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Chapter 94 : Evaluation of Signaling between Multiple Prokaryotes

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Evaluation of Signaling between Multiple Prokaryotes, Page 1 of 2

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Abstract:

The association of legionellae with microorganisms in the aquatic environment has been documented earlier. This relationship includes its cohabitation with protozoa, algae, and prokaryotes within biofilms. Potential candidates are the intercellular signals produced by bacterial species commonly isolated from biofilm communities. Acyl-homoserine lactones (AHSL) and furanosyl borate diesters (AI-2) are two prokaryotic signaling compounds that have been isolated from biofilms. The objective of this chapter is to evaluate the capacity of to respond to interspecific signals. From evaluation of protein expression after exposure to various bacterial supernatants, multiple proteins were identified as products of response to heterospecific signals. Spots of interest were manually excised from two-dimensional electrophoresis gels, washed, and subjected to in situ digestion with trypsin, and the resultant peptides were desalted and concentrated with CZipTips (Millipore). AI-2 has been detected in numerous prokaryotes, both gram-positive and gram-negative, and is commonly referred to as a universal communication signal among microorganisms.

Citation: D. Zeigler-Ballerstein S, M. Barbaree J. 2006. Evaluation of Signaling between Multiple Prokaryotes, p 403-406. In Cianciotto N, Kwaik Y, Edelstein P, Fields B, Geary D, Harrison T, Joseph C, Ratcliff R, Stout J, Swanson M (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555815660.ch94

Key Concept Ranking

Legionella pneumophila
0.54406136
Klebsiella pneumoniae
0.5326401
0.54406136
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Figures

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FIGURE 1

Proteome of the control culture with a comparison of differentially expressed proteins.

Citation: D. Zeigler-Ballerstein S, M. Barbaree J. 2006. Evaluation of Signaling between Multiple Prokaryotes, p 403-406. In Cianciotto N, Kwaik Y, Edelstein P, Fields B, Geary D, Harrison T, Joseph C, Ratcliff R, Stout J, Swanson M (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555815660.ch94
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References

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1. Davies, D. G.,, M. R. Parsek,, J. P. Pearson,, B. H. Iglewski,, J. W. Costerton, and, E. P. Greenberg. 1998. The involvement of cell-to-cell signals in the development of a bacterial biofilm. Science 280:295298.
2. Duan, K,, C. Dammel,, J. Stein,, H. Rabin, and, M. G. Surette. 2003. Modulation of Pseudomonas aeruginosa gene expression by host mi-croflora through interspecies communication. Mol. Microbiol. 50:14771491.
3. Lebeau, I.,, E. Lammertyn,, E. De Buck,, L. Maes,, N. Geukens,, L. Van Mellaert,, L. Arck-ens,, J. Anne, and, S. Clerens. 2004. First pro-teomic analysis of Legionella pneumophila based on its developing genome sequence. Res. Microbiol. 256:119129.
4. Miller, M. B., and, B. L. Bassler. 2001. Quorum sensing in bacteria. Annu. Rev. Microbiol. 55: 165199.
5. Wadowsky, R. M., and, R. B. Yee. 1985. Effects on non-Legionellaceae bacteria on the multiplication of Legionella pneumophila in potable water. Appl. Environ. Micobiol. 49:12061210.
6. Westermeier, R., and, T. Naven. 2002. Proteomics in Practice: A Laboratory Manual. Wiley-VCH.

Tables

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TABLE 1

Partial list of proteins identified through MALDI and MASCOT analysis

Citation: D. Zeigler-Ballerstein S, M. Barbaree J. 2006. Evaluation of Signaling between Multiple Prokaryotes, p 403-406. In Cianciotto N, Kwaik Y, Edelstein P, Fields B, Geary D, Harrison T, Joseph C, Ratcliff R, Stout J, Swanson M (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555815660.ch94

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