Chapter 4 : a Postgenomic View

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This chapter primarily focuses on the studies that have made use of the two published genome sequences. Potential silent cassettes were identified for and , suggesting that the proteins encoded by the expression loci of the genes can undergo antigenic variation by a similar mechanism to . Nitric oxide (NO) is released from macrophages in response to lipopolysaccharide (LPS), and it is an intermediate in the denitrification of nitrite to nitrous oxide. Host neutrophils and macrophages commonly use superoxide radicals to eradicate bacteria after invasion of the host. Radicals generate strand breaks in DNA and RNA and affect fatty acid structure in the cell membranes. Three penicillin-binding proteins (PBPs) were identified experimentally in the and were considered to be reasonably well established. The genes encoding the RTX-like proteins are scattered across the chromosome and are present in different allelic versions in different strains. One of the most extensively investigated regulatory proteins of the spp. is MtrR, which was first identified as a regulator of the expression of the MtrCDE and Far AB efflux pumps in . Through hybridization of chromosomal DNA, rather than cDNA, the presence of sequences within a strain of interest can be determined.

Citation: Snyder L, Jordan P, Saunders N. 2007. a Postgenomic View, p 90-119. In Pallen M, Nelson K, Preston G (ed), Bacterial Pathogenomics. ASM Press, Washington, DC. doi: 10.1128/9781555815530.ch4

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