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Chapter 110 : Field Evaluation of the Binax Equate Test Kit for Enumeration of Serogroup 1 in Cooling Water Samples

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Field Evaluation of the Binax Equate Test Kit for Enumeration of Serogroup 1 in Cooling Water Samples, Page 1 of 2

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Abstract:

This chapter investigates on the efficacy of detection of serogroup 1 (sg 1) in cooling waters using the Binax Equate water test. The test kit offers a rapid detection method (45 min) for sg 1 in environmental water samples. A high proportion of Binax positive results were also between 1.0 and 1.9 OD. species were cultured from 22% (n = 31) of all cooling water samples. Presumptive spp. were isolated from 42.9% of all cooling water samples. Two samples grew to confluence, indicating very high concentrations of spp. in those samples. Presumptive was isolated from 21.9% of all cooling water samples. The post-treatment results for the heat-treated sample showed that the average absorbance decreased following the treatment. A large number of false-positive Binax results were recorded whether or not spp. were cultured from the sample. Alternatively, the test also recognized heat-killed cells and false-positive results may have been due to the presence of dead in the cooling systems. The results suggest that this system lacks the required specificity for routine use in cooling towers or other water systems. It seems likely that the test method would produce false-positive results in systems where disinfection was applied. False-positive results from cooling tower samples in this study may have been due to the presence of killed sg 1. The results point to the necessity of indication of viability in future environmental tests for .

Citation: Benovic A, Bentham R. 2006. Field Evaluation of the Binax Equate Test Kit for Enumeration of Serogroup 1 in Cooling Water Samples, p 460-462. In Cianciotto N, Kwaik Y, Edelstein P, Fields B, Geary D, Harrison T, Joseph C, Ratcliff R, Stout J, Swanson M (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555815660.ch110

Key Concept Ranking

Legionella pneumophila
0.7613637
Pseudomonas fluorescens
0.6590909
0.7613637
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Figures

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FIGURE 1

Distribution of the Binax absorbance readings of the cooling tower water samples.

Citation: Benovic A, Bentham R. 2006. Field Evaluation of the Binax Equate Test Kit for Enumeration of Serogroup 1 in Cooling Water Samples, p 460-462. In Cianciotto N, Kwaik Y, Edelstein P, Fields B, Geary D, Harrison T, Joseph C, Ratcliff R, Stout J, Swanson M (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555815660.ch110
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Image of FIGURE 2
FIGURE 2

The detection of killed sg 1. The sg 1 load of each treatment prior to undergoing treatment was: Heat treatment 10 dilution = 7.6 × 10 CFU; Heat treatment 10 dilution = 3.85 × 10 CFU; Heat treatment + sonication 10 dilution = 7.65 × 10 CFU.

Citation: Benovic A, Bentham R. 2006. Field Evaluation of the Binax Equate Test Kit for Enumeration of Serogroup 1 in Cooling Water Samples, p 460-462. In Cianciotto N, Kwaik Y, Edelstein P, Fields B, Geary D, Harrison T, Joseph C, Ratcliff R, Stout J, Swanson M (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555815660.ch110
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References

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1. Standards Australia. AS/NZS 3896:1998:Wa-ters: Examination for Legionellae Including Legionella Pneumophila. Standards Australia, Sydney Australia.

Tables

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TABLE 1

Summary of microbiological analyses and positive cooling water samples and systems

Citation: Benovic A, Bentham R. 2006. Field Evaluation of the Binax Equate Test Kit for Enumeration of Serogroup 1 in Cooling Water Samples, p 460-462. In Cianciotto N, Kwaik Y, Edelstein P, Fields B, Geary D, Harrison T, Joseph C, Ratcliff R, Stout J, Swanson M (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555815660.ch110

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