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Chapter 19 : Duopath : a New Immunochromatographic Test for Simultaneous Identification of and Species

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Duopath : a New Immunochromatographic Test for Simultaneous Identification of and Species, Page 1 of 2

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Abstract:

Several molecular biological methods are increasingly being used for the identification of legionellae in man-made aquatic environments. Currently, the genus is known to include 51 species. Some of the species have been isolated only from environmental sources up to now, but it is generally accepted that all species may cause pneumonia, especially in immunocompromised persons. These huge numbers of spp. represent a very wide serological heterogeneity that can lead to unsatisfying sensitivity and specificity of serological identification tools. The specificity of Duopath was calculated by testing 50 bacterial strains isolated from water samples and grown on GVPC agar plates. The latex agglutination assay spp. recognizes seven of the most frequent non- species causing Legionnaires’ disease but not the wide range of legionellae found in water systems, which are also suspected to be pneumonia pathogens. In many countries, water or environmental samples have to be analyzed for the presence of spp. instead of only . Here, Duopath revealed a significant advantage over the latex assay and makes the phenotypic diagnostic gap significantly smaller. Therefore, Duopath can be considered a user-friendly, simple and reliable test for the simultaneous identification of and non-.

Citation: Helbig J, Lück P, Kunz B, Bubert A. 2006. Duopath : a New Immunochromatographic Test for Simultaneous Identification of and Species, p 73-75. In Cianciotto N, Kwaik Y, Edelstein P, Fields B, Geary D, Harrison T, Joseph C, Ratcliff R, Stout J, Swanson M (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555815660.ch19

Key Concept Ranking

Legionella pneumophila
0.73953795
Polymyxin B
0.51
0.73953795
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Figures

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FIGURE 1

Reading of Duopath (A) Sample is positive for spp. but not for ; (B) sample is positive for ; © sample is negative.

Citation: Helbig J, Lück P, Kunz B, Bubert A. 2006. Duopath : a New Immunochromatographic Test for Simultaneous Identification of and Species, p 73-75. In Cianciotto N, Kwaik Y, Edelstein P, Fields B, Geary D, Harrison T, Joseph C, Ratcliff R, Stout J, Swanson M (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555815660.ch19
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References

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1. Helbig, J. H.,, J. B. Kurtz,, M. Castellani Pastroris,, C. Pelaz, and, P. C. Lück. 1997. The antigenic lipopolysaccharide components of Legionella pneumophila recognized by monoclonal antibodies: possibilities and limitations for division of the species into serogroups. J. Clin. Microbiol. 35:28412845.
2. Joly, J. R.,, R. M. McKinney,, O. J. Tobin,, W. F. Bibb,, I. D. Watkins, and, D. Ramsay. 1986. Development of a standardized subgrouping scheme for Legionella pneumophila serogroup 1 using monoclonal antibodies. J. Clin. Microbiol. 23:768771.
3. Ratcliff, R. M.,, J. A. Lanser,, P. A. Manning, and, M. W. Heuzenroeder. 1998. Sequence-based classification scheme for the genus Legionella targeting the mip gene. J. Clin. Microbiol. 36:15601567.
4. Thacker, W. L.,, B. B. Plikaytis, and, H. W. Wilkinson. 1985. Identification of 22 Legionella species and 33 serogroups with the slide agglutination test. J. Clin. Microbiol. 21:779782.

Tables

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TABLE 1

Sensitivity of Duopath

Citation: Helbig J, Lück P, Kunz B, Bubert A. 2006. Duopath : a New Immunochromatographic Test for Simultaneous Identification of and Species, p 73-75. In Cianciotto N, Kwaik Y, Edelstein P, Fields B, Geary D, Harrison T, Joseph C, Ratcliff R, Stout J, Swanson M (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555815660.ch19

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