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Chapter 50 : Loss of a Patatin-Like Phospholipase A Causes Reduced Infectivity of in Amoeba and Machrophage Infection Models

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Loss of a Patatin-Like Phospholipase A Causes Reduced Infectivity of in Amoeba and Machrophage Infection Models, Page 1 of 2

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Abstract:

possesses a large variety of lipolytic enzyme activities that affect phospholipids. In addition to the phospholipid-degrading phospholipase A (PLA) and lysophospholipase A (LPLA) activities, glycerophospholipid:cholesterol acyltransferase (GCAT) activity has been described in this chapter. First, the authors were interested in whether all of the 11 PLP genes are expressed in Philadelphia-1 during growth in laboratory media. In order to assess expression of the PLP genes, mRNA was isolated at four growth phases (early logarithmic, mid-logarithmic, late logarithmic, and early stationary) in standard laboratory media and subsequently used for reverse transcriptase PCR with Philadelphia-1 PLP gene-specific primers. wild type and the mutants were grown to late exponential phase, and cell lysates and culture supernatants of the bacteria were tested for PLA, LPLA, and lipase activities. Importantly, in coinfection assays with amoebae and macrophages, the mutant strains were severely impaired for intracellular replication. Thus, PatA/VipD is a new type IVB secreted phospholipase of with essential importance during host cell infection.

Citation: Aurass P, Banerji S, Flieger A. 2006. Loss of a Patatin-Like Phospholipase A Causes Reduced Infectivity of in Amoeba and Machrophage Infection Models, p 199-202. In Cianciotto N, Kwaik Y, Edelstein P, Fields B, Geary D, Harrison T, Joseph C, Ratcliff R, Stout J, Swanson M (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555815660.ch50

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Type IVB Secretion System
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Figures

Image of FIGURE 1
FIGURE 1

Expression of PLP genes during growth in laboratory media. Expression of the 11 Philadelphia-1 PLP genes, to , was examined during exponential and late logarithmic growth phase in BYE broth. RNA was isolated and reverse tran-scriptase PCR was performed. The results are representative of two independent experiments.

Citation: Aurass P, Banerji S, Flieger A. 2006. Loss of a Patatin-Like Phospholipase A Causes Reduced Infectivity of in Amoeba and Machrophage Infection Models, p 199-202. In Cianciotto N, Kwaik Y, Edelstein P, Fields B, Geary D, Harrison T, Joseph C, Ratcliff R, Stout J, Swanson M (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555815660.ch50
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Image of FIGURE 2
FIGURE 2

shows LPLA activity when expressed in Release of free fatty acids from monopalmitoyllysophosphatidylglycerol, monopalmitoylly-sophosphatidylcholine, monopalmitoyllysophosphatidylethanolamine, 1-monopalmitoyl-glycerol, dipalmitoylphosphatidylglycerol, and dipalmitoylphosphatidylcholine by three times concentrated cell lysates of DH5α containing the pBCKS vector in comparison to DH5α containing its derivative pPA3 with carrying pKH192, harboring the PLA and LPLA gene ( ), was used as a positive control. Free fatty acids were quantified after 24 h incubation at 37°C. Data are means + /— standard deviation of three experiments and are shown as the difference between fatty acids released by bacterial lysate and by negative control (40 mM Tris-HCl buffer).

Citation: Aurass P, Banerji S, Flieger A. 2006. Loss of a Patatin-Like Phospholipase A Causes Reduced Infectivity of in Amoeba and Machrophage Infection Models, p 199-202. In Cianciotto N, Kwaik Y, Edelstein P, Fields B, Geary D, Harrison T, Joseph C, Ratcliff R, Stout J, Swanson M (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555815660.ch50
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References

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8. Phillips, R. M.,, D. A. Six,, E. A. Dennis, and, P. Ghosh. 2003. In vivo phospholipase activity of the Pseudomonas aeruginosa cytotoxin ExoU and protection of mammalian cells with phospholi-pase A2 inhibitors. J. Biol. Chem. 278:4132641332.
9. Sato, H.,, D. W. Frank,, C. J. Hillard, et al. 2003. The mechanism of action of the Pseudomo-nas aeruginosa-encoded type III cytotoxin, ExoU. EMBO J. 22:29592969.
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