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Chapter 83 : Analysis of Gene Expression in during Axenic Growth and Infection

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Analysis of Gene Expression in during Axenic Growth and Infection, Page 1 of 2

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Abstract:

To understand the genetic basis of the ability of to adapt to a wide variety of different environmental settings, the authors have initiated a long-term genome-wide gene expression profiling study of wild-type strains Philadelphia 1 and JR32 and several mutants during their growth in rich axenic media as well as at different stages of using whole-genome microarrays. The gene expression measurements for key operons were independently confirmed by real-time PCR. Gene expression profiles of wild type and mutants were compared during growth in rich axenic media. About 30% of the bacterial genome was found to be expressed under any single condition tested in the axenic growth experiments. Infection was initiated by centrifugation of the bacteria onto the adherent amoebae. About 500 genes were found to be expressed during the internalization and early stages of infection; 78 of them were also expressed during growth in liquid culture. This chapter provides a comparison of expressed gene complements between axenic growth and infection and some gene ontology (GO) functional gene categories expressed during the infection. Further studies should provide a more detailed picture of coordinated gene expression during infection as well as the molecular events that accompany it.

Citation: Pampou S, Morozova I, Hilbert D, de Felipe K, Morozov P, J. Russo J, A. Shuman H, Kalachikov S. 2006. Analysis of Gene Expression in during Axenic Growth and Infection, p 343-346. In Cianciotto N, Kwaik Y, Edelstein P, Fields B, Geary D, Harrison T, Joseph C, Ratcliff R, Stout J, Swanson M (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555815660.ch83

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Horizontal Gene Transfer
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FIGURE 1

Comparison of gene complements differentially expressed during axenic growth and infection. While GO functional categories are represented in relatively equal proportions between axenic growth (a) and earlier stages of the infection (b), the actual number of common genes between the two groups does not exceed 20% (c).

Citation: Pampou S, Morozova I, Hilbert D, de Felipe K, Morozov P, J. Russo J, A. Shuman H, Kalachikov S. 2006. Analysis of Gene Expression in during Axenic Growth and Infection, p 343-346. In Cianciotto N, Kwaik Y, Edelstein P, Fields B, Geary D, Harrison T, Joseph C, Ratcliff R, Stout J, Swanson M (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555815660.ch83
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References

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