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Chapter 28 : Capsular Polysaccharide

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Abstract:

This chapter provides a review of the biochemistry, genetics, and biological roles of capsular polysaccharides (CPSs). The chapter is limited to consideration of PS capsules that have been detected in and other closely related species such as . Differentiation between groups is not based on antigenic or structural differences of the polysaccharide (PS) chains, but on such features as the mechanisms of biosynthesis, assembly, genetic regulation, and sequence similarity. The first indirect evidence of CPS produced by was reported. The first one to be studied was the mutant of strain NCTC 11168. Importantly, the absence or presence of these modifying groups affects the stainability of the PSs with silver and/or its interaction with homologous antiserum, which may in part explain their previous lack of detection in some serotypes. Differentiation of CPS-producing strains could be based on the probes corresponding to the genes involved in particular biosynthetic pathways. It would also be interesting to study a role of phosphoramidate modification of CPS in colonization and interaction with the host immune system. Initial attempts to develop a vaccine against infection, particularly in chickens, have not been very successful. Initial studies in the development of a human vaccine have focused on the use of heat- and formalin-attenuated whole-cell vaccines. In another study, a live attenuated strain vectoring the PEB1 antigen of , which is implicated in colonization ability was evaluated as a vaccine against infection, but no protection was observed.

Citation: V. Karlyshev A, W. Wren B, P. Moran A. 2008. Capsular Polysaccharide, p 505-521. In Nachamkin I, Szymanski C, Blaser M (ed), , Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555815554.ch28
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Figures

Image of Figure 1.
Figure 1.

Organization of gene clusters involved in the biosynthesis of enterobacterial groups II and III and CPSs. Open block arrows, genes encoding ABC transporter; solid block arrows, other genes involved in CPS transport and assembly; thin arrow, direction of transcription of the genes in the internal biosynthetic region (region 2 genes according to ).

Citation: V. Karlyshev A, W. Wren B, P. Moran A. 2008. Capsular Polysaccharide, p 505-521. In Nachamkin I, Szymanski C, Blaser M (ed), , Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555815554.ch28
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Image of Figure 2.
Figure 2.

Gene cassettes involved in Hep biosynthesis and correlation between gene (open arrows) and the presence of a deoxy form of Hep in the CPS repeating unit. Strains RM1221 (HS:53) ( ), 81-176 (HS:23/36) ( ), and CG8486 (HS:4) ( ). Solid arrows, other Hep-related genes; white arrows, other genes.

Citation: V. Karlyshev A, W. Wren B, P. Moran A. 2008. Capsular Polysaccharide, p 505-521. In Nachamkin I, Szymanski C, Blaser M (ed), , Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555815554.ch28
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Image of Figure 3.
Figure 3.

CPS structures of . HS:1 ( ), HS:2, ( ), HS: 3 ( ), HS:6, ( ), HS:19 ( ), HS:23/36 ( ), and HS:53 ( ). Some side chain groups may be absent as a result of structural variation. Fru, fructose, Hep, heptose, Gal, galactose; Glc, glucose; GlcA, glucuronic acid; Gro, glycerol; Man, mannose; Rib, ribose; NGro, aminoglycerol; NEtn, ethanolamine; MeON, O-methyl phosphoramidate. Furanose and pyranose configurations of sugars are denoted by letters and , respectively.

Citation: V. Karlyshev A, W. Wren B, P. Moran A. 2008. Capsular Polysaccharide, p 505-521. In Nachamkin I, Szymanski C, Blaser M (ed), , Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555815554.ch28
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Image of Figure 4.
Figure 4.

(A) Structure of Alcian blue dye (http://en.wikipedia.org/wiki/Image:AlcianBlue.png#file). (B) Electrophoretic detection of a lipid-free form of CPS from strain G1(HS:2) released after hydrolysis with a mixture of phospholipases D and A2 ( ); lane 1, native CPS; lane 2, lipid-free form of CPS; lane 3, LPS of serovar Typhimurium; lane 4, protein size markers. CPS, capsular polysaccharide; CPS-PL, lipid-free form of CPS; LOS, lipooligosaccharide.

Citation: V. Karlyshev A, W. Wren B, P. Moran A. 2008. Capsular Polysaccharide, p 505-521. In Nachamkin I, Szymanski C, Blaser M (ed), , Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555815554.ch28
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Image of Figure 5.
Figure 5.

Mechanism of release of a lipid-free form of CPS by phospholipases. The structure of the CPS lipid anchor determined for strain 81-176 ( ) with predicted cleavage sites by phospholipases A2 and D (according to ). X, polysaccharide moiety.

Citation: V. Karlyshev A, W. Wren B, P. Moran A. 2008. Capsular Polysaccharide, p 505-521. In Nachamkin I, Szymanski C, Blaser M (ed), , Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555815554.ch28
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Image of Figure 6.
Figure 6.

Detection of capsule from G1 (HS:2) by electron microscopy ( ).

