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Chapter 48 : Subcellular Localization of the Dot/Icm Type IV Secretion Proteins

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Subcellular Localization of the Dot/Icm Type IV Secretion Proteins, Page 1 of 2

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Abstract:

is the causative agent of Legionnaires’ disease, a fatal form of pneumonia in elderly and immunocompromised people. replicates inside host cells by using a type IV secretion system (T4SS) encoded by the genes. The genes are essential for intracellular multiplication of . To determine the localization of the T4SS apparatus, the authors initially investigated the distribution of one Dot protein, DotF. DotF, predicted to localize to the inner membrane by hydrophilicity analysis, has been shown to interact with secreted substrates via a bacterial two-hybrid screen. Quantitative analysis showed that more than 95% of cells had at least one focus of fluorescence at a pole. The majority of the cells showed bipolar DotF staining. The polar staining was specific to DotF because it was not apparent in a strain lacking the gene. VirB T4SS has been proposed to provide a selective advantage since this plant pathogen attaches to its host cell in a polar manner, thereby allowing intimate contact between the secretion system and the plant cell. Based on the observations, and the work on the VirB system, it is possible that polar localization is a conserved feature of all adapted type IV secretion systems. Alternatively, as in the case of adapted conjugation systems and IcsA, there may be a selective advantage or molecular requirement for the proteins to be localized to the bacterial pole.

Citation: Cheol Jeong K, D. Vincent C, Buford E, P. Vogel J. 2006. Subcellular Localization of the Dot/Icm Type IV Secretion Proteins, p 192-194. In Cianciotto N, Kwaik Y, Edelstein P, Fields B, Geary D, Harrison T, Joseph C, Ratcliff R, Stout J, Swanson M (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555815660.ch48

Key Concept Ranking

Type IV Secretion Systems
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Bacterial Proteins
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Figures

Image of FIGURE 1
FIGURE 1

Intracellular localization of DotF in wild type (WT) and a Δ mutant. Cells were grown to stationary phase and analyzed by fluorescence microscopy. Cells were detected by staining with the DNA stain DAPI. Foci of DotF proteins were detected using an antibody that specifically recognizes DotF.

Citation: Cheol Jeong K, D. Vincent C, Buford E, P. Vogel J. 2006. Subcellular Localization of the Dot/Icm Type IV Secretion Proteins, p 192-194. In Cianciotto N, Kwaik Y, Edelstein P, Fields B, Geary D, Harrison T, Joseph C, Ratcliff R, Stout J, Swanson M (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555815660.ch48
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References

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1. Bardill, J. P.,, J. L. Miller, and, J. P. Vogel. 2005. IcmS-dependent translocation of SdeA into macrophages by the Legionella pneumophila type IV secretion system. Mol. Microbiol. 56:90103.
2. Christie, P. J.,, K. Atmakuri,, V. Krishnamoor-thy,, S. Jakubowski, and, E. Cascales. 2005. Biogenesis, architecture, and function of bacterial type IV secretion systems. Annu. Rev. Microbiol. 59:451485.
3. Conover, G. M.,, I. Derre,, J. P. Vogel, and, R. R. Isberg. 2003. The Legionella pneumophila LidA protein: a translocated substrate of the Dot/ Icm system associated with maintenance of bacterial integrity. Mol. Microbiol. 48:305321.
4. Janakiraman, A., and, M. B. Goldberg. 2004. Evidence for polar positional information independent of cell division and nucleoid occlusion. Proc. Natl. Acad. Sci. USA 101:835840.
5. Komano, T.,, T. Yoshida,, K. Narahara, and, N. Furuya. 2000. The transfer region of IncI1 plas-mid R64: similarities between R64 tra and Legionella icm/dot genes. Mol. Microbiol. 35:13481359.
6. Luo, Z. Q., and, R. R. Isberg. 2004. Multiple substrates of the Legionella pneumophila Dot/Icm system identified by interbacterial protein transfer. Proc. Natl. Acad. Sci. USA 101:841846.
7. Segal, G.,, M. Feldman, and, T. Zusman. 2005. The Icm/Dot type-IV secretion systems of Legionella pneumophila and Coxiella burnetii. FEMS Microbiol. Rev. 29:6581.
8. Segal, G.,, M. Purcell, and, H. A. Shuman. 1998. Host cell killing and bacterial conjugation require overlapping sets of genes within a 22-kb region of the Legionella pneumophila genome. Proc. Natl. Acad. Sci. USA 95:16691674.
9. Sexton, J. A., and, J. P. Vogel. 2002. Type IVB secretion by intracellular pathogens. Traffic 3:178185.
10. Vogel, J. P.,, H. L. Andrews,, S. K. Wong, and, R. R. Isberg. 1998. Conjugative transfer by the virulence system of Legionella pneumophila. Science 279:873876.

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