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Chapter 91 : The Amoeba Contributes to Infection

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Abstract:

The haploid social amoeba is a well-defined model organism which is amenable to diverse biochemical, cell biological, and molecular genetic approaches. The similarities between the and mammalian cells extend to membrane trafficking, endocytic transit and sorting events. Therefore, opens the exciting possibility of investigating and manipulating both sides of the -host interaction. The intrinsic features of and a set of well-established molecular tools enabled the authors to prove that is a representative cellular model for infection. The research areas presented in this chapter include the analysis of the transcriptional host cell response to infection and the use of custom tailored mutant cells to identify determinants of susceptibility and resistance. In order to describe the transcriptional host cell response to infection, the authors employed DNA-microarrays which carried approximately half of the genes. Mammalian Nramp1 decorates endosomes, phagosomes, and postlysosomal vesicles of macrophages, and in it has been shown that Nramp1 depletes iron from the phagolysosome in an ATP-dependent process. Three approaches were used to analyze the complex cross-talk between and . First, microarrays were used to study the systemic host response to infection. Second, fluorescently tagged host cell factors were used to get new insights into the temporal and dynamic interactions between the pathogen and the host. Third, custom tailored mutants and specific inhibitors were used to functionally analyze infection relevant determinants of host susceptibility and resistance.

Citation: Steinert M, Wagner C, Fajardo M, Shevchuk O, Ünal C, Galka F, Heuner K, Eichinger L, Bozzaro S. 2006. The Amoeba Contributes to Infection, p 390-394. In Cianciotto N, Kwaik Y, Edelstein P, Fields B, Geary D, Harrison T, Joseph C, Ratcliff R, Stout J, Swanson M (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555815660.ch91

Key Concept Ranking

Dictyostelium discoideum
0.68648547
Legionella pneumophila
0.6298784
0.68648547
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Figures

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FIGURE 1

Road map of host cell factors involved in infection. The arrows in brackets indicate the up- and downregulation of the corresponding genes.

Citation: Steinert M, Wagner C, Fajardo M, Shevchuk O, Ünal C, Galka F, Heuner K, Eichinger L, Bozzaro S. 2006. The Amoeba Contributes to Infection, p 390-394. In Cianciotto N, Kwaik Y, Edelstein P, Fields B, Geary D, Harrison T, Joseph C, Ratcliff R, Stout J, Swanson M (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555815660.ch91
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References

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Tables

Generic image for table
TABLE 1

Number of differentially expressed genes during the time course of infection

Citation: Steinert M, Wagner C, Fajardo M, Shevchuk O, Ünal C, Galka F, Heuner K, Eichinger L, Bozzaro S. 2006. The Amoeba Contributes to Infection, p 390-394. In Cianciotto N, Kwaik Y, Edelstein P, Fields B, Geary D, Harrison T, Joseph C, Ratcliff R, Stout J, Swanson M (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555815660.ch91

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