Citation: V. Karlyshev A, W. Wren B, P. Moran A. 2008. Capsular Polysaccharide, p 505-521. In Nachamkin I, Szymanski C, Blaser M (ed), , Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555815554.ch28
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References

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1. Ashgar, S. S.,, N. J. Oldfield,, K. G. Wooldridge,, M. A. Jones,, G. J. Irving,, D. P. Turner, and, D. A. Ala’Aldeen. 2007. CapA, an autotransporter protein of Campylobacter jejuni, mediates association with human epithelial cells and colonization of the chicken gut. J. Bacteriol. 189:18561865.
2. Aspinall, G. O.,, C. M. Lynch,, H. Pang,, R. T. Shaver, and, A. P. Moran. 1995. Chemical structures of the core region of Campylobacter jejuni O:3 lipopolysaccharide and an associated polysaccharide. Eur. J. Biochem. 231:570578.
3. Aspinall, G. O.,, A. G. McDonald, and, H. Pang. 1994a. Lipopolysaccharides of Campylobacter jejuni serotype O:19: structures of O antigen chains from the serostrain and two bacterial isolates from patients with the Guillain-Barré syndrome. Biochemistry 33:250255.
4. Aspinall, G. O.,, A. G. McDonald, and, H. Pang. 1992. Structures of the O chains from lipopolysaccharides of Campylobacter jejuni serotypes O:23 and O:36. Carbohydr. Res. 231:1330.
5. Aspinall, G. O.,, A. G. McDonald,, H. Pang,, L. A. Kurjanczyk, and, J. L. Penner. 1994b. Lipopolysaccharides of Campylobacter jejuni serotype O:19: structures of core oligosaccharide regions from the serostrain and two bacterial isolates from patients with the Guillain-Barré syndrome. Biochemistry 33:241249.
6. Axelsson-Olsson, D.,, J. Waldenstrom,, T. Broman,, B. Olsen, and, M. Holmberg. 2005. Protozoan Acanthamoeba polyphaga as a potential reservoir for Campylobacter jejuni. Appl. Environ. Microbiol. 71:987992.
7. Bachtiar, B. M.,, P. J. Coloe, and, B. N. Fry. 2007. Knockout mutagenesis of the kpsE gene of Campylobacter jejuni 81116 and its involvement in bacterium-host interactions. FEMS Immunol. Med. Microbiol. 49:149154.
8. Bacon, D. J.,, C. M. Szymanski,, D. H. Burr,, R. P. Silver,, R. A. Alm, and, P. Guerry. 2001. A phase-variable capsule is involved in virulence of Campylobacter jejuni 81-176. Mol. Microbiol. 40:769777.
9. Bernatchez, S.,, C. M. Szymanski,, N. Ishiyama,, J. Li,, H. C. Jarrell,, P. C. Lau,, A. M. Berghuis,, N. M. Young, and, W. W. Wakarchuk. 2005. A single bifunctional UDP-GlcNAc/Glc 4-epimerase supports the synthesis of three cell surface glycoconjugates in Campylobacter jejuni. J. Biol. Chem. 280:47924802.
10. Black, R. E.,, M. M. Levine,, M. L. Clements,, T. P. Hughes, and, M. J. Blaser. 1988. Experimental Campylobacter jejuni infection in humans. J. Infect. Dis. 157:472479.
11. Blaser, M. J.,, P. F. Smith,, J. E. Repine, and, K. A. Joiner. 1988. Pathogenesis of Campylobacter fetus infections. Failure of encapsulated Campylobacter fetus to bind C3b explains serum and phagocytosis resistance. J. Clin. Invest. 81:14341444.
12. Boer, P.,, J. A. Wagenaar,, R. P. Achterberg,, J. P. Putten,, L. M. Schouls, and, B. Duim. 2002. Generation of Campylobacter jejuni genetic diversity in vivo. Mol. Microbiol. 44:351359.
13. Burr, D. H.,, D. Rollins,, L. H. Lee,, D. L. Pattarini,, S. S. Walz,, J. H. Tian,, J. L. Pace,, A. L. Bourgeois, and, R. I. Walker. 2005. Prevention of disease in ferrets fed an inactivated whole cell Campylobacter jejuni vaccine. Vaccine 23:43154321.
14. Byrne, C. M.,, M. Clyne, and, B. Bourke. 2007. Campylobacter jejuni adhere to and invade chicken intestinal epithelial cells in vitro. Microbiology 153:561569.
15. Charland, N.,, J. Harel,, M. Kobisch,, S. Lacasse, and, M. Gottschalk. 1998. Streptococcus suis serotype 2 mutants deficient in capsular expression. Microbiology 144:325332.
16. Chart, H.,, D. Conway,, J. A. Frost, and, B. Rowe. 1996a. Outer membrane characteristics of Campylobacter jejuni grown in chickens. FEMS Microbiol. Lett. 145:469472.
