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Chapter 53 : The Type II Protein Secretion System of Is Important for Growth in Iron-Rich Media and Survival in Tap Water at Low Temperatures

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The Type II Protein Secretion System of Is Important for Growth in Iron-Rich Media and Survival in Tap Water at Low Temperatures, Page 1 of 2

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Abstract:

The type II protein secretion system of is important for bacterial survival in protozoa, macrophages, and the lungs of A/J mice. Presently, is known to express both type II and type IV secretion systems. Type II secretion () mutants of grow normally in bacteriologic media at 30 to 37°C. The low-temperature defect of mutants of serogroup 1 strain 130b was partially due to the presence of 0.25 g/liter ferric pyrophosphate in the growth media, since bacterial growth on agar plates at 25°C improved significantly when that supplement was omitted. The inhibitory effect of iron was specific to the mutant and to low-temperature growth, since the growth of wild type at 25°C did not change with variations in the amount of supplemental iron, and the growth of the mutant was not negatively influenced by iron supplementation when incubated at 37°C. In the environment, is found in freshwater and man-made water systems. In sum, the results presented indicate that the type II secretion system is not only important for growth at 35 to 37°C in host cells but is also critical for extracellular growth and survival at lower temperatures, including iron-rich bacteriological media and environmental water samples. Thus, type II secretion appears to play a central and multifaceted role in the natural history of Legionnaires’ disease.

Citation: A. Söderberg M, P. Cianciotto N. 2006. The Type II Protein Secretion System of Is Important for Growth in Iron-Rich Media and Survival in Tap Water at Low Temperatures, p 214-216. In Cianciotto N, Kwaik Y, Edelstein P, Fields B, Geary D, Harrison T, Joseph C, Ratcliff R, Stout J, Swanson M (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555815660.ch53

Key Concept Ranking

Type II Secretion System
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Type IV Secretion Systems
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Figures

Image of FIGURE 1
FIGURE 1

The effect of iron on the low-temperature growth of wild type and mutants. Wild-type 130b and an mutant were grown on standard BCYE agar for 3 days at 37°C, and then the bacteria were resuspended to the same optical density, serially diluted, and plated onto BCYE agar containing or not containing the traditional 0.25 g/liter ferric pyrophosphate supplement. The inoculated plates were then incubated at 37 or 25°C, and growth was observed over the next 12 days. The presented image is the bacterial growth that resulted from plating the 10 dilution on agar incubated at 25°C These results are representative of two independent experiments.

Citation: A. Söderberg M, P. Cianciotto N. 2006. The Type II Protein Secretion System of Is Important for Growth in Iron-Rich Media and Survival in Tap Water at Low Temperatures, p 214-216. In Cianciotto N, Kwaik Y, Edelstein P, Fields B, Geary D, Harrison T, Joseph C, Ratcliff R, Stout J, Swanson M (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555815660.ch53
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Image of FIGURE 2
FIGURE 2

The survival of wild type and mutant in tap water at different temperatures. Wild-type 130b and mutant bacteria were grown in BYE broth to log phase at 37°C and then centrifuged. The resultant bacterial pellets were resuspended in filter-sterilized tap water, recentrifuged, and again resuspended in tap water in order to remove traces of BYE broth. Then, equal numbers of wild type and mutant bacteria were inoculated into flasks containing 50 ml of filtered sterilized tap water, and the cultures were incubated at 37°C (A) or 17°C (B) with shaking. Aliquots were removed at various times, and CFU determinations were made by plating serial dilutions on BCYE agar. The results presented are the means and standard deviations from duplicate samples and are representative of two independent experiments.

Citation: A. Söderberg M, P. Cianciotto N. 2006. The Type II Protein Secretion System of Is Important for Growth in Iron-Rich Media and Survival in Tap Water at Low Temperatures, p 214-216. In Cianciotto N, Kwaik Y, Edelstein P, Fields B, Geary D, Harrison T, Joseph C, Ratcliff R, Stout J, Swanson M (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555815660.ch53
Permissions and Reprints Request Permissions
Download as Powerpoint

References

/content/book/10.1128/9781555815660.ch53
1. Cianciotto, N. P. 2005. Type II secretion: a protein secretion system for all seasons. Trends Microbiol. 13:581588.
2. Rossier, O.,, S. R. Starkenburg, and, N. P. Cianciotto. 2004. Legionella pneumophila type II protein secretion promotes virulence in the A/J mouse model of Legionnaires’ disease pneumonia. Infect. Immun. 72:310321.
3. Söderberg, M. A.,, O. Rossier, and, N. P. Cianciotto. 2004. The type II protein secretion system of Legionella pneumophila promotes growth at low temperatures. J. Bacteriol. 186:37123720.
4. Winterbourn, C. C. 1995. Toxicity of iron and hydrogen peroxide: the Fenton reaction. Toxicol. Lett. 82/83: 969974.

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