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Chapter 107 : Evaluation of the Dynal Biotech Immunomagnetic Separation Method versus Conventional Culture for the Isolation of Serogroup 1 from Water Samples

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Evaluation of the Dynal Biotech Immunomagnetic Separation Method versus Conventional Culture for the Isolation of Serogroup 1 from Water Samples, Page 1 of 2

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Abstract:

Environmental monitoring of water systems for is performed for routine microbiological surveillance and outbreak investigations. The recovery of from water samples using conventional culture on selective media can be improved by sample concentration and/or sample decontamination techniques, but there is a reduction in recovery associated with these processes. A new immunoseparation method has been developed to improve the recovery of by concentrating and selectively separating spp. directly from water samples using immunomagnetic separation (IMS). Immunomagnetic separation has been used successfully for detection of other pathogens including spp. The authors conducted the study in two phases and evaluated the IMS process alone and in combination with concentration and/or acid pretreatment in detecting spp. and serogroup 1 (sg 1). The IMS procedure was performed using the BeadRetriever apparatus (Dynal Biotech Ltd., Preston, United Kingdom) for automated IMS using Dynabeads anti-Legionella (Dynal Biotech Ltd.). With the 11 known potable water samples, there was concordance (100% sensitivity) of culture results between the two methods, regardless of concentration or pretreatment of samples. The unconcentrated, untreated samples showed the greatest agreement in CFU/ml. Compared to conventional culture for sg 1 only, the IMS method demonstrated a specificity of 98.5% (65 were negative and 2 were false-positive).

Citation: M. Mietzner S, E. Stout J, L. Shannon J, L. Yu V, R. Wareing D. 2006. Evaluation of the Dynal Biotech Immunomagnetic Separation Method versus Conventional Culture for the Isolation of Serogroup 1 from Water Samples, p 449-452. In Cianciotto N, Kwaik Y, Edelstein P, Fields B, Geary D, Harrison T, Joseph C, Ratcliff R, Stout J, Swanson M (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555815660.ch107

Key Concept Ranking

Legionella pneumophila
0.76339406
Polymyxin B
0.525
0.76339406
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References

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1. Allegheny County Health Department. 1997. Approaches to Prevention and Control of Legionella Infection in Allegheny County Health Care Facilities, 2nd ed., p. 115. Allegheny County Health Department, Pittsburgh, Pa. (http://www.legionella.org.)
2. Best, M.,, V. L. Yu,, J. Stout,, A. Goetz,, R. R. Muder, and, F. Taylor. 1983. Legionellaceae in the hospital water supply: epidemiological link with disease and evaluation of a method of control of nosocomial Legionnaires’ disease and Pittsburgh pneumonia. Lancet 2: 307-310.
3. Duncanson, P.,, D. R. A. Wareing, and, O. Jones. 2003. Application of an automated im-munomagnetic separation-enzyme immunoassay for the detection of Salmonella spp. during an outbreak associated with a retail premises. Lett. Appl. Microbiol. 37:15.
4. Kool, J. L.,, D. Bergmire-Sweat,, J. C. Butler,, E. W. Brown,, D. J. Peabody,, D. S. Massi,, J. C. Carpenter,, J. M. Pruckler,, R. F. Benson, and, B. S. Fields. 1999. Hospital characteristics associated with colonization of water systems by Legionella and risk of nosocomial Legionnaire’s disease: a cohort study of 15 hospitals. Infect. Control Hosp. Epidemiol. 20:798805.
5. State of Maryland Department of Health and Mental Hygiene. 2000. Report of the Maryland Scientific Working Group to Study Legionella in Water Systems in Healthcare Institutions.
6. Ta, A. C.,, J. E. Stout,, V. L. Yu, and, M. M. Wa-gener. 1995. Comparison of culture methods for monitoring Legionella species in hospital potable water systems and recommendations for standardization of such methods. J. Clin. Microbiol. 33:21182123.

Tables

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TABLE 1

Results from the phase I study. The IMS method recovered serogroup 1 from potable water samples at concentrations similar to conventional culture

Citation: M. Mietzner S, E. Stout J, L. Shannon J, L. Yu V, R. Wareing D. 2006. Evaluation of the Dynal Biotech Immunomagnetic Separation Method versus Conventional Culture for the Isolation of Serogroup 1 from Water Samples, p 449-452. In Cianciotto N, Kwaik Y, Edelstein P, Fields B, Geary D, Harrison T, Joseph C, Ratcliff R, Stout J, Swanson M (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555815660.ch107
Generic image for table
TABLE 2

Results from phase II prospective study of potable water samples

Citation: M. Mietzner S, E. Stout J, L. Shannon J, L. Yu V, R. Wareing D. 2006. Evaluation of the Dynal Biotech Immunomagnetic Separation Method versus Conventional Culture for the Isolation of Serogroup 1 from Water Samples, p 449-452. In Cianciotto N, Kwaik Y, Edelstein P, Fields B, Geary D, Harrison T, Joseph C, Ratcliff R, Stout J, Swanson M (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555815660.ch107

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