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Chapter 33 : Persistence and Genotypic Stability of in a Potable-Water System in a Hotel over a 20-Month Period

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Persistence and Genotypic Stability of in a Potable-Water System in a Hotel over a 20-Month Period, Page 1 of 2

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Abstract:

This chapter determines the prevalence of spp. and identifies the chromosomal DNA subtypes and the genomic stability of these isolates during a 20-month follow-up period in the potable-water distribution system of a hotel. From June 2003 to March 2005, 110 water samples were collected from a hotel water supply comprising 11 sample collections of water. serogroup (sg.) 1 was isolated in 49 (44.55%) of the 110 samples tested. The cold water distal point was –positive throughout the whole study period. It should be pointed out that this distal point of cold water was an infrequently used point (roof-top tap) with stagnant water, which may explain the low chlorine levels (range, 0.05 to 0.95 mg of Cl per liter), the heating of water during warmer seasons and thus, colonization by throughout the study period. Environmental isolates of were genotypically characterized by pulsed-field gel electrophoresis. The number of colonized points was uniform throughout the 20-month study. All isolates analyzed showed the same chomosomal DNA genotype, which indicated the clone stability over the 20-month follow-up period.

Citation: García-Núñez M, Ferrer J, Ragull S, Junyent E, Sagristà E, Soler A, Sanchez I, Pedro-Botet M, Sopena N, Sabriá M. 2006. Persistence and Genotypic Stability of in a Potable-Water System in a Hotel over a 20-Month Period, p 124-127. In Cianciotto N, Kwaik Y, Edelstein P, Fields B, Geary D, Harrison T, Joseph C, Ratcliff R, Stout J, Swanson M (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555815660.ch33

Key Concept Ranking

Pulsed-Field Gel Electrophoresis
0.52025837
Legionella pneumophila
0.43563247
0.52025837
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Figures

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FIGURE 1

Pulsed-field gel electrophoresis. M, Market; 1, point 1 (June 2003); 2, point 2 (October 2003); 3, point 3 (January 2004); 4, point 4 (June 2004); 5, point 5 (June 2003);6, point 6 (October 2003); 7, point 7 (January 2004); 8, point 8 (June 2004);9, point 9 (March 2005); 10, point 4 (March 2005); 11, point 11 (March 2005).

Citation: García-Núñez M, Ferrer J, Ragull S, Junyent E, Sagristà E, Soler A, Sanchez I, Pedro-Botet M, Sopena N, Sabriá M. 2006. Persistence and Genotypic Stability of in a Potable-Water System in a Hotel over a 20-Month Period, p 124-127. In Cianciotto N, Kwaik Y, Edelstein P, Fields B, Geary D, Harrison T, Joseph C, Ratcliff R, Stout J, Swanson M (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555815660.ch33
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References

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1. Borella P.,, M. T. Montagna,, S. Stampi,, G. Stancanelli,, V. Romano-Spica,, M. Triasii,, I. Marchesi,, A. Bargellini,, D. Tato,, C. Napoli,, F. Zanetti,, E. Leoni,, M. Moro,, S. Scaltriti,, G. Ribera D’Alcala,, R. Satarpia, and, S. Boccia. 2005. Legionella contamination in hot water of Italian hotels. Appl. Environ. Microbiol. 71:58055813.
2. Garcia-Nuñez, M.,, S. Ragull,, M. L. Pedro-Botet,, N. Sopena,, E. Reynaga,, C. Rey-Joly,, J. Morera, and, M. Sabria. 2002. A single environmental clone of Legionella pneumophila serogroup 1 causing hospital-acquired Legionnaires’ disease over a 13-year period. 42th Interscience Conference on Antimicrobial Agents and Chemotherapy (ICAAC), San Diego.
3. ISO 11731:1998(E). 1998. Water Quality: Detection and Enumeration of Legionella.
4. McDade, J. E.,, C. C. Shephard,, D. W. Fraser,, T. R. Tsai,, M. A. Resudus, and, W. R. Dowdle, andthe Laboratory Investigation Team. 1997. Legionnaires’ disease. Isolation of a bacterium and demonstration of its role in other respiratory disease. N. Engl. J. Med. 297:11971203.
5. Real Decreto 865/2003, de 4 de Julio por el que se establecen los criterios higiénico-sanitarios para la prevención y control de la legionelosis (BOE 17, 18 julio 2003).
6. Sabria, M.,, M. Garcia-Núñez,, M. L. Pedro Botet,, N. Sopena,, J. M. Gimeno,, E. Reynaga,, J. Morera, and, C. Rey-Joly. 2001. Presence and chromosomal subtyping of Legionella species in potable water systems in 20 hospitals of Catalonia, Spain. Infect. Control Hosp. Epidemiol. 22:673676.
7. Zeybech, Z., and, A. Cotuk. 2002. Relationship between colonization of building water systems by Legionella pneumophila and environmental factors, p. 305308. In R. Marre et al. (ed.), Legionella. ASM Press, Washington, D.C.

Tables

Generic image for table
TABLE 1

Positive distal points of the hot potable distribution system on each collection round

Citation: García-Núñez M, Ferrer J, Ragull S, Junyent E, Sagristà E, Soler A, Sanchez I, Pedro-Botet M, Sopena N, Sabriá M. 2006. Persistence and Genotypic Stability of in a Potable-Water System in a Hotel over a 20-Month Period, p 124-127. In Cianciotto N, Kwaik Y, Edelstein P, Fields B, Geary D, Harrison T, Joseph C, Ratcliff R, Stout J, Swanson M (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555815660.ch33
Generic image for table
TABLE 2

Isolates typed by Sfi I pulsed field gel electrophoresis (PFGE)

Citation: García-Núñez M, Ferrer J, Ragull S, Junyent E, Sagristà E, Soler A, Sanchez I, Pedro-Botet M, Sopena N, Sabriá M. 2006. Persistence and Genotypic Stability of in a Potable-Water System in a Hotel over a 20-Month Period, p 124-127. In Cianciotto N, Kwaik Y, Edelstein P, Fields B, Geary D, Harrison T, Joseph C, Ratcliff R, Stout J, Swanson M (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555815660.ch33

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