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Chapter 15 : National Molecular Subtyping Network for Food-Borne Bacterial Disease Surveillance in the United States

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National Molecular Subtyping Network for Food-Borne Bacterial Disease Surveillance in the United States, Page 1 of 2

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Abstract:

PulseNet USA is a network of public health laboratories from all 50 states, federal food regulatory agency laboratories, and a few state agricultural laboratories in the United States dedicated to molecular surveillance of food-borne bacterial infections. Regional and national PulseNet networks inspired by PulseNet USA have been established in different parts of the world dedicated to molecular surveillance of food-borne infections. PulseNets work together in investigations of international outbreaks in building capacity for molecular surveillance all over the world and in the development and validation of new PulseNet methods to ensure that data generated in all participating networks are comparable. As species are a common cause of diarrheal illness, active case finding during an outbreak can inevitably turn up additional infections that may or may not be related to the outbreak. Multilocus sequencing typing (MLST) has recently emerged as the current method of choice for subtyping of Campylobacters. A new priority area for PulseNet USA is to identify the sources of sporadic food-borne infections. The use and the content of the PulseNet database is therefore different from the other PulseNet databases, which are primarily aimed at detection of disease clusters by pulsedfield gel electrophoresis (PFGE) . However, the introduction of multilocus variable number tandem repeat analysis and single nucleotide polymorphism analysis will open the door to reliable phylogenetic analysis and attribution analysis for the non- organisms in PulseNet tool.

Citation: Gerner-Smidt P, Stroika S, Fitzgerald C. 2008. National Molecular Subtyping Network for Food-Borne Bacterial Disease Surveillance in the United States, p 277-285. In Nachamkin I, Szymanski C, Blaser M (ed), , Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555815554.ch15

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Single Nucleotide Polymorphism Analysis
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Restriction Fragment Length Polymorphism
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Figures

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Figure 1.

Organizational map of PulseNet USA.

Citation: Gerner-Smidt P, Stroika S, Fitzgerald C. 2008. National Molecular Subtyping Network for Food-Borne Bacterial Disease Surveillance in the United States, p 277-285. In Nachamkin I, Szymanski C, Blaser M (ed), , Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555815554.ch15
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Image of Figure 2.
Figure 2.

PulseNet International networks.

Citation: Gerner-Smidt P, Stroika S, Fitzgerald C. 2008. National Molecular Subtyping Network for Food-Borne Bacterial Disease Surveillance in the United States, p 277-285. In Nachamkin I, Szymanski C, Blaser M (ed), , Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555815554.ch15
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Image of Figure 3.
Figure 3.

PFGE cluster analysis of outbreak strains from different outbreaks with indistinguishable I and I patterns.

Citation: Gerner-Smidt P, Stroika S, Fitzgerald C. 2008. National Molecular Subtyping Network for Food-Borne Bacterial Disease Surveillance in the United States, p 277-285. In Nachamkin I, Szymanski C, Blaser M (ed), , Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555815554.ch15
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References

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Tables

Generic image for table
Table 1.

Number of entries in the PulseNet national PFGE databases as of the end of 2006

Citation: Gerner-Smidt P, Stroika S, Fitzgerald C. 2008. National Molecular Subtyping Network for Food-Borne Bacterial Disease Surveillance in the United States, p 277-285. In Nachamkin I, Szymanski C, Blaser M (ed), , Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555815554.ch15

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