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Chapter 14 : Transcription-Mediated Amplification Assay for Detection of Legionella pneumophila in Samples from Patients with Community-Acquired Pneumonia
Transcription-Mediated Amplification Assay for Detection of Legionella pneumophila in Samples from Patients with Community-Acquired Pneumonia, Page 1 of 2< Previous page Next page > /docserver/preview/fulltext/10.1128/9781555815660/9781555813901_Chap14-1.gif /docserver/preview/fulltext/10.1128/9781555815660/9781555813901_Chap14-2.gif
Diagnosis of acute Legionella pneumophila in patients with community-acquired pneumonia can be time-consuming and difficult to confirm using current diagnostic tests. A research prototype assay using Gen-Probe's proprietary transcription-mediated amplification (TMA) technology was evaluated for detection of L. pneumophila in throat swabs from patients with documented community-acquired pneumonia. The TMA L. pneumophila assay procedure includes target capture, TMA, and the hybridization protection assay. Amplified methods are generally more sensitive than other laboratory methods, particularly for fastidious organisms such as Legionella. Therefore, in addition to the initial TMA L. pneumophila assay that targets a specific region of L. pneumophila rRNA, another target was utilized in an alternate region of L. pneumophila rRNA as a confirmatory TMA-based assay.