17. Chart, H.,, J. A. Frost,, A. Oza,, R. Thwaites,, S. Gillanders, and, B. Rowe. 1996b. Heat-stable serotyping antigens expressed by strains of Campylobacter jejuni are probably capsular and not long-chain lipopolysaccharide. J. Appl. Bacteriol. 81:635640.
18. Chatzipanagiotou, S.,, K. Kilidireas,, G. Trimis,, C. Nicolaou,, M. Anagnostouli,, C. Athanassaki,, A. Giannoulia,, N. Legakis, and, S. Youroukos. 2003. Campylobacter jejuni O:19 serotype-associated Guillain-Barré syndrome in a child: the first case reported from Greece. Clin. Microbiol. Infect. 9:6972.
19. Corcoran, A. T.,, H. Annuk, and, A. P. Moran. 2006. The structure of the lipid anchor of Campylobacter jejuni polysaccharide. FEMS Microbiol. Lett. 257:228235.
20. Corcoran, A. T., and, A. P. Moran. 2007. Influence of growth conditions on diverse polysaccharide production by Campylobacter jejuni. FEMS Immunol. Med. Microbiol. 49:124132.
21. Coward, C.,, A. J. Grant,, C. Swift,, J. Philp,, R. Towler,, M. Heydarian,, J. A. Frost, and, D. J. Maskell. 2006. Phase-variable surface structures are required for infection of Campylobacter jejuni by bacteriophages. Appl. Environ. Microbiol. 72:46384647.
22. Davey, M. E., and, M. J. Duncan. 2006. Enhanced biofilm formation and loss of capsule synthesis: deletion of a putative glycosyltransferase in Porphyromonas gingivalis. J. Bacteriol. 188:55105523.
23. Ezzell, J. W., and, S. L. Welkos. 1999. The capsule of Bacillus anthracis: a review. J. Appl. Microbiol. 87:250255.
24. Fouts, D. E.,, E. F. Mongodin,, R. E. Mandrell,, W. G. Miller,, D. A. Rasko,, J. Ravel,, L. M. Brinkac,, R. T. DeBoy,, C. T. Parker,, S. C. Daugherty,, R. J. Dodson,, A. S. Durkin,, R. Madupu,, S. A. Sullivan,, J. U. Shetty,, M. A. Ayodeji,, A. Shvartsbeyn,, M. C. Schatz,, J. H. Badger,, C. M. Fraser, and, K. E. Nelson. 2005. Major structural differences and novel potential virulence mechanisms from the genomes of multiple Campylobacter species. PLoS Biol. 3:e15.
25. Fry, B. N.,, S. Feng,, Y. Y. Chen,, D. G. Newell,, P. J. Coloe, and, V. Korolik. 2000. The galE gene of Campylobacter jejuni is involved in lipopolysaccharide synthesis and virulence. Infect. Immun. 68:25942601.
26. Ghannoum, M. A. 2000. Potential role of phospholipases in virulence and fungal pathogenesis. Clin. Microbiol. Rev. 13:122143.
27. Gilbert, M.,, R. E. Mandrell,, C. T. Parker,, J. Li, and, E. Vinogradov. 2007. Structural analysis of the capsular polysaccharide from Campylobacter jejuni RM1221. Chembiochemistry 8:625631.
28. Grant, A. J.,, C. Coward,, M. A. Jones,, C. A. Woodall,, P. A. Barrow, and, D. J. Maskell. 2005. Signature-tagged transposon mutagenesis studies demonstrate the dynamic nature of cecal colonization of 2-week-old chickens by Campylobacter jejuni. Appl. Environ. Microbiol. 71:80318041.
29. Guerry, P.,, C. M. Szymanski,, M. M. Prendergast,, T. E. Hickey,, C. P. Ewing,, D. L. Pattarini, and, A. P. Moran. 2002. Phase variation of Campylobacter jejuni 81-176 lipooligosaccharide affects ganglioside mimicry and invasiveness in vitro. Infect. Immun. 70:787793.
30. Hanniffy, O. M.,, A. S. Shashkov,, A. P. Moran,, M. M. Prendergast,, S. N. Senchenkova,, Y. A. Knirel, and, A. S. Savage. 1999. Chemical structure of a polysaccharide from Campylobacter jejuni 176.83 (serotype O:41) containing only furanose sugars. Carbohydr. Res. 319:124132.
31. Hendley, J. O.,, K. R. Powell,, N. L. Salomonsky, and, R. R. Rodewald. 1981. Electron microscopy of the gonococcal capsule. J. Infect. Dis. 143:796802.
32. Hendrixson, D. R., and, V. J. DiRita. 2004. Identification of Campylobacter jejuni genes involved in commensal colonization of the chick gastrointestinal tract. Mol. Microbiol. 52:471484.
33. Istivan, T. S.,, P. J. Coloe,, B. N. Fry,, P. Ward, and, S. C. Smith. 2004. Characterization of a haemolytic phospholipase A2 activity in clinical isolates of Campylobacter concisus. J. Med. Microbiol. 53:483493.
34. Jann, K., and, B. Jann 1987 Polysaccharide antigens of Escherichia coli. Rev. Infect. Dis. 9(Suppl. 5):S517S526.
35. Jeurissen, S. H.,, E. M. Janse,, N. van Rooijen, and, E. Claassen. 1998. Inadequate anti-polysaccharide antibody responses in the chicken. Immunobiology 198:385395.
