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Chapter 92 : Strongly Increases the Number of in Model Tap Water Biofilms

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Strongly Increases the Number of in Model Tap Water Biofilms, Page 1 of 2

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Abstract:

Recently the authors demonstrated through several uptake experiments that some bacterial species do not act as competitors on uptake by , but are able to influence intracellular Legionella replication. Biofilm experiments were performed at 35°C in a rotating annular reactor. Experiments were repeated at least three times and always consisted of two parts: during the first part, without present (days 0 to 36), the authors investigated if were able to attach and eventually grow in the biofilm. During the second part, trophozoites were added to the system and the evolution of biofilm-associated was investigated. Addition of on day 36 resulted within 48 h in a significant decrease of biofilm-associated non- bacteria due to predation, while biofilm-associated increased with 1.5 log units due to intracellular replication in the present trophozoites. The association between Legionnaires’ disease and the presence of high numbers of human pathogenic bacteria in man-made water supplies has already been confirmed frequently. Therefore, the results stress the importance of further obtaining data concerning interactions between and potential amoeba host populations associated with biofilms.

Citation: Declerck P, Behets J, Lammertyn E, Ollevier F. 2006. Strongly Increases the Number of in Model Tap Water Biofilms, p 395-397. In Cianciotto N, Kwaik Y, Edelstein P, Fields B, Geary D, Harrison T, Joseph C, Ratcliff R, Stout J, Swanson M (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555815660.ch92

Key Concept Ranking

Legionella pneumophila
0.9303865
Acanthamoeba castellanii
0.675
0.9303865
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Figures

Image of FIGURE 1
FIGURE 1

Evolution of the base biofilm, biofilm-associated and n = 2; log x̄ ± 95% confidence intervals. The arrows indicate the inoculation of and in the system.

Citation: Declerck P, Behets J, Lammertyn E, Ollevier F. 2006. Strongly Increases the Number of in Model Tap Water Biofilms, p 395-397. In Cianciotto N, Kwaik Y, Edelstein P, Fields B, Geary D, Harrison T, Joseph C, Ratcliff R, Stout J, Swanson M (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555815660.ch92
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References

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1. Declerck, P.,, J. Behets,, Y. Delaedt,, A. Mar-gineanu,, E. Lammertyn, and, F. Ollevier. 2005. Impact of non-Legionella bacteria on the uptake and intracellular replication of Legionella pneumophila in Acanthamoeba castellanii and Naegleria lovaniensis. Microbial Ecol. 50:536549.
2. Declerck, P.,, J. Behets,, E. Lammertyn, and, F. Ollevier. 2006. Whole cell fluorescent in situ hybridization (FISH) of Legionella in various kinds of samples. In L. O’Connor (ed.). Diagnostic Bacteriology Protocols, 2nd ed. Humana Press, Totowa, NJ 345:175184.
3. Herpers, B. L.,, B. M. de Jongh,, K. van der Zwaluw, and, E. J. van Hannen. 2003. Real-time PCR assay targets the 23S-5S spacer for direct detection and differentiation of Legionella spp. and Legionella pneumophila. J. Clin. Microbiol. 41:48154816.
4. Meigh, R. E.,, T. Makin,, M. H. Scott, and, C. A. Hart. 1989. Legionella pneumophila serogroup 12 pneumonia in a renal transplant recipient: case report and environmental observations. J. Hosp. Infect. 13:315319.
5. Noble, J. A.,, D. G. Ahearn,, S. V. Avery, and, S. A. Crow. 2002. Phagocytosis affects biguanide sensitivity of Acanthamoeba spp. Antimicrob. Agents Chemother. 46:20692076.
6. Percival, S. L.,, J. T. Walker, and, P. R. Hunter (ed.). 2000. In Microbiological Aspects of Biofilms and Drinking Water. CRC Press, Boca Raton, FL, p. 240.
7. Rivière, D.,, F. M. Szczebara,, J. M. Berjeaud,, J. Frère, and, Y. Héchard. 2006. Development of a real-time PCR assay for quantification of Acan-thamoeba trophozoites and cysts. J. Microbiol. Methods 64:7883.

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