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Chapter 98 : Diversity of spp. in Antarctic Lakes of the Keller Peninsula

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Diversity of spp. in Antarctic Lakes of the Keller Peninsula, Page 1 of 2

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Abstract:

The use of culture-independent molecular screening techniques has allowed the description of as a member of the microbial community structure of extreme environments such as polar regions. The authors used traditional culture and coculture methods to isolate spp. and independent-culture methods to verify and compare the diversity of the population in the microbial communities of two Antarctic lakes. Water samples (5 liters) were collected from North and South Lakes, near the Brazilian Scientific Station Comandante Ferraz, located in the Keller Peninsula, King George Island, Antarctica. Colonies were obtained only from the North Lake samples. Selected colonies were used for total genomic DNA extraction. Denaturing gradient gel electrophoresis (DGGE) analysis from the water samples showed 28 to 30 bands representing abundant and similar phylotypes between the bacterial structures of both lakes. The DGGE technique demonstrated that is a significant member of the community in both lakes. Such findings could be related to the presence of ferric ions determined in the studied areas of Keller Peninsula. The isolation of from these Antarctic lakes will allow future studies in cold-resistance mechanisms of mesophilic bacteria in polar environments.

Citation: R. S. Carvalho F, R. Nastasi F, C. Gamba R, S. Foronda A, H. Pellizari V. 2006. Diversity of spp. in Antarctic Lakes of the Keller Peninsula, p 417-419. In Cianciotto N, Kwaik Y, Edelstein P, Fields B, Geary D, Harrison T, Joseph C, Ratcliff R, Stout J, Swanson M (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555815660.ch98

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Denaturing Gradient Gel Electrophoresis
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Figures

Image of FIGURE 1
FIGURE 1

Silver stained DGGE-PCR gel (V3 variable region from 16S rRNA gene). (A through E) strains ATCC 33152, 33153, 33154, 33155, and 33156; (F and G) strains isolated from North Lake; (H and I) total bacterial communities from the North and South Lakes, respectively. White arrows indicate the comparison between bands of ATCC strains, isolated strains, and microbial communities analyzed.

Citation: R. S. Carvalho F, R. Nastasi F, C. Gamba R, S. Foronda A, H. Pellizari V. 2006. Diversity of spp. in Antarctic Lakes of the Keller Peninsula, p 417-419. In Cianciotto N, Kwaik Y, Edelstein P, Fields B, Geary D, Harrison T, Joseph C, Ratcliff R, Stout J, Swanson M (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555815660.ch98
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Image of FIGURE 2
FIGURE 2

Unrooted phyloge-netic tree of reference strains, culture obtained in this study, and clones from 16S rRNA gene from North Lake (LN) and South Lake (LS). The dendrogram was generated by using MEGA software package version 3.0. Bootstrap analyses were applied to the data ( = 10,000).

Citation: R. S. Carvalho F, R. Nastasi F, C. Gamba R, S. Foronda A, H. Pellizari V. 2006. Diversity of spp. in Antarctic Lakes of the Keller Peninsula, p 417-419. In Cianciotto N, Kwaik Y, Edelstein P, Fields B, Geary D, Harrison T, Joseph C, Ratcliff R, Stout J, Swanson M (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555815660.ch98
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References

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