36. Joshua, G. W.,, C. Guthrie-Irons,, A. V. Karlyshev, and, B. W. Wren. 2006. Biofilm formation in Campylobacter jejuni. Microbiology 152:387396.
37. Jung, S. Y.,, A. Matin,, K. S. Kim, and, N. A. Khan. 2007. The capsule plays an important role in Escherichia coli K1 interactions with Acanthamoeba. Int. J. Parasitol. 37:417423.
38. Kalmokoff, M.,, P. Lanthier,, T. L. Tremblay,, M. Foss,, P. C. Lau,, G. Sanders,, J. Austin,, J. Kelly, and, C. M. Szymanski. 2006. Proteomic analysis of Campylobacter jejuni 11168 biofilms reveals a role for the motility complex in biofilm formation. J. Bacteriol. 188:43124320.
39. Kanipes, M. I.,, E. Papp-Szabo,, P. Guerry, and, M. A. Monteiro. 2006. Mutation of waaC, encoding heptosyltransferase I in Campylobacter jejuni 81-176, affects the structure of both lipopolysachharide and capsular carbohydrate. J. Bacteriol. 188:32733279.
40. Karlyshev, A. V.,, O. L. Champion,, C. Churcher,, J. R. Brisson,, H. C. Jarrell,, M. Gilbert,, D. Brochu,, F. St Michael,, J. Li,, W. W. Wakarchuk,, I. Goodhead,, M. Sanders,, K. Stevens,, B. White,, J. Parkhill,, B. W. Wren, and, C. M. Szymanski. 2005. Analysis of Campylobacter jejuni capsular loci reveals multiple mechanisms for the generation of structural diversity and the ability to form complex heptoses. Mol. Microbiol. 55:90103.
41. Karlyshev, A. V.,, E. E. Galyov,, O. Smirnov,, A. P. Guzayev,, V. M. Abramov, and, V. P. Zav’yalov. 1992. A new gene of the f1 operon of Y. pestis involved in the capsule biogenesis. FEBS Lett. 297:7780.
42. Karlyshev, A. V.,, D. Linton,, N. A. Gregson,, A. J. Lastovica, and, B. W. Wren. 2000. Genetic and biochemical evidence of a Campylobacter jejuni capsular polysaccharide that accounts for Penner serotype specificity. Mol. Microbiol. 35:529541.
43. Karlyshev, A. V.,, M. V. McCrossan, and, B. W. Wren. 2001. Demonstration of polysaccharide capsule in Campylobacter jejuni using electron microscopy. Infect. Immun. 69:59215924.
44. Karlyshev, A. V., and, B. W. Wren. 2001. Detection and initial characterization of novel capsular polysaccharide among diverse Campylobacter jejuni strains using Alcian blue dye. J. Clin. Microbiol. 39:279284.
45. Khoury, C. A., and, R. J. Meinersmann. 1995. A genetic hybrid of the Campylobacter jejuni flaA gene with LT-B of Escherichia coli and assessment of the efficacy of the hybrid protein as an oral chicken vaccine. Avian Res. 39:812820.
46. Kilcoyne, M.,, A. P. Moran,, A. S. Shashkov,, S. N. Senchenkova,, J. A. Ferris,, A. T. Corcoran, and, A. V. Savage. 2006. Molecular origin of two polysaccharides of Campylobacter jejuni 81116. FEMS Microbiol. Lett. 263:214222.
47. Komagamine, T., and, N. Yuki. 2006. Ganglioside mimicry as a cause of Guillain-Barré syndrome. CNS Neurol. Disord. Drug Targets 5:391400.
48. Konkel, M. E.,, J. E. Christensen,, A. M. Keech,, M. R. Monteville,, J. D. Klena, and, S. G. Garvis. 2005. Identification of a fibronectin-binding domain within the Campylobacter jejuni CadF protein. Mol. Microbiol. 57:10221035.
49. Krinos, C. M.,, M. J. Coyne,, K. G. Weinacht,, A. O. Tzianabos,, D. L. Kasper, and, L. E. Comstock. 2001. Extensive surface diversity of a commensal microorganism by multiple DNA inversions. Nature 414:555558.
50. Kuroki, S.,, T. Saida,, M. Nukina,, T. Haruta,, M. Yoshioka,, Y. Kobayashi, and, H. Nakanishi. 1993. Campylobacter jejuni strains from patients with Guillain-Barré syndrome belong mostly to Penner serogroup 19 and contain β-N-acetylglucosamine residues. Ann. Neurol. 33:243247.
51. Kuusela, P.,, A. P. Moran,, T. Vartio, and, T. U. Kosunen. 1989. Interaction of Campylobacter jejuni with extracellular matrix components. Biochim. Biophys. Acta 993:297300.
52. Lastovica, A. J.,, E. A. Goddard, and, A. C. Argent. 1997. GuillainBarré syndrome in South Africa associated with Campylobacter jejuni O:41 strains. J. Infect. Dis. 176(Suppl 2):S139S143.
