete editorial policy is available here.

## Manuscript Formatting

• Manuscripts must contain an abstract.

• Submit a Word document. Double-space all elements (including text, references, figure legends, tables, and table footnotes). Type each table on a separate page, with all elements double spaced. With rare exceptions, the data in tables should be arranged so that columns of like material read down, not across.

• Use 1-inch (2.5-cm) margins.

• Include all desired links, especially if you discuss K12 genes included in the EcoCyc database.

• Follow standard ASM style, as detailed in the instructions to authors of any ASM journal (www.journals.asm.org) for other style questions, such as nomenclature, designations, and abbreviations.

## Figures

• Submit all figures as digital images. We may request high-quality hard copies of figures upon acceptance of your manuscript if there are problems with the digital image files.

• Continuous-tone figures (gels, micrographs, photographs, etc.) should be high-quality photographic prints. Digital files must have a resolution of at least 300 dpi. TIFF (.tif), JPEG (.jpg), and EPS (.eps) files are preferred. Do not submit photos as PowerPoint or Word files. Note: illustrations downloaded from the Internet are not acceptable unless their resolution meets or exceeds the minimum specified in these guidelines; the resolution of most photos on the Internet is insufficient for printing.

• Line figures should be high-quality laser prints on a white background. Avoid the use of gray shading; differences in shades of gray will not reproduce clearly. Use black or white fills or fill patterns such as cross-hatching instead. Avoid the use of fine lines (such as hairline rules) and tiny lettering, and do not use wide variations in type size within a single figure. Digital line art files (TIFF, JPEG, or EPS) should have a resolution of at least 1,200 dpi. PowerPoint files are also acceptable for line art.

• Figures must be able to withstand significant reduction.

• Do not embed figures in text or PowerPoint files. Each figure must be submitted as a separate file.

• Submit color figures if color is needed for proper or improved interpretation of the data by the reader.

• Computer-generated images may be processed only minimally. Processing, e.g., changing contrast, brightness, or color balance, is acceptable only if applied to all parts of the image, as well as to the controls, equally, and descriptions of all such adjustments and the tools used (both hardware and software) must be provided in the manuscript. Unprocessed data and files must be retained by the authors and be provided to the editor on request.

## Reference Style

• References must be numbered in the order in which they are cited in the text.

• Cite references in the text by number within parentheses (not superscript).

• Journals References to journals (printed or online) must include all authors’ names (do not use “et al.”), year, article title, journal name, volume, and inclusive page numbers. An example of an acceptable journal citation (modified PubMed/NLM reference style) follows:

7. Chen Y, McAleer KL, Murrell JC. 2010. Monomethylamine as a nitrogen source for a nonmethylotrophic bacterium, Agrobacterium tumefaciens. Appl Environ Microbiol 76:4102-4104.

• Books References to chapters in books (printed or online) must include all authors (do not use “et al.”), year, chapter title, and page numbers as well as the names of all editors, the title of the book, and the publisher and its location. An example of an acceptable book citation (modified PubMed/NLM reference style) follows:
5. Brandt ME, Warnock DW. 2015. Taxonomy and classification of fungi, p 1935-1943. In Jorgensen JH, Pfaller MA, Carroll KC, Funke G, Landry ML, Richter SS, Warnock DW (ed), Manual of Clinical Microbiology, 11th ed. ASM Press, Washington, DC.

• Other types of references to list in the References section: in-press references, patents that have been granted, theses and dissertations, published conference proceedings, published meeting abstracts, laws, letters to the editor, editorials, company publications (except package inserts), and online documents having authority and permanence (see below).

• References to cite in the text: unpublished data (including manuscripts in preparation), articles submitted for publication, personal communications, unpublished conference presentations, package inserts, computer software, databases, patent applications and patents pending, and commercial or evolving web pages (see below) (including temporary material on authors’ websites).

• Online and website references: References available online should be listed in the References section only if they have authority and permanence, e.g., online journals or books and final government and other documents and reports. Provide full facts of publication, including title, author (if available), owner/sponsor of website, URL, and date of publication, most recent revision, or most recent access. Authors are responsible for accuracy of all URLs.

## Permissions and Credit Lines

You are responsible for obtaining permission from the copyright owner (usually the original publisher) and the original author to reproduce or modify figures and tables and to reproduce text (in whole or in part) from previous publications. You may request permission via Copyright Clearance Center via RightsLink (http://www.copyright.com) or request a permission form from the production editor (mangelini@asmusa.org) to use when requesting permission. Note that all permissions must obtain a specific set of rights detailed on permission form.

It is your responsibility to give proper credit in the text. The following is an example of a credit line: “Reprinted from the Journal of Biological Chemistry (10) with permission of the publisher.”

## Original Publication

Information published in EcoSal Plus constitutes original publication and precludes the subsequent publication of the same information as an original publication in any journal.

This is a required field