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Chapter 1 : Introduction to Pathogenomics

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Abstract:

This chapter provides an introduction to pathogenomics. Any bacterial genome-sequencing project comprises a number of steps and follows the 80:20 principle: that is, 80%, or more, of the work gets done in 20% of the time, while finishing the job consumes a disproportionate amount of effort. The first stage in any bacterial genome project is to choose one or more strains to sequence. The next stage in a genome-sequencing project is to grow enough bacterial cells to yield sufficient genomic DNA for the creation of a random shotgun library in . Shotgun sequencing continues until an acceptable coverage of the genome has been achieved. The final stage in obtaining a bacterial genome sequence is finishing, during which all gaps are closed and all ambiguities resolved. A number of interesting features can be identified rapidly: repetitive sequences and homopolymeric tracts; noncoding RNA genes (tRNAs, rRNAs, small regulatory RNAs); recently acquired DNA; leading and lagging strands and the origin of replication. An interesting sideline will be the metagenomics of the bacteriophage community, given both the numerical preponderance of phages in the biosphere and their well-established role in the evolution and dissemination of bacterial virulence factors.

Citation: Pallen M. 2007. Introduction to Pathogenomics, p 1-20. In Pallen M, Nelson K, Preston G (ed), Bacterial Pathogenomics. ASM Press, Washington, DC. doi: 10.1128/9781555815530.ch1

Key Concept Ranking

Bacterial Proteins
0.58920234
Bacteria and Archaea
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Type III Secretion System
0.41915566
Nuclear Magnetic Resonance Spectroscopy
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0.58920234
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Tables

Generic image for table
TABLE 1

Milestones in bacterial genomics

Citation: Pallen M. 2007. Introduction to Pathogenomics, p 1-20. In Pallen M, Nelson K, Preston G (ed), Bacterial Pathogenomics. ASM Press, Washington, DC. doi: 10.1128/9781555815530.ch1
Generic image for table
TABLE 2

Bacterial genome sequencing centers and other resources

Citation: Pallen M. 2007. Introduction to Pathogenomics, p 1-20. In Pallen M, Nelson K, Preston G (ed), Bacterial Pathogenomics. ASM Press, Washington, DC. doi: 10.1128/9781555815530.ch1
Generic image for table
TABLE 3

Recent applications of microarrays in bacterial pathogenomics

Citation: Pallen M. 2007. Introduction to Pathogenomics, p 1-20. In Pallen M, Nelson K, Preston G (ed), Bacterial Pathogenomics. ASM Press, Washington, DC. doi: 10.1128/9781555815530.ch1
Generic image for table
TABLE 4

Sequencing of multiple strains from the same species

Citation: Pallen M. 2007. Introduction to Pathogenomics, p 1-20. In Pallen M, Nelson K, Preston G (ed), Bacterial Pathogenomics. ASM Press, Washington, DC. doi: 10.1128/9781555815530.ch1

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