Chapter 10 : New Cultivation Strategies for Terrestrial Microorganisms

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Soils are populated by microbial cells from all three domains of life: , , and . Cultivation of soil bacteria that may display the characteristics of true soil K-strategists can now be carried out routinely, albeit with some effort, and future comparisons with the better-known r-strategists will reveal any underlying physiological and genetic bases for these ecological strategies in soil bacteria. This chapter reviews some of the factors that allow successful isolation of a wider phylogenetic representation of soil bacteria than has traditionally been thought to be possible. Addition of cAMP to media results in significant increase in the cultivation efficiency of marine and freshwater bacteria but did not have an effect on the overall culturability of soil bacteria. Microorganisms can interact in a positive, neutral, or negative manner, and denser inocula can increase the likelihood of these interactions, and so potentially increase or decrease culturability. Some soil bacteria isolated in liquid media grow as colonies on the surfaces of glass tubes in which they are cultured, and do not disperse into liquid culture, and soil myxobacteria often grow on the solid surfaces of culture vessels when grown in liquid culture. The size of the inoculum is an important consideration when attempting to isolate soil bacteria.

Citation: Janssen P. 2008. New Cultivation Strategies for Terrestrial Microorganisms, p 173-192. In Zengler K (ed), Accessing Uncultivated Microorganisms. ASM Press, Washington, DC. doi: 10.1128/9781555815509.ch10

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Amounts of carbon, nitrogen, phosphorus, and sulfur required for colony formation by bacteria on plates

Citation: Janssen P. 2008. New Cultivation Strategies for Terrestrial Microorganisms, p 173-192. In Zengler K (ed), Accessing Uncultivated Microorganisms. ASM Press, Washington, DC. doi: 10.1128/9781555815509.ch10

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