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Chapter 26 : -Linked Flagellar Glycosylation in

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-Linked Flagellar Glycosylation in , Page 1 of 2

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Abstract:

Protein glycosylation has long been recognized as an important posttranslational modification in eukaryotic systems and one that imparts unique and diverse biological functions to the respective proteins. Although there is a considerable gap in our knowledge on the process of -linked glycosylation in prokaryotes as a result of the significant glycan diversity among prokaryotes, the -linked flagellar glycosylation system of has received considerable attention and is one of the more detailed prokaryotic systems studied to date. The first evidence for posttranslational modification of flagellin came from VC167 flagellin by direct chemical analysis of purified flagellar peptides. Mapping of glycosylation sites of VC167 flagellin also confirmed a conservation in localization to the central region of the monomer. Comparative genomic analyses of isolates has revealed that the flagellar locus displays considerable genetic variability. The glycans on flagellin appear to play complex roles in the biology of . The role of glycan composition on flagellar filaments was examined in vivo. Flagellins from the Epsilonproteobacteria, including both and spp., are not recognized by TLR5 receptors. Significant progress has been made in defining at the molecular level the structural nature of the novel sialic acid-like nonulosonate sugars found to be decorating the flagellar filaments of , and it is clear that these types of studies will be integral to future work exploring the role of novel glycan moieties in biological interactions.

Citation: Logan S, Schoenhofen I, Guerry P. 2008. -Linked Flagellar Glycosylation in , p 471-481. In Nachamkin I, Szymanski C, Blaser M (ed), , Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555815554.ch26

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Proteolytic Cleavage
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Protein Folding
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Carbohydrate Biosynthesis
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Figures

Image of Figure 1.
Figure 1.

Localization of flagellar glycosylation sites to surface-exposed regions of each monomer within the assembled filament. (A) Structure of FliC from serovar Typhimurium with major domains labeled as previously described ( ). The Protein Data Bank accession code is 1UCU and is displayed in Protein Explorer. (B) Assignment map of flagellin 81-176. The primary amino acid sequence of FlaA is shown, and modified residues are highlighted. The sequences corresponding to ND0, ND1, D2, D3, CD1, and CD0 domains from the FliC structure are indicated for FlaA on the left side.

Citation: Logan S, Schoenhofen I, Guerry P. 2008. -Linked Flagellar Glycosylation in , p 471-481. In Nachamkin I, Szymanski C, Blaser M (ed), , Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555815554.ch26
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Image of Figure 2.
Figure 2.

Structures of glycans found on flagellins compared with that of sialic acid. (A) Sialic acid or 5-acetamido-3,5-dideoxy--β--nonulosonic acid (Neu5Ac). (B) Pseudaminic acid or 5,7-diacetamido-3,5,7,9-tetradeoxy--α--nonulosonic acid and derivatives. (C) Legionaminic acid or 5,7-diacetamido-3,5,7,9-tetradeoxy--β--nonulosonic acid and derivatives. Known R groups are shown for both (B) and (C), illustrating the diversity of functional groups observed for each. Sialic acid and legionaminic acid exhibit the same absolute configuration. Confirmation of α or β linkage of B and C to flagellin has yet to be determined.

Citation: Logan S, Schoenhofen I, Guerry P. 2008. -Linked Flagellar Glycosylation in , p 471-481. In Nachamkin I, Szymanski C, Blaser M (ed), , Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555815554.ch26
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Image of Figure 3.
Figure 3.

CMP-Pse pathway of The enzymes and biosynthetic intermediates of the CMP–pseudaminic acid pathway, in order, are PseB, NAD(P)-dependent dehydratase/epimerase; PseC, PLP-dependent aminotransferase; PseH, -acetyltransferase; PseG, UDP-sugar hydrolase; PseI, pseudaminic acid synthase; PseF, CMP-pseudaminic acid synthetase; and (I) UDP-GlcNAc; (II) UDP-2-acetamido-2,6-dideoxy-β--hexos-4-ulose; (III) UDP-4-amino-4,6-dideoxy-β--AltNAc; (IV) UDP-2,4-diacetamido-2,4,6-trideoxy-β--altropyranose; (V) 2,4-diacetamido-2,4,6-trideoxy--altropyranose; (VI) pseudaminic acid; (VII) CMP–pseudaminic acid. Pyranose rings are shown as their predominant chair conformation in solution determined from nuclear Overhauser effects (NOEs) and coupling constants. Schematic representations of PseB and PseC structures from are shown ( ).

Citation: Logan S, Schoenhofen I, Guerry P. 2008. -Linked Flagellar Glycosylation in , p 471-481. In Nachamkin I, Szymanski C, Blaser M (ed), , Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555815554.ch26
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Image of Figure 4.
Figure 4.

Adherence pattern of green fluorescent protein (GPF)-tagged 81-176 cells to INT407 cells. A microcolony of 81-176 tagged with GFP on INT407 cells in culture after 18-h incubation ( ). The image shows complex interactions of bacteria on the intestinal epithelial cells, and some of the bacteria are visible as apparent chains. Phase microscopy of the same field confirmed that the monolayer remained intact.

Citation: Logan S, Schoenhofen I, Guerry P. 2008. -Linked Flagellar Glycosylation in , p 471-481. In Nachamkin I, Szymanski C, Blaser M (ed), , Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555815554.ch26
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Image of Figure 5.
Figure 5.

Molecular models of Pse5Ac7Ac, Leg5Am7Ac, and Leg5AmNMe7Ac. The exocyclic chain (C7 to C9) and pendant groups at C5 and C7 are displayed as reduced van der Waals spheres to show the extent of their structural diversities. Coordinates are available at http://ibs-isb.nrc-cnrc.gc.ca/facilities/NMR/molecularmodeling-e.html. From .

Citation: Logan S, Schoenhofen I, Guerry P. 2008. -Linked Flagellar Glycosylation in , p 471-481. In Nachamkin I, Szymanski C, Blaser M (ed), , Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555815554.ch26
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Tables

Generic image for table
Table 1.

O-linked flagellar glycans

Citation: Logan S, Schoenhofen I, Guerry P. 2008. -Linked Flagellar Glycosylation in , p 471-481. In Nachamkin I, Szymanski C, Blaser M (ed), , Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555815554.ch26

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