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Chapter 55 : Identification of Target Proteins of the Lss Secretion System of Corby

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Identification of Target Proteins of the Lss Secretion System of Corby, Page 1 of 2

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Abstract:

This chapter describes a first putative type I secretion system (Lss) of which is encoded by the locus. In order to identify substrates of this Lss secretion system, the authors performed comparative two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) analysis of extracellular proteins of Corby wild type, Corby lssB mutant, and complemented lssB mutant strain. In this study it was shown that the identified VirK protein of is secreted in an LssB- but not a TatC-dependent manner. Referring to the results, it is implied that the Lss secretion system is also linked to the Lsp type II secretion system in . It is speculated that VirK and p14 are secreted via a concerted action of the Lss and the Lsp system which includes cleavage of the signal sequences during secretion. In and VirK is associated with the vir-regulon, and it was postulated that VirK should have a function in the interaction of the bacteria with the host cells. Therefore, the authors started to generate a mutant strain of Corby and an antibody against VirK to further characterize the expression, secretion, and function of VirK of .

Citation: Albert C, Jacobi S, De Buck E, Lammertyn E, Heuner K. 2006. Identification of Target Proteins of the Lss Secretion System of Corby, p 221-223. In Cianciotto N, Kwaik Y, Edelstein P, Fields B, Geary D, Harrison T, Joseph C, Ratcliff R, Stout J, Swanson M (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555815660.ch55

Key Concept Ranking

Type II Secretion System
0.5830467
Type IV Secretion Systems
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Type I Secretion System
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0.5830467
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Figures

Image of FIGURE 1
FIGURE 1

Genomic organization and putative protein function of the locus of . The first line of arrows indicates genes present in the locus. Putative proteins and protein motifs are shown below. DedA, DedA-related protein family; PAP2, type 2 phosphatidic acid phosphatase superfamily.

Citation: Albert C, Jacobi S, De Buck E, Lammertyn E, Heuner K. 2006. Identification of Target Proteins of the Lss Secretion System of Corby, p 221-223. In Cianciotto N, Kwaik Y, Edelstein P, Fields B, Geary D, Harrison T, Joseph C, Ratcliff R, Stout J, Swanson M (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555815660.ch55
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Image of FIGURE 2
FIGURE 2

(A) Cutout of comparative analysis of extracellular proteins of Corby wild type and Corby mutant. Proteins of interest were analyzed by mass spectrometry and N-terminal sequencing. (B) Signal sequences of p13 and p14. Arrows mark the putative sites of processing. Amino acids identified by N-terminal sequencing are in bold.

Citation: Albert C, Jacobi S, De Buck E, Lammertyn E, Heuner K. 2006. Identification of Target Proteins of the Lss Secretion System of Corby, p 221-223. In Cianciotto N, Kwaik Y, Edelstein P, Fields B, Geary D, Harrison T, Joseph C, Ratcliff R, Stout J, Swanson M (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555815660.ch55
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References

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