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Chapter 41 : Epidemiological Typing of in the Absence of Isolates

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Epidemiological Typing of in the Absence of Isolates, Page 1 of 2

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Abstract:

The recently described sequence-based typing (SBT) scheme for epidemiological typing of offers many advantages over other methods. However, clinical and environmental isolates are not always available to allow epidemiological typing to be performed. For this reason the authors have sought to apply the SBT technique directly to clinical and environmental samples where isolates are not available or before they become available. This has the potential to allow more rapid determination of typing data or the possibility of obtaining such data when there is no alternative, i.e., in the absence of isolates. One aim was to determine if this direct approach was feasible. This was established using environmental concentrates, together with environmental and clinical isolates, obtained during the investigation of the largest United Kingdom outbreak to date, in Barrow-in-Furness in 2002. The case had antibody levels consistent with infection by , but no isolate was obtained. In 2005 a cluster of three cases was reported from the same complex. Respiratory samples from two suspected cases (cases 1 and 2) were obtained, but was only isolated from one (case 1). PCR analysis of the extract from case 2 was also undertaken using an in-house –specific real-time PCR assay targeting the macrophage infectivity potentiator (mip) gene. SBT was performed on DNA extracted from both the isolate (case 1) and the clinical sample (case 2). Direct SBT was also successfully demonstrated using clinical samples including respiratory and postmortem specimens.

Citation: K. Fry N, Afshar B, Wewalka G, G. Harrison T. 2006. Epidemiological Typing of in the Absence of Isolates, p 152-155. In Cianciotto N, Kwaik Y, Edelstein P, Fields B, Geary D, Harrison T, Joseph C, Ratcliff R, Stout J, Swanson M (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555815660.ch41

Key Concept Ranking

Real-Time PCR
0.8409091
Legionella pneumophila
0.6363636
0.8409091
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References

/content/book/10.1128/9781555815660.ch41
1. Gaia, V.,, N. K. Fry,, B. Afshar,, P. C. Lück,, H. Meugnier,, J. Etienne,, R. Peduzzi, and, T. G. Harrison. 2005. Consensus sequence-based scheme for epidemiological typing of clinical and environmental isolates of Legionella pneumophila. J. Clin. Microbiol. 43:20472052.
2. Gaia, V.,, N. K. Fry,, T. G. Harrison, and, R. Peduzzi. 2003. Sequence-based typing of Legionella pneumophila serogroup 1 offers the potential for true portability in legionellosis outbreak investigation. J. Clin. Microbiol. 41:29322939.

Tables

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TABLE 1

Result of investigation of legionellosis by PCR and SBT in (a) a holiday camp in Hampshire, UK, (b) an Austrian campsite, (c) a nosocomial case in an English hospital, (d) and another case in the same hospital as (c)

Citation: K. Fry N, Afshar B, Wewalka G, G. Harrison T. 2006. Epidemiological Typing of in the Absence of Isolates, p 152-155. In Cianciotto N, Kwaik Y, Edelstein P, Fields B, Geary D, Harrison T, Joseph C, Ratcliff R, Stout J, Swanson M (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555815660.ch41

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