53. Lee, C. J.,, L. H. Lee,, C. S. Lu, and, A. Wu. 2001. Bacterial polysaccharides as vaccines—immunity and chemical characterization. Adv. Exp. Med. Biol. 491:453471.
54. Lee, L. H.,, E. Burg,, S. Baqar,, A. L. Bourgeois,, D. H. Burr,, C. P. Ewing,, T. J. Trust, and, P. Guerry. 1999. Evaluation of a truncated recombinant flagellin subunit vaccine against Campylobacter jejuni. Infect. Immun. 67:57995805.
55. Magee, A. D., and, J. Yother. 2001. Requirement for capsule in colonization by Streptococcus pneumoniae. Infect. Immun. 69:37553761.
56. Mandatori, R., and, J. L. Penner. 1989. Structural and antigenic properties of Campylobacter coli lipopolysaccharides. Infect. Immun. 57:35063511.
57. McKay, D.,, J. Fletcher,, P. Cooper, and, F. M. Thomson-Carter. 2001. Comparison of two methods for serotyping Campylobacter spp. J. Clin. Microbiol. 39:19171921.
58. McLennan, M.,, D. Ringoir,, H. Jarrell,, C. Szymanski, and, E. Gaynor. 2005. Characterization of a Campylobacter jejuni surface moiety that cross-reacts with calcofluor white: implications for surface carbohydrates, stress response, and pathogenesis, p. 87. In V. Korolik,, A. Lee,, H. Mitchell,, G. Mendz,, B. Fry, and, P. Coloe (ed.), Abstracts of the 13th International Workshop on Campylobacter, Helicobacter and Related Organisms. Griffith University Publications, Gold Coast, Australia.
59. McNally, D. J.,, H. C. Jarrell,, N. H. Khieu,, J. Li,, E. Vinogradov,, D. M. Whitfield,, C. M. Szymanski, and, J. R. Brisson. 2006. The HS:19 serostrain of Campylobacter jejuni has a hyaluronic acid-type capsular polysaccharide with a nonstoichiometric sorbose branch and O-methyl phosphoramidate group. FEBS J. 273:39753989.
60. McNally, D. J.,, H. C. Jarrell,, J. Li,, N. H. Khieu,, E. Vinogradov,, C. M. Szymanski, and, J. R. Brisson. 2005. The HS:1 serostrain of Campylobacter jejuni has a complex teichoic acid-like capsular polysaccharide with nonstoichiometric fructofuranose branches and O-methyl phosphoramidate groups. FEBS J. 272:44074422.
61. McNally, D. J.,, M. P. Lamoureux,, A. V. Karlyshev,, L. M. Fiori,, J. Li,, G. Thacker,, R. A. Coleman,, N. H. Khieu,, B. W. Wren,, J. R. Brisson,, H. C. Jarrell, and, C. M. Szymanski. 2007. Commonality and biosynthesis of the O-methyl phosphoramidate capsule modification in Campylobacter jejuni. J. Biol. Chem. 282:2856628576.
62. McSweegan, E., and, R. I. Walker. 1986. Identification and characterization of two Campylobacter jejuni adhesins for cellular and mucous substrates. Infect. Immun. 53:141148.
63. Michael, F. S.,, C. M. Szymanski,, J. Li,, K. H. Chan,, N. H. Khieu,, S. Larocque,, W. W. Wakarchuk,, J. R. Brisson, and, M. A. Monteiro. 2002. The structures of the lipooligosaccharide and capsule polysaccharide of Campylobacter jejuni genome sequenced strain NCTC 11168. Eur. J. Biochem. 269:51195136.
64. Mills, S. D.,, W. C. Bradbury, and, J. L. Penner. 1985. Basis for serological heterogeneity of thermostable antigens of Campylobacter jejuni. Infect. Immun. 50:284291.
65. Mills, S. D.,, B. Kuzniar,, B. Shames,, L. A. Kurjanczyk, and, J. L. Penner. 1992. Variation of the O antigen of Campylobacter jejuni in vivo. J. Med. Microbiol. 36:215219.
66. Moller, H. J.,, D. Heinegard, and, J. H. Poulsen. 1993. Combined Alcian blue and silver staining of subnanogram quantities of proteoglycans and glycosaminoglycans in sodium dodecyl sulfatepolyacrylamide gels. Anal. Biochem. 209:169175.
67. Moran, A. P. 1995a. Structure-bioactivity relationships of bacterial endotoxins. J. Toxicol. Toxin Rev. 14:4783.
68. Moran, A. P. 1995b. Biological and serological characterization of Campylobacter jejuni lipopolysaccharides with deviating core and lipid A structures. FEMS Immunol. Med. Microbiol. 11:121130.
69. Moran, A. P. 2007. Vaccinating humans against Campylobacter, p. 75. In Abstracts of the International Association for Food Protection 94th Annual Meeting (Orlando, Florida). International Association for Food Protection, Des Moines, IA.
70. Moran, A. P., and, H. Annuk. 2003. Recent advances in understanding biofilms of mucosae. Rev. Environ. Sci. Biotechnol. 2:121140.
