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Chapter 69 : Induction of Apoptosis during Intracellular Replication of in the Lungs of Mice

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Induction of Apoptosis during Intracellular Replication of in the Lungs of Mice, Page 1 of 2

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Abstract:

has been shown to induce apoptosis within macrophages, monocytic cell lines, and alveolar epithelial cells. This chapter addresses the Dot/Icm-mediated induction of apoptosis in genetically susceptible A/J mice during infection. In an experiment A/J and BALB/c mice were intratracheally inoculated with 10 cfu of AA100- green fluorescent protein (GFP) or the -GFP mutant. At different time points after infection (2, 24, 48, and 72 h) mice were sacrificed and the lungs were removed. It has been recently shown that robust activation of caspase-3 is exhibited throughout the early and exponential intracellular replication of , but apoptosis is delayed and is not triggered in the infected cells until the late stages of infection, concomitant with termination of intracellular replication. AA100 induces apoptosis in vivo during the late stages of Legionnaires’ disease in experimental animals. Genetically susceptible A/J mice are susceptible to the Dot/Icm-mediated induction of apoptosis. Apoptosis is triggered at the late stages of infection, concomitant with termination of intracellular replication. BALB/c mice are resistant to infection of AA100 as well as induction of apoptosis.

Citation: M. Santic, M. Molmeret, S. Jones, R. Asare, A. Abu-Zant, M. Doric, Y. Abu Kwaik. 2006. Induction of Apoptosis during Intracellular Replication of in the Lungs of Mice, p 283-287. In Cianciotto N, Kwaik Y, Edelstein P, Fields B, Geary D, Harrison T, Joseph C, Ratcliff R, Stout J, Swanson M (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555815660.ch69

Key Concept Ranking

Type IV Secretion Systems
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Confocal Laser Scanning Microscopy
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Figures

Image of FIGURE 1
FIGURE 1

Growth kinetics of AA100 and its isogenic mutant in the lungs of A/J () and BALB/c () mice. Animals were inoculated with AA100 or the mutant. At specific time points, the mice were sacrificed and the number of bacteria was determined in the lungs at 2, 24, 48, and 72 h after infection. The error bars represent standard deviations of five mice per group, and results shown are representative of three independent experiments.

Citation: M. Santic, M. Molmeret, S. Jones, R. Asare, A. Abu-Zant, M. Doric, Y. Abu Kwaik. 2006. Induction of Apoptosis during Intracellular Replication of in the Lungs of Mice, p 283-287. In Cianciotto N, Kwaik Y, Edelstein P, Fields B, Geary D, Harrison T, Joseph C, Ratcliff R, Stout J, Swanson M (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555815660.ch69
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Image of FIGURE 2
FIGURE 2

Representative laser scanning confocal microscopy images of lung tissue of A/J mice infected with AA100 () and mutant (). A/J mice were infected with the AA100-GFP and the -GFP mutant with 10 cfu/mouse. At 2, 24, and 48 h after infection lungs were processed to be sectioned and labeled for TUNEL. Lung tissue of uninfected A/J mice, DNase-treated, and untreated lung tissues were used as controls. Apoptotic cells were labeled using TUNEL (black), and the bacteria are shown in gray (GFP). The experiments were done in triplicate using 5 mice for each time point, and the images are representative of 20 microscopic fields from each animal. The results are representative of three independent experiments.

Citation: M. Santic, M. Molmeret, S. Jones, R. Asare, A. Abu-Zant, M. Doric, Y. Abu Kwaik. 2006. Induction of Apoptosis during Intracellular Replication of in the Lungs of Mice, p 283-287. In Cianciotto N, Kwaik Y, Edelstein P, Fields B, Geary D, Harrison T, Joseph C, Ratcliff R, Stout J, Swanson M (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555815660.ch69
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Image of FIGURE 3
FIGURE 3

Failure of to induce pulmonary apoptosis in resistant BALB/c mice. Representative laser scanning confocal microscopy images of lung tissue of BALB/c mice infected with the AA100. BALB/c mice were infected with AA100-GFP with 10 cfu/mouse. At 2, 24, and 48 h after infection, lungs were processed to be sectioned and labeled for TUNEL. Lung tissue of uninfected BALB/c mice, DNase-treated, and untreated lung tissues were used as controls. Apoptotic cells were labeled using TUNEL (black), and the bacteria are shown in gray (GFP). The experiments were done in triplicate using 5 mice for each time point, and the images are representative of 20 microscopic fields from each animal. The results are representative of three independent experiments.

Citation: M. Santic, M. Molmeret, S. Jones, R. Asare, A. Abu-Zant, M. Doric, Y. Abu Kwaik. 2006. Induction of Apoptosis during Intracellular Replication of in the Lungs of Mice, p 283-287. In Cianciotto N, Kwaik Y, Edelstein P, Fields B, Geary D, Harrison T, Joseph C, Ratcliff R, Stout J, Swanson M (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555815660.ch69
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Download as Powerpoint

References

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