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Chapter 80 : Identification of Genes under Transcriptional Control of LpnR Regulatory Proteins

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Identification of Genes under Transcriptional Control of LpnR Regulatory Proteins, Page 1 of 2

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Abstract:

In a search for Philadelphia proteins with sequence similarity to members of the LuxR family of transcriptional regulators, three novel proteins were identified. These were designated LpnR1 (lpg 2557), LpnR2 (lpg 1946), and LpnR3 (lpg 1448), and although these proteins were not quorum-sensing related, they act as transcriptional regulators. For the malate dehydrogenase gene (), a semiquantitative RT-PCR analysis was performed to check transcriptional control by LpnR3. Total RNA was isolated from , and ΔlpnR3 was grown to exponential and stationary growth phase in culture broth. RT-PCR with -specific primers (5' -atggatccggcaatcaaagtaac-3' and 5' -atggatcctagaacatatggttc-3') was performed on 100 ng of total RNA. Activation of transcription of two other genes, i.e., the triacyl glycerol lipase and the acetoacetate decarboxylase genes, on the other hand, is dependent on LpnR3, but rather indirectly, or requires an additional factor.

Citation: E. Lammertyn, L. Maes, E. De Buck, I. Lebeau, J. Anné. 2006. Identification of Genes under Transcriptional Control of LpnR Regulatory Proteins, p 333-335. In Cianciotto N, Kwaik Y, Edelstein P, Fields B, Geary D, Harrison T, Joseph C, Ratcliff R, Stout J, Swanson M (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555815660.ch80

Key Concept Ranking

Malate Dehydrogenase
0.6043536
Chromosomal DNA
0.6
Legionella pneumophila
0.5725455
0.6043536
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Figures

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FIGURE 1

RT-PCR analysis of the A gene. RNA was isolated from wild type and Δ3, and RT-PCR with A-specific primers was performed on 100 ng total RNA. Amplification fragments were analyzed after 30 cycli and loaded on agarose gel in twofold dilutions. Control reactions on the 16S rRNA resulted in a signal of equal intensity for wild-type and Δ3 samples (data not shown). Controls for the presence of traces of DNA in the samples were negative.

Citation: E. Lammertyn, L. Maes, E. De Buck, I. Lebeau, J. Anné. 2006. Identification of Genes under Transcriptional Control of LpnR Regulatory Proteins, p 333-335. In Cianciotto N, Kwaik Y, Edelstein P, Fields B, Geary D, Harrison T, Joseph C, Ratcliff R, Stout J, Swanson M (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555815660.ch80
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Image of FIGURE 2
FIGURE 2

Schematic presentation of the two-plasmid system used. cells containing both plas-mids were grown in the presence or absence of IPTG.

Citation: E. Lammertyn, L. Maes, E. De Buck, I. Lebeau, J. Anné. 2006. Identification of Genes under Transcriptional Control of LpnR Regulatory Proteins, p 333-335. In Cianciotto N, Kwaik Y, Edelstein P, Fields B, Geary D, Harrison T, Joseph C, Ratcliff R, Stout J, Swanson M (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555815660.ch80
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References

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1. Geukens, N.,, E. Lammertyn,, L. Van Mel-laert,, S. Schacht,, K. Schaerlaekens,, V. Parro,, S. Bron,, Y. Engelborghs,, R. P. Mellado, and, J. Anné. 2001. Membrane topology of the Strepto-myces lividans type I signal peptidases. J. Bacteriol. 183:47524760.
2. Lebeau, I.,, E. Lammertyn,, E. De Buck,, L. Maes,, N. Geukens,, L. Van Mellaert, and, J. Anné. 2004. Novel transcriptional regulators of Legionella pneumophila that affect replication in Acanthamoeba castellanii. Arch. Microbiol. 181:362370.
3. Lebeau, I.,, E. Lammertyn,, E. De Buck,, L. Maes,, N. Geukens,, L. Van Mellaert,, L. Arck-ens,, J. Annè, and, S. Clerens. 2005. First pro-teomic analysis of Legionella pneumophila based on its developing genome sequence. Res. Microbiol. 156:119129.

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