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Chapter 94 : Evaluation of Signaling between Legionella pneumophila Multiple Prokaryotes
The association of legionellae with microorganisms in the aquatic environment has been documented earlier. This relationship includes its cohabitation with protozoa, algae, and prokaryotes within biofilms. Potential candidates are the intercellular signals produced by bacterial species commonly isolated from biofilm communities. Acyl-homoserine lactones (AHSL) and furanosyl borate diesters (AI-2) are two prokaryotic signaling compounds that have been isolated from biofilms. The objective of this chapter is to evaluate the capacity of Legionella pneumophila to respond to interspecific signals. From evaluation of protein expression after exposure to various bacterial supernatants, multiple proteins were identified as products of L. pneumophila's response to heterospecific signals. Spots of interest were manually excised from two-dimensional electrophoresis gels, washed, and subjected to in situ digestion with trypsin, and the resultant peptides were desalted and concentrated with C18ZipTips (Millipore). AI-2 has been detected in numerous prokaryotes, both gram-positive and gram-negative, and is commonly referred to as a universal communication signal among microorganisms.