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Chapter 2 : Isolation, Enumeration, and Preservation of the

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Isolation, Enumeration, and Preservation of the , Page 1 of 2

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Abstract:

One or a combination of the bacteriocidal agents is often present in most of the selective media commonly used for the selective isolation of vibrios. A scheme for the isolation of pathogenic vibrios often includes enrichment prior to plating on a selective agar, especially when vibrios are at low levels and competing with other bacteria that can outgrow them in general media. Several enrichment media have been used for the selection of one group or species of . Special effort has been devoted to enhance the presence of potential pathogenic species in contaminated food or in clinical samples. Thiosulfate citrate bile salt sucrose agar (TCBS) is an ideal medium for the selective isolation and purification of vibrios. Although this medium was originally designed for the isolation of and , most vibrios grow to healthy large colonies with many different colonial morphologies. Until now, TCBS was the best selective medium for isolation of vibrios, though some strains of , , , , , and may present slight growth on it. TCBS agar is also an excellent medium for the selective isolation of vibrios from environmental sources, but differences in media formulation between manufacturers might be reflected in the enumeration of vibrios. