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Chapter 100 : Isolation of and Amoebae from Water Samples

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Isolation of and Amoebae from Water Samples, Page 1 of 2

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Abstract:

Many studies describe ability to survive and to multiply in about 30 species of amoebae, especially , , and , and in two species of ciliates. This chapter describes isolation of from hospital water supplies and from air conditioning systems during periodic controls sometimes performed after water disinfection. The culture of amoebae was also performed, and the interactions with legionellae were studied. was isolated from 251 of 471 (53.3%) hospital water samples (239 hot and 12 cold), corresponding to 11 of 12 (91.7%) hospitals. Amoebae alone were isolated from 27 of 471 (5.7%) samples, while alone were isolated from 196 of 471 (41.6%) samples. The distribution of and amoebae in all water samples in relation to temperature have been discussed in the chapter. The results of this study confirmed those observed by other authors reporting similar data for isolation (55%). In conclusion, the isolation rate was higher than that of amoebae from hospital water supplies, but amoebae were found in higher percents from air conditioning systems (64%, the despite limited number of samples examined) where was absent.

Citation: Franzin L, Cabodi D, Bonfrate N. 2006. Isolation of and Amoebae from Water Samples, p 423-426. In Cianciotto N, Kwaik Y, Edelstein P, Fields B, Geary D, Harrison T, Joseph C, Ratcliff R, Stout J, Swanson M (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555815660.ch100

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Figures

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FIGURE 1

Distribution of and amoebae in water samples.

Citation: Franzin L, Cabodi D, Bonfrate N. 2006. Isolation of and Amoebae from Water Samples, p 423-426. In Cianciotto N, Kwaik Y, Edelstein P, Fields B, Geary D, Harrison T, Joseph C, Ratcliff R, Stout J, Swanson M (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555815660.ch100
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References

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1. Barbaree, J. M.,, B. S. Fields,, J. C. Feeley,, G. W. Gorman, and, W. T. Martin. 1986. Isolation of protozoa from water associated with a le-gionellosis outbreak and demonstration of intra-cellular multiplication of Legionella pneumophila. Appl. Environ. Microbiol. 51:422424.
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3. Fields, B. S. 1993. Legionella and protozoa: interaction of a pathogen and its natural host, p. 129136. In J. M. Barbaree,, R. F. Breiman, and, A. P. Du-four (ed.). Legionella: Current Status and Emerging Perspectives. ASM Press, Washington, D.C.
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5. Greub, G., and, D. Raoult. 2004. Microorganisms resistant to free–living amoebae. Clin. Microbiol. Rev. 17:413433.
6. Harb, O. S., and, Y. A. Kwaik. 2000. Interaction of Legionella pneumophila with protozoa provides lessons. ASM News 66:609616.
7. Patterson, W. J.,, J. Hay,, D. V. Seal and, J. C. McLuckie. 1997. Colonization of transplant unit water supplies with Legionella and protozoa: precautions required to reduce the risk of legionel-losis. J. Hosp. Infect. 37:717.
8. Rohr, U.,, S. Weber,, R. Michel,, F. Selenka, and, M. Wilhelm. 1998. Comparison of free-living amoebae in hot water systems of hospitals with isolates from moist sanitary areas by identifying genera and determining temperature tolerance. Appl. Environ. Microbiol. 64:18221824.
9. Rowbotham, T. J. 1980. Preliminary report on the pathogenicity of Legionella pneumophila for freshwater and soil amoebae. J. Clin. Pathol. 33: 11791183.
10. Visvesvara, G. S. 1999. Pathogenic and opportunist free-living amebae, p. 13831390. In P. R. Murray,, E. Y. Baron,, M. A. Pfaller,, F. C. Tenover, and, R. H. Yolken (ed.). Manual of Clinical Microbiology, 7th ed. ASM Press, Washington, D.C.

Tables

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TABLE 1

Simultaneous occurrence of and amoebae on hospital water samples

Citation: Franzin L, Cabodi D, Bonfrate N. 2006. Isolation of and Amoebae from Water Samples, p 423-426. In Cianciotto N, Kwaik Y, Edelstein P, Fields B, Geary D, Harrison T, Joseph C, Ratcliff R, Stout J, Swanson M (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555815660.ch100

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