71. Moran, A. P.,, H. Annuk, and, M. M. Prendergast. 2005. Antibodies induced by ganglioside-mimicking Campylobacter jejuni lipooligosaccharides recognise epitopes at the nodes of Ranvier. J. Neuroimmunol. 165:179185.
72. Moran, A. P.,, T. U. Kosunen, and, M. M. Prendergast. 2001. Biochemical and serological analysis of the heat-stable antigens involved in serotyping Campylobacter jejuni/coli. Int. J. Med. Microbiol. 291(Suppl. 31):72.
73. Moran, A. P., and, Å. Ljungh. 2003. Physico-chemical properties of extracellular polymeric substances, p. 91112. In P. Lens,, A. P. Moran,, T. O’Mahony,, P. Stoodley, and, V. O’Flaherty (ed.), Biofilms in Medicine, Industry and Environmental Biotechnology: Characteristics, Analysis and Control. IWA Publishing, London, United Kingdom.
74. Moran, A. P., and, J. L. Penner. 1999. Serotyping of Campylobacter jejuni based on heat-stable antigens: relevance, molecular basis and implications in pathogenesis. J. Appl. Microbiol. 86:361377.
75. Moran, A. P.,, J. L. Penner, and, G. O. Aspinall. 2000. Campylobacter lipopolysaccharides, p. 241257. In I. Nachamkin and, M. J. Blaser (ed.), Campylobacter, 2nd ed. ASM Press, Washington, DC.
76. Moran, A. P., and, M. M. Prendergast. 2001. Molecular mimicry in Campylobacter jejuni and Helicobacter pylori lipopolysaccharides: contribution of gastrointestinal infections to autoimmunity. J. Autoimmun. 16:241256.
77. Moran, A. P.,, U. Zähringer,, U. Seydel,, D. Ssholz,, P. Stütz, and, E. T. Rietschel. 1991. Structural analysis of the lipid A component of Campylobacter jejuni CCUG 10936 (serotype O:2) lipopolysaccharide. Description of a lipid A containing a hybrid backbone of 2-amino-2-deoxy-d-glucose and 2,3-diamino-2,3-dideoxy-d-glucose. Eur. J. Biochem. 198:459469.
78. Muldoon, J.,, A. S. Shashkov,, A. P. Moran,, J. A. Ferris,, S. N. Senchenkova, and, A. V. Savage. 2002. Structures of two polysaccharides of Campylobacter jejuni 81116. Carbohydr. Res. 337:22232229.
79. Nachamkin, I. 2002. Chronic effects of Campylobacter infection. Microbes Infect. 4:399403.
80. Nachamkin, I.,, J. Engberg,, M. Gutacker,, R. J. Meinersman,, C. Y. Li,, P. Arzate,, E. Teeple,, V. Fussing,, T. W. Ho,, A. K. Asbury,, J. W. Griffin,, G. M. McKhann, and, J. C. Piffaretti. 2001. Molecular population genetic analysis of Campylobacter jejuni HS: 19 associated with Guillain-Barré syndrome and gastroenteritis. J. Infect. Dis. 184:221226.
81. Nelson, A. L.,, A. M. Roche,, J. M. Gould,, K. Chim,, A. J. Ratner, and, J. N. Weiser. 2007. Capsule enhances pneumococcal colonization by limiting mucus-mediated clearance. Infect. Immun. 75:8390.
82. Nielsen, E. M.,, J. Engberg, and, V. Fussing. 2001. Genotypic and serotypic stability of Campylobacter jejuni strains during in vitro and in vivo passage. Int. J. Med. Microbiol. 291:379385.
83. Oldfield, N. J.,, A. P. Moran,, L. A. Millar,, M. M. Prendergast, and, J. M. Ketley. 2002. Characterization of the Campylobacter jejuni heptosyltransferase II gene, waaF, provides genetic evidence that extracellular polysaccharide is lipid A core independent. J. Bacteriol. 184:21002107.
84. Otto, M. 2006. Bacterial evasion of antimicrobial peptides by biofilm formation. Curr. Top. Microbiol. Immunol. 306:251258.
85. Papp-Szabo, E.,, M. I. Kanipes,, P. Guerry, and, M. A. Monteiro. 2005. Cell-surface α-glucan in Campylobacter jejuni 81-176. Carbohydr. Res. 340:22182221.
86. Parker, C. T.,, B. Quinones,, W. G. Miller,, S. T. Horn, and, R. E. Mandrell. 2006. Comparative genomic analysis of Campylobacter jejuni strains reveals diversity due to genomic elements similar to those present in C. jejuni strain RM1221. J. Clin. Microbiol. 44:41254135.
87. Parkhill, J.,, B. W. Wren,, K. Mungall,, J. M. Ketley,, C. Churcher,, D. Basham,, T. Chillingworth,, R. M. Davies,, T. Feltwell,, S. Holroyd,, K. Jagels,, A. V. Karlyshev,, S. Moule,, M. J. Pallen,, C. W. Penn,, M. A. Quail,, M. A. Rajandream,, K. M. Rutherford,, A. H. van Vliet,, S. Whitehead, and, B. G. Barrell. 2000. The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences. Nature 403:665668.
