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Chapter 31 : Natural Competence and Transformation in

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Abstract:

Over 40 naturally competent bacterial species are known, and that number continues to grow. Almost all competent bacteria studied to date use components of the type II secretion/type IV pilus biogenesis family of proteins for transformation, with slight differences between the transformation machinery in each system. The two known exceptions are , which requires components of a type IV secretion/conjugation system for transformation, and , in which some strains use both type II-like and type IV-like secretion systems. Three different mechanisms may contribute to generating genetic diversity. The first is local sequence change such as nucleotide substitutions or small insertions or deletions of one or a few nucleotides. Another mechanism is DNA rearrangement, where related sequences in the genome undergo recombination to create novel fusion genes, and duplicate or delete DNA segments. Finally, diversity can be generated by horizontal acquisition of DNA through mechanisms such as natural transformation, conjugation, and transduction. Given the natural competence of most species, antibiotic resistance is probably spread in part through transformation. Several species of are naturally competent for transformation, including and . The candidate gene approach identified recombinase RecA as having a function in transformation. recA mutants were unable to be transformed to streptomycin resistance in three strains tested, 81-176, VC83, and 81-116. Modification of proteins by addition of an -linked glycan is due to the activity of a number of genes found in the locus.

Citation: Wiesner R, Dirita V. 2008. Natural Competence and Transformation in , p 559-570. In Nachamkin I, Szymanski C, Blaser M (ed), , Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555815554.ch31
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Figure 1.

Conserved locus encoding type II secretion system genes from several strains of . See text for details.

Citation: Wiesner R, Dirita V. 2008. Natural Competence and Transformation in , p 559-570. In Nachamkin I, Szymanski C, Blaser M (ed), , Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555815554.ch31
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Tables

Generic image for table
Table 1.

Conserved proteins associated with transformation in naturally competent bacteria

Citation: Wiesner R, Dirita V. 2008. Natural Competence and Transformation in , p 559-570. In Nachamkin I, Szymanski C, Blaser M (ed), , Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555815554.ch31

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