Full text loading...
Chapter 17 : Serologic Study of an Outbreak of Legionnaires’ Disease: Variation of Sensitivity Associated with the Subgroup of Legionella pneumophila sg1 Antigen Used and Evidence of Concurrent Reactivity to Other Atypical Pneumonia Agents
Serologic Study of an Outbreak of Legionnaires’ Disease: Variation of Sensitivity Associated with the Subgroup of Legionella pneumophila sg1 Antigen Used and Evidence of Concurrent Reactivity to Other Atypical Pneumonia Agents, Page 1 of 2< Previous page Next page > /docserver/preview/fulltext/10.1128/9781555815660/9781555813901_Chap17-1.gif /docserver/preview/fulltext/10.1128/9781555815660/9781555813901_Chap17-2.gif
An outbreak of Legionnaires' disease (LD) occurred in August 2004 in Sweden in the town of Lidköping, by Lake Vänern. Two genotypes of Legionella pneumophila serogroup (sg) 1 were found by culture in patient samples, and one belonging to subgroup Benidorm (genotype A) and the other to subgroup Bellingham (genotype B). The indirect immunofluorescent antibody technique (IFAT) is still a standard method for antibody assay in patients with legionellosis, allowing for the screening of antibodies against several species and subtypes of Legionella. A serological study was therefore undertaken to assess the impact of the legionella outbreak on all pneumonia cases that occurred in the town. Patient samples were also tested for antibodies against other atypical pneumonia agents, i.e. Mycoplasma pneumoniae and Chlamydophila pneumoniae. Local experience in the laboratory using more than one subgroup of antigen had been shown earlier to increase sensitivity. Optimal sensitivity of immunofluorescent antibody tests in serological diagnosis of L. pneumphila sg 1 infections requires the use of an antigen subgroup that is in agreement with the antigenic setup of the epidemic strain.