1887

Chapter 13 : Specific Detection of in Samples from Patients with Community-Acquired Pneumonia by PCR and a Colorimetric Detection System (Reverse Dot Blot)

MyBook is a cheap paperback edition of the original book and will be sold at uniform, low price.

Ebook: Choose a downloadable PDF or ePub file. Chapter is a downloadable PDF file. File must be downloaded within 48 hours of purchase

Buy this Chapter
Digital (?) $15.00

Preview this chapter:
Zoom in
Zoomout

Specific Detection of in Samples from Patients with Community-Acquired Pneumonia by PCR and a Colorimetric Detection System (Reverse Dot Blot), Page 1 of 2

| /docserver/preview/fulltext/10.1128/9781555815660/9781555813901_Chap13-1.gif /docserver/preview/fulltext/10.1128/9781555815660/9781555813901_Chap13-2.gif

Abstract:

Despite extensive efforts, the etiology of about 40 to 60% of community-acquired pneumonia remains unclear; a number of these cases are attributed to . The incidence in this group may be as high as 38%. Cultivation is still supposed to be the gold standard, but there is doubt about the sensitivity in clinical practice. Researchers established a new method for the detection of by using PCR to amplify genomic ribosomal DNA and a reverse dot blot for the detection of and non-pneumophila species. The authors used a multiplex-PCR and the coamplification of human DNA (pyruvate dehydrogenase gene) as an internal control to avoid false-positive results. To avoid false-positive results, incubation with uracil-N-glycosylase was used, and for avoidance of false-negative results the coamplification of the pyruvate dehydrogenase gene was used, creating an amplificate of 185 bp in length in each PCR. The authors used the established method to investigate clinical specimens of 93 patients taken out of a prospective randomized study about the epidemiology of community-acquired pneumonia in Berlin, Germany, from 1991 to 1992. They were able to demonstrate the ability to detect using PCR in conjunction with reverse dot blotting. This method allows the enhanced detection of non-pneumophila species in addition to established methods. Interpretation of the results for the incidence of non-pneumophila infections remains controversial because of a lack of facts about pathogenity of this group and comparable results in other studies.

Citation: Kircher J, Alexander K, Rolfs A. 2006. Specific Detection of in Samples from Patients with Community-Acquired Pneumonia by PCR and a Colorimetric Detection System (Reverse Dot Blot), p 51-52. In Cianciotto N, Kwaik Y, Edelstein P, Fields B, Geary D, Harrison T, Joseph C, Ratcliff R, Stout J, Swanson M (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555815660.ch13

Key Concept Ranking

Legionella pneumophila
1.0830573
Pyruvate Dehydrogenase
0.73130494
1.0830573
Highlighted Text: Show | Hide
Loading full text...

Full text loading...

References

/content/book/10.1128/9781555815660.ch13
1. Bartlett, J. G. 2004. Diagnostic test for etiologic agents of community-acquired pneumonia. Infect. Dis. Clin. North Am. 18:809827.
2. Marston, B. J., J. F. Plouffe, T. M. File,, Jr., B.A. Hackman,, S. J. Salstrom,, H. B. Lipman,, M. S. Kolczak, and, R. F. Breiman, andThe Community-Based Pneumonia Incidence Study Group. 1997. Incidence of community-acquired pneumonia requiring hospitalization. Results of a population-based active surveillance Study in Ohio. Arch. Intern. Med. 157:17091718.
3. Robinson P. N.,, B. Heidrich,, F. Tiecke,, F. J. Fehrenbach,, and A. Rolfs. 1996. Species-specific detection of Legionella using polymerase chain reaction and reverse dot-blotting. FEMS Microbiol. Lett. 140:111119.
4. Roig, J.,, M. Sabria,, and M. L. Pedro-Botet. 2003. Legionella spp.: community acquired and nosocomial infections. Curr. Opin. Infect. Dis. 16:145151.
5. Schaberg, T., and H. Lode. 1991. Clinical aspects and diagnosis of community-acquired pneumonia. Dtsch. Med.Wochenschr. 116:18771880. [In German.]

This is a required field
Please enter a valid email address
Please check the format of the address you have entered.
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error