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Chapter 111 : Method Development for Detection in Metalworking Fluids

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Method Development for Detection in Metalworking Fluids, Page 1 of 2

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Abstract:

Metalworking fluids (MWFs) are highly susceptible to microbiological contamination from the environment due to high water content, available nutrients, and optimum growth temperature for most environmental microorganisms. Outbreaks of Pontiac fever and Legionnaires' disease were associated with MWFs at two unrelated automotive facilities. and serogroup 1 (sg 1) were identified as the probable causative organisms. The objective of this chapter was to develop a selective and sensitive culture method for detection of in MWFs in order to prevent and control possible occupational health-related problems. Recovery efficiency was evaluated by spiking MWF field samples, highly contaminated (10 to 10/ml) with nonlegionella organisms with high levels (10 to 10/ml) of sg 1 and . Several sample treatment conditions to reduce the interference of the high background population, including acidification, heat treatment, and use of antibiotics in the recovery medium, were evaluated.

Citation: Rossmoore K, Rossmoore L, Cuthbert C. 2006. Method Development for Detection in Metalworking Fluids, p 463-464. In Cianciotto N, Kwaik Y, Edelstein P, Fields B, Geary D, Harrison T, Joseph C, Ratcliff R, Stout J, Swanson M (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555815660.ch111

Key Concept Ranking

Legionella feeleii
0.95
Legionella pneumophila
0.95
0.95
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References

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1. Bopp, C. A.,, J. W. Summer,, G. K. Morris, and, J. G. Wells. 1981. Isolation of Legionella spp. from environmental water samples by low-pH treatment and use of a selective medium. J. Clin. Microbiol. 13:174719.
2. Edelstein, P. H.,, J. B. Snitzer, and, J. A. Bridge. 1982. Enhancement of recovery of Legionella pneumophila from contaminated respiratory tract specimens by heat. J. Clin. Microbiol. 16:10611065.
3. Herwaldt, L. A.,, G. W. Gorman,, T. McGrath,, S. Toma,, B. Brake,, A. W. Hightower,, J. Jones,, A. L. Reingold,, P. A. Boxer,, P. W. Tang,, C. W. Moss,, H. Wilkinson,, D. J. Brenner,, A. G. Steigerwalt, and, C. V. Broome. 1984. A new Legionella species, Legionella feeleii species nova, causes Pontiac fever in an automobile plant. Ann. Intern. Med. 100:333338.
4. Passman, F. J., and, H. W. Rossmoore. 2002. Reassessing the health risks associated with employee exposure to metalworking fluid microbes. Lubrication Eng. 58:3038.
5. Rossmoore, H. W.,, L. A. and,, C. E. Young. 1987. Microbial ecology of an automotive engine plant. Int. Biodeterioration Biodegradation 1:255268.

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