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Chapter 86 : Genetic Diversity of

Authors: C. Cazalet1, S. Jarraud2, Y. Ghavi-Helm3, J. Allignet4, F. Kunst5, J. Etienne6, P. Glaser7, C. Buchrieser8
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Affiliations: 1: Unité de Génomique des Microorganismes Pathogènes, Institut Pasteur, 28, rue du Dr. Roux, 75724 Paris Cedex 15, France; 2: Centre National de Référence des , Laboratoire de Bactériologie INSERM E-0230, Faculté de Médecine, IFR 62, 69372, Lyon Cedex 08; 3: Unité de Génomique des Microorganismes Pathogènes, Institut Pasteur, 28, rue du Dr. Roux, 75724 Paris Cedex 15, France; 4: Institut Pasteur, Plate-forme puces à ADN, CNR/Sante publique, 28, rue du Dr. Roux, 75724 Paris Cedex 15, France; 5: Unité de Génomique des Microorganismes Pathogènes, Institut Pasteur, 28, rue du Dr. Roux, 75724 Paris Cedex 15, France; 6: Centre National de Référence des , Laboratoire de Bactériologie INSERM E-0230, Faculté de Médecine, IFR 62, 69372, Lyon Cedex 08; 7: Unité de Génomique des Microorganismes Pathogènes, Institut Pasteur, 28, rue du Dr. Roux, 75724 Paris Cedex 15, France; 8: Unité de Génomique des Microorganismes Pathogènes, Institut Pasteur, 28, rue du Dr. Roux, 75724 Paris Cedex 15, France
Content Type: Monograph
Publication Year: 2006

Category: Bacterial Pathogenesis

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Genetic Diversity of , Page 1 of 2

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Abstract:

In order to explore the genetic diversity in a large number of strains, a biodiversity array was designed containing probes (internal PCR products) specific for all those genes that are variable among the three strains as well as of conserved ones that have known or putative implication in virulence. The gene content of 180 isolates, 165 strains, and 25 representatives of species other than isolated from the environment during epidemics or from sporadic cases were analyzed by DNA-DNA hybridization using the biodiversity array. serogroup 1 is largely overrepresented among human legionellosis cases, suggesting that virulence differences exist among different serogroups and other species. In order to address this question and to try to find a possible link between virulence differences and a specific pattern of virulence genes, the authors analyzed the distribution of these factors among 180 strains. This study also reports for the first time the distribution of the eukaryotic-like genes identified during sequence analysis among . In order to better understand plasticity and evolution in the authors used hierarchical clustering to analyze the hybridization patterns obtained. Despite the high genome plasticity observed, hierarchical clustering of the hybridization results of the genes selected for the biodiversity array correlates with results of sequence typing and therefore with phylogeny. The biodiversity array allowed subdivision of the otherwise indistinguishable group of Paris strains.

Citation: Cazalet C, Jarraud S, Ghavi-Helm Y, Allignet J, Kunst F, Etienne J, Glaser P, Buchrieser C. 2006. Genetic Diversity of , p 355-358. In Cianciotto N, Kwaik Y, Edelstein P, Fields B, Geary D, Harrison T, Joseph C, Ratcliff R, Stout J, Swanson M (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555815660.ch86

Key Concept Ranking

Type II Secretion System
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Type IV Secretion Systems
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Mobile Genetic Elements
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Genetic Diversity of Legionella pneumophila
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Citation: Cazalet C, Jarraud S, Ghavi-Helm Y, Allignet J, Kunst F, Etienne J, Glaser P, Buchrieser C. 2006. Genetic Diversity of , p 355-358. In Cianciotto N, Kwaik Y, Edelstein P, Fields B, Geary D, Harrison T, Joseph C, Ratcliff R, Stout J, Swanson M (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555815660.ch86
/content/10.1128/9781555815660.ch86.ch86fig01
FIGURE 1
Schematic representation of the integrative multicopy plasmid carrying the type IV system and comparison of this region in the three strains. The scale on the right indicates the G+C content values in this region. The black curve indicates variations in G+C content along this region in strain Lens. Dark grey bars, homologous regions in the three genomes; light grey arrow, genes predicted in this region. Arrows and numbers indicate the insertion position on the chromosome of each strain.
10.1128/9781555815660/f0357-01_thmb.gif
10.1128/9781555815660/f0357-01.gif
Image of FIGURE 1
FIGURE 1

Schematic representation of the integrative multicopy plasmid carrying the type IV system and comparison of this region in the three strains. The scale on the right indicates the G+C content values in this region. The black curve indicates variations in G+C content along this region in strain Lens. Dark grey bars, homologous regions in the three genomes; light grey arrow, genes predicted in this region. Arrows and numbers indicate the insertion position on the chromosome of each strain.

Citation: Cazalet C, Jarraud S, Ghavi-Helm Y, Allignet J, Kunst F, Etienne J, Glaser P, Buchrieser C. 2006. Genetic Diversity of , p 355-358. In Cianciotto N, Kwaik Y, Edelstein P, Fields B, Geary D, Harrison T, Joseph C, Ratcliff R, Stout J, Swanson M (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555815660.ch86
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References

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1. Aurell, H.,, J. Etienne,, F. Forey,, M. Reyrolle,, P. Girardo,, P. Farge,, B. Decludt,, F. Vandenesch, and, S. Jarraud. 2003. Legionella pneumophila serogroup 1 strain Paris: endemic distribution throughout France. J. Clin. Microbiol. 41:3320—3322.
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