88. Pei, Z.,, C. Burucoa,, B. Grignon,, S. Baqar,, X. Z. Hunag,, D. J. Kopecko,, A. L. Bourgeois,, J. L. Fauchere, and, M. J. Blaser. 1998. Mutation in the peb1A locus of Campylobacter jejuni reduces interactions with epithelial cells and intestinal colonization of mice. Infect. Immun. 66:938943.
89. Penner, J. L., and, G. O. Aspinall. 1997. Diversity of lipopolysaccharide structures in Campylobacter jejuni. J. Infect. Dis. 176(Suppl. 2):S135S138.
90. Penner, J. L., and, J. N. Hennessy. 1980. Passive hemagglutination technique for serotyping Campylobacter fetus subsp. jejuni on the basis of soluble heat-stable antigens. J. Clin. Microbiol. 12:732737.
91. Poly, F.,, T. Read,, D. R. Tribble,, S. Baqar,, M. Lorenzo, and, P. Guerry. 2007. Genome sequence of a clinical isolate of Campylobacter jejuni from Thailand. Infect. Immun. 75:34253433.
92. Prendergast, M. M., and, A. P. Moran. 2000. Lipopolysaccharides in the development of the Guillain-Barré syndrome and Miller Fisher syndome forms of acute inflammatory peripheral neuropathies. J. Endotoxin Res. 6:341359.
93. Prendergast, M. M.,, D. R. Tribble,, S. Baqar,, D. S. Scott,, J. A. Ferris,, R. I. Walker, and, A. P. Moran. 2004. In vivo phase variation and serological response to lipooligosaccharide of Campylobacter jejuni in experimental human infection. Infect. Immun. 72:916922.
94. Preston, M. A., and, J. L. Penner. 1987. Structural and tigneic properties of lipopolysaccharides from serotype reference strains of Campylobacter jejuni. Infect. Immun. 55:18061812.
95. Prokhorova, T. A.,, P. N. Nielsen,, J. Petersen,, T. Kofoed,, J. S. Crawford,, C. Morsczeck,, A. Boysen, and, P. Schrotz-King. 2006. Novel surface polypeptides of Campylobacter jejuni as traveller’s diarrhoea vaccine candidates discovered by proteomics. Vaccine 24:64466455.
96. Rahman, M. M.,, J. Guard-Petter,, K. Asokan,, C. Hughes, and, R. W. Carlson. 1999. The structure of the colony migration factor from pathogenic Proteus mirabilis. A capsular polysaccharide that facilitates swarming. J. Biol. Chem. 274:2299322998.
97. Reeser, R. J.,, R. T. Medler,, S. J. Billington,, B. H. Jost, and, L. A. Joens. 2007. Characterization of Campylobacter jejuni biofilms under defined growth conditions. Appl. Environ. Microbiol. 73:19081913.
98. Richardson, P. T., and, S. F. Park. 1997. Integration of heterologous plasmid DNA into multiple sites on the genome of Campylobacter coli following natural transformation. J. Bacteriol. 179:18091812.
99. bRoberts, I. S. 1996. The biochemistry and genetics of capsular polysaccharide production in bacteria. Annu. Rev. Microbiol. 50:285315.
100. Rowe, S.,, N. Hodson,, G. Griffiths, and, I. S. Roberts. 2000. Regulation of the Escherichia coli K5 capsule gene cluster: evidence for the roles of H-NS, BipA, and integration host factor in regulation of group 2 capsule gene clusters in pathogenic E. coli. J. Bacteriol. 182:27412745.
101. Schmidt, M. A., and, K. Jann. 1982. Phosholipid substitution of capsular (K) polysaccharide antigens from Escherichia coli causing extra-intestinal infections. FEMS Microbiol. Lett. 14:6974.
102. Schrotz-King, P.,, T. A. Prokhorova,, P. N. Nielsen,, J. S. Crawford, and, C. Morsczeck. 2007. Campylobacter jejuni proteomics for new travellers’ diarrhoea vaccines. Travel. Med. Infect. Dis. 5:106109.
103. Scott, D. A. 1997. Vaccines against Campylobacter jejuni. J. Infect. Dis. 176(Suppl. 2):S183S188.
104. Shea, S. M. 1971. Lanthanum staining of the surface coat of cells. Its enhancement by the use of fixatives containing Alcian blue or cetylpyridinium chloride. J. Cell Biol. 51:611620.
105. Sizemore, D. R.,, B. Warner,, J. Lawrence,, A. Jones, and, K. P. Killeen. 2006. Live, attenuated Salmonella typhimurium vectoring Campylobacter antigens. Vaccine 24:37933803.
106. Snelling, W. J.,, M. Matsuda,, J. E. Moore, and, J. S. Dooley. 2005a. Campylobacter jejuni. Lett. Appl. Microbiol. 41:297302.
