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Chapter 17 : Serologic Study of an Outbreak of Legionnaires’ Disease: Variation of Sensitivity Associated with the Subgroup of sg1 Antigen Used and Evidence of Concurrent Reactivity to Other Atypical Pneumonia Agents

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Serologic Study of an Outbreak of Legionnaires’ Disease: Variation of Sensitivity Associated with the Subgroup of sg1 Antigen Used and Evidence of Concurrent Reactivity to Other Atypical Pneumonia Agents, Page 1 of 2

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Abstract:

An outbreak of Legionnaires' disease (LD) occurred in August 2004 in Sweden in the town of Lidköping, by Lake Vänern. Two genotypes of serogroup (sg) 1 were found by culture in patient samples, and one belonging to subgroup Benidorm (genotype A) and the other to subgroup Bellingham (genotype B). The indirect immunofluorescent antibody technique (IFAT) is still a standard method for antibody assay in patients with legionellosis, allowing for the screening of antibodies against several species and subtypes of . A serological study was therefore undertaken to assess the impact of the legionella outbreak on all pneumonia cases that occurred in the town. Patient samples were also tested for antibodies against other atypical pneumonia agents, i.e. and . Local experience in the laboratory using more than one subgroup of antigen had been shown earlier to increase sensitivity. Optimal sensitivity of immunofluorescent antibody tests in serological diagnosis of sg 1 infections requires the use of an antigen subgroup that is in agreement with the antigenic setup of the epidemic strain.

Citation: Bernander S, Claesson B, Hjelm E, Svensson N, Hjorth M. 2006. Serologic Study of an Outbreak of Legionnaires’ Disease: Variation of Sensitivity Associated with the Subgroup of sg1 Antigen Used and Evidence of Concurrent Reactivity to Other Atypical Pneumonia Agents, p 63-67. In Cianciotto N, Kwaik Y, Edelstein P, Fields B, Geary D, Harrison T, Joseph C, Ratcliff R, Stout J, Swanson M (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555815660.ch17

Key Concept Ranking

Legionella pneumophila
0.7407935
Atypical Pneumonia
0.72368413
0.7407935
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Figures

Image of FIGURE 1
FIGURE 1

Diagram showing the course of the Lidköping outbreak. Black: 32 confirmed and Presumptive cases of LD. Shaded: 106 other pneumonia cases form the same period. The axis shows number of cases, and the axis shows day of onset.

Citation: Bernander S, Claesson B, Hjelm E, Svensson N, Hjorth M. 2006. Serologic Study of an Outbreak of Legionnaires’ Disease: Variation of Sensitivity Associated with the Subgroup of sg1 Antigen Used and Evidence of Concurrent Reactivity to Other Atypical Pneumonia Agents, p 63-67. In Cianciotto N, Kwaik Y, Edelstein P, Fields B, Geary D, Harrison T, Joseph C, Ratcliff R, Stout J, Swanson M (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555815660.ch17
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References

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1. Claesson, B. E. B.,, H. Enroth,, S. Elowson, and, M. Hellgren-Leonardsson. 2004. Evaluation of diagnostic methods for Mycoplasma pneumoniae and Chlamydophila pneumoniae using serology, PCR and the BD Probe Tec ET System. ECCMID, Prague, May 2004. Clin. Microbiol. Infect. 10:67.
2. Darelid, J.,, H. Hallander,, S. Löfgren,, B.-E. Malmvall, and, A.-M. Olinder-Nielsen. 2001. Community spread of Legionella pneumophila serogroup 1 in temporal relation to a nosocomial outbreak. Scand. J. Infect. Dis. 33:194199.
3. Gaia, V.,, N. K. Fry,, B. Afshar,, P. C. Lück,, H. Meugnier,, J. Etienne,, R. Peduzzi, and, T. G. Harrison. 2005. Consensus sequence-based scheme for epidemiological typing of clinical and environmental isolates of Legionella pneumophila. J. Clin. Microbiol. 43:20472052.
4. Helbig, J. H.,, J. B. Kurtz,, M. Castellani Pastoris,, C. Pelaz, and, P. C. Lück. 1997. Antigenic polysaccharide components of Legionella pneumophila recognized by monoclonal antibodies: possibilities and limitations for division of the species into serogroups. J. Clin. Microbiol. 35:28412845.
5. Helbig, J. H.,, P. C. Lück,, Y.A. Knirel,, W. Witzleb, and, U. Zähringer. 1995. Molecular characterization of a virulence-associated epitope on the lipopolysaccharide of Legionella pneumophila serogroup 1. Epidemiol. Infect. 115:7178.
6. Kallings, I. and, K. Nordström. 1983. The pattern of immunoglobulins with special reference to IgM in Legionnaires’ disease patients during a 2 year follow-up period. Zbl. Bakt. Hyg., I.Abt. Orig. A 255:2732.
7. Lindsay, D. S. J.,, W. H. Abraham,, W. Findlay,, P. Christie,, F. Johnston, and, G. F. S. Edwards. 2004. Laboratory diagnosis of Legionnaires’ disease due to Legionella pneumophila serogroup 1: comparison of phenotypic and genotypic methods. J. Med. Microbiol. 53:183187.
8. Marshall, L. E.,, T. C. Boswell, and, G. Kudesia. 1994. False positive legionella serology in campylobacter infection: campylobacter serotypes, duration of antibody response and elimination of cross-reactions in the indirect fluorescent antibody test. Epidemiol. Infect. 112:347357.
9. Wentworth, B. B. and, H. E. Stiefel. 1982. Studies on the specificity of Legionella serology. J. Clin. Microbiol. 15:961963.
10. Wilkinson, H.W.,, A. L. Reingold,, B. J. Brake,, D. L. McGiboney,, G. W. Gorman, and, C.V. Broome. 1983. Reactivity of serum from patients with suspected legionellosis against 29 antigens of Legionellaceae and Legionella-like organisms by indirect immunofluorescense assay. J. Infect. Dis. 147:2331.
11. Zuravleff, J. J.,, V. L. Yu,, J.W. Shonnard,, B. K. Davis, and, J. D. Rihs. 1983. Diagnosis of Legionnaires’ disease: an update of laboratory methods with new emphasis on isolation by culture. JAMA 250:19811985.

Tables

Generic image for table
TABLE 1

Results of MAb subgrouping and sequence-based typing of six genes

Citation: Bernander S, Claesson B, Hjelm E, Svensson N, Hjorth M. 2006. Serologic Study of an Outbreak of Legionnaires’ Disease: Variation of Sensitivity Associated with the Subgroup of sg1 Antigen Used and Evidence of Concurrent Reactivity to Other Atypical Pneumonia Agents, p 63-67. In Cianciotto N, Kwaik Y, Edelstein P, Fields B, Geary D, Harrison T, Joseph C, Ratcliff R, Stout J, Swanson M (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555815660.ch17
Generic image for table
TABLE 2

Results of testing 12 patients serologically with IFAT using 5 subgroups of sg 1 (Bellingham, Benidorm, Knoxville, OLDA, and Philadelphia)

Citation: Bernander S, Claesson B, Hjelm E, Svensson N, Hjorth M. 2006. Serologic Study of an Outbreak of Legionnaires’ Disease: Variation of Sensitivity Associated with the Subgroup of sg1 Antigen Used and Evidence of Concurrent Reactivity to Other Atypical Pneumonia Agents, p 63-67. In Cianciotto N, Kwaik Y, Edelstein P, Fields B, Geary D, Harrison T, Joseph C, Ratcliff R, Stout J, Swanson M (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555815660.ch17

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