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Chapter 20 : Antigenic Diversity of a 19-Kilodalton Peptidoglycan-Associated Lipoprotein among Species Determined by Reactivity Patterns to Monoclonal Antibodies

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Antigenic Diversity of a 19-Kilodalton Peptidoglycan-Associated Lipoprotein among Species Determined by Reactivity Patterns to Monoclonal Antibodies, Page 1 of 2

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Abstract:

is an important cause of both community-acquired and nosocomial pneumonias. In this chapter, the authors produced 17 monoclonal antibodies (mAbs) against the recombinant PAL (rPAL) cloned from serogroup (sg) 1 and investigated antigenic diversity of the peptidoglycan-associated lipoprotein (PAL) proteins from soluble fractions of 16 species including 22 serogroups by mAb reactivity patterns in ELISA. Soluble antigens from strains ( sg 1, 3, 4, 5, and 6; ; ; ; ; ; ; ; sg 1 and 2; sg 1 and 2; sg 1) were prepared by the method of Berdal et al. The authors concluded that antigenic diversity of a species–common PAL protein is present among species and that developing diagnostic agents using a antigen, even if it is conserved, might be better for separately detecting species and non-pneumophila species.

Citation: Moon J, Yang J, Sim H, Park D, Sohn J, Lee H, Park M, Ja Kim M. 2006. Antigenic Diversity of a 19-Kilodalton Peptidoglycan-Associated Lipoprotein among Species Determined by Reactivity Patterns to Monoclonal Antibodies, p 76-78. In Cianciotto N, Kwaik Y, Edelstein P, Fields B, Geary D, Harrison T, Joseph C, Ratcliff R, Stout J, Swanson M (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555815660.ch20

Key Concept Ranking

Legionella pneumophila
0.8076923
Legionella
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0.8076923
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References

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1. Berdal, B. P.,, C. E. Farshy,, J. C. Farshy, and, J. C. Feeley. 1979. Detection of Legionella pneumophila antigen in urine by enzymelinked immunospecific assay. J. Clin. Microbiol. 9:575578.
2. Kim, M. J.,, J.W. Sohn,, D.W. Park,, S. C. Park, and, B. C. Chun. 2003. Characterization of a lipoprotein common to Legionella species as a urinary broad-spectrum antigen for diagnosis of Legionnaires’ disease. J. Clin. Microbiol. 41:29742979.
3. Kirby, B. D.,, K. M. Snyder,, R. D. Meyer, and, S. M. Finegold.1980. Legionnaires’ disease: report of sixty-five nosocomially acquired cases of review of the literature. Medicine 59:188205.
4. Stout, J. E. and V. L. Yu. 1997. Legionellosis. N. Engl. J. Med. 337:682687.

Tables

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TABLE 1

Reactivity patterns of the PAL antigens from soluble fractions of 11 species to 17 monoclonal antibodies by ELISA

Citation: Moon J, Yang J, Sim H, Park D, Sohn J, Lee H, Park M, Ja Kim M. 2006. Antigenic Diversity of a 19-Kilodalton Peptidoglycan-Associated Lipoprotein among Species Determined by Reactivity Patterns to Monoclonal Antibodies, p 76-78. In Cianciotto N, Kwaik Y, Edelstein P, Fields B, Geary D, Harrison T, Joseph C, Ratcliff R, Stout J, Swanson M (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555815660.ch20

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