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Chapter 38 : Sequence-Based Typing of as an Aid in Investigation of Hospital-Acquired Legionnaires’ Disease

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Sequence-Based Typing of as an Aid in Investigation of Hospital-Acquired Legionnaires’ Disease, Page 1 of 2

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Abstract:

The sequence-based typing (SBT) method was used to determine the allelic profiles of three sporadic clinical isolates as well as seven environmental isolates of serogroup 6, isolated at the Bacteriology Laboratory Unit of the Karolinska University Hospital in 2004. The SBT may prove to be an effective aid for the epidemiological investigation of nosocomial Legionnaires’ disease (LD). species, especially is a well-known cause of nosocomially acquired pneumonia, occurring frequently in immunocompromised subjects. The SBT method was used in a study to determine the allelic profiles of three serogroup 6 clinical isolates from suspected cases of nosocomial LD as well as seven environmental serogroup 6 isolates collected from drinking water sources in the vicinity of the patients being investigated. The aim of this study was to correlate the nosocomial infections to the environmental strain in the water systems of the hospital. Interestingly, the four control isolates collected from other hospitals in Sweden shared the same SBT profile, a finding that can be explained by the possible limited genomic heterogeneity within serogroup 6 compared to serogroup 1. Further typing of a larger number of serogroup 6 isolates is needed to validate this theory.

Citation: Fendukly F, Bernander S. 2006. Sequence-Based Typing of as an Aid in Investigation of Hospital-Acquired Legionnaires’ Disease, p 143-145. In Cianciotto N, Kwaik Y, Edelstein P, Fields B, Geary D, Harrison T, Joseph C, Ratcliff R, Stout J, Swanson M (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555815660.ch38

Key Concept Ranking

Legionella pneumophila
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Acute Lymphoblastic Leukemia
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References

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1. Bernander, S.,, K. Jacobson,, J. H. Helbig,, P. C. Luck, and, M.A. Lundholm. 2003. Hospital-associated outbreak of Legionnaires’ disease caused by Legionella pneumophila serogroup 1 is characterized by stable genetic fingerprinting but variable monoclonal antibody patterns. J. Clin. Microbiol. 41:25032508.
2. Bernander, S.,, K. Jacobson, and, M. Lundholm. 2004. A hospital-associated outbreak of Legionnaires’ disease caused by Legionella pneumophila serogroups 4 and 10 with a common genetic fingerprinting pattern. APMIS 112:210217.
3. Darelid, J.,, L. Bengtsson,, B. Gastrin,, H. Hallander,, S. Lofgren,, B. E. Malmvall, et al. 1994. An outbreak of Legionnaires’ disease in a Swedish hospital. Scand. J. Infect. Dis. 26:417425.
4. Gaia, V.,, F. N.,, B. Afshar,, P. C. Lück,, H. Meugnier,, J. Etienne,, R. Peduzzi, and, T. G. Harrison. 2005. A consensus sequence-based epidemiological typing scheme for clinical and environmental isolates of Legionella pneumophila, J. Clin. Microbiol., in press.
5. Fry, N. K.,, J. M. Bangsborg,, A. Bergmans,, S. Bernander,, J. Etienne,, L. Franzin, et al. 2002. Designation of the European Working Group on Legionella Infection (EWGLI) amplified fragment length polymorphism types of Legionella pneumophila serogroup 1 and results of intercentre proficiency testing using a standard protocol. Eur. J. Clin. Microbiol. Infect. Dis. 21:722728.
6. Gaia, V.,, N. K. Fry,, T. G. Harrison, and, R. Peduzzi. 2003. Sequence-based typing of Legionella pneumophila serogroup 1 offers the potential for true portability in legionellosis outbreak investigation. J. Clin. Microbiol. 41:29322939.
7. Gump, D. W., and, M. Keegan. 1986. Pulmonary infections due to Legionella in immunocompromised patients. Semin. Respir. Infect. 1:151159.
8. Helbig, J. H.,, J. B. Kurtz,, M. C. Pastoris,, C. Pelaz, and, P. C. Luck. 1997. Antigenic lipopolysaccharide components of Legionella pneumophila recognized by monoclonal antibodies: possibilities and limitations for division of the species into serogroups. J. Clin. Microbiol. 35:28412845.
9. Isenberg, H. D. (ed.) Clinical Microbiology Procedures Handbook. 1992;1:1. 12.2630.
10. Wilczek, H.,, I. Kallings,, B. Nystrom,, G. Tyden, and, C. G. Groth. 1985. Experience with 5 cases of Legionnaires’ disease at the Department of Transplantation Surgery, Huddinge Hospital, Stockholm, Sweden. Scand. J. Urol. Nephrol. Suppl. 92:6769.

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