107. Snelling, W. J.,, J. P. McKenna,, D. M. Lecky, and, J. S. Dooley. 2005b. Survival of Campylobacter jejuni in waterborne protozoa. Appl. Environ. Microbiol. 71:55605571.
108. Spinosa, M. R.,, C. Progida,, A. Tala,, L. Cogli,, P. Alifano, and, C. Bucci. 2007. Neisseria meningitidis capsule is important for intracellular survival in human cells. Infect. Immun. 75:35943603.
109. Stintzi, A. 2003. Gene expression profile of Campylobacter jejuni in response to growth temperature variation. J. Bacteriol. 185:20092016.
110. Stintzi, A.,, D. Marlow,, K. Palyada,, H. Naikare,, R. Panciera,, L. Whitworth, and, C. Clarke. 2005. Use of genome-wide expression profiling and mutagenesis to study the intestinal lifestyle of Campylobacter jejuni. Infect. Immun. 73:17971810.
111. Stintzi, A., and, L. Whitworth. 2003. Investigation of the Campylobacter jejuni cold-shock response by global transcript profiling. Genome Lett. 2:1827.
112. Su, J.,, J. Yang,, D. Zhao,, T. H. Kawula,, J. A. Banas, and, J. R. Zhang. 2007. Genome-wide identification of Francisella tularensis virulence determinants. Infect. Immun. 75:30893101.
113. Sussman, M. 1997. Capsules of Esherichia coli, p. 114143. In M. Sussman (ed.), Esherichia coli: Mechanisms of Virulence. Cambridge University Press, Cambridge, United Kingdom.
114. Szymanski, C. M.,, F. St Michael,, H. C. Jarrell,, J. Li,, M. Gilbert,, S. Larocque,, E. Vinogradov, and, J. R. Brisson. 2003. Detection of conserved N-linked glycans and phase variable lipo-oligosaccharides and capsules from Campylobacter cells by mass spectrometry and high resolution magic angle spinning NMR spectroscopy. J. Biol. Chem. 278:2450924520.
115. Taylor, C. M, and, I. S. Roberts. 2005. Capsular polysaccharides and their role in virulence. Contrib. Microbiol. 12:5566.
116. Trachoo, N.,, J. F. Frank, and, N. J. Stern. 2002. Survival of Campylobacter jejuni in biofilms isolated from chicken houses. J. Food Prot. 65:11101116.
117. Tzianabos, A. O., and, D. L. Kasper. 2002. Role of T cells in abscess formation. Curr. Opin. Microbiol. 5:9296.
118. Walker, R. I. 2005. Considerations for development of whole cell bacterial vaccines to prevent diarrheal diseases in children in developing countries. Vaccine 16:33693385.
119. Wassenaar, T. M.,, B. N. Fry,, A. J. Lastovica,, J. A. Wagenaar,, P. J. Coloe, and, B. Duim. 2000. Genetic characterization of Campylobacter jejuni O:41 isolates in relation with Guillain-Barré syndrome. J. Clin. Microbiol. 38:874876.
120. Whitfield, C. 2006. Biosynthesis and assembly of capsular polysaccharides in Escherichia coli. Annu. Rev. Biochem. 75:3968.
121. Whitfield, C., and, I. S. Roberts. 1999. Structure, assembly and regulation of expression of capsules in Escherichia coli. Mol. Microbiol. 31:13071319.
122. Woodall, C. A.,, M. A. Jones,, P. A. Barrow,, J. Hinds,, G. L. Mars-den,, D. J. Kelly,, N. Dorrell,, B. W. Wren, and, D. J. Maskell. 2005. Campylobacter jejuni gene expression in the chick cecum: evidence for adaptation to a low-oxygen environment. Infect. Immun. 73:52785285.
123. Wyszynska, A.,, A. Raczko,, M. Lis, and, E. K. Jagusztyn-Krynicka. 2004. Oral immunization of chickens with avirulent Salmonella vaccine strain carrying C. jejuni 72Dz/92 cjaA gene elicits specific humoral immune response associated with protection against challenge with wild-type Campylobacter. Vaccine 22:13791389.
124. Yi, K.,, A. W. Rasmussen,, S. K. Gudlavalleti,, D. S. Stephens, and, I. Stojiljkovic. 2004. Biofilm formation by Neisseria meningitidis. Infect. Immun. 72:61326138.
125. Yun, M.,, S. Yoon,, Y. Shin,, K. H. Chun, and, J. E. Nam Shin. 2004. Synthesis of 2′-azidoethyl trisaccharide, α-d-Gal-(1→2)-6-deoxy-α-d-altro-Hep p-(1→3)-β-d-GlcNAc, an O-antigenic repeating unit of C. jejuni O:23 and O:36. Arch. Pharm. Res. 27:143150.
126. Ziprin, R. L.,, M. E. Hume,, C. R. Young, and, R. B. Harvey. 2002. Inoculation of chicks with viable non-colonizing strains of Campylobacter jejuni: evaluation of protection against a colonizing strain. Curr. Microbiol. 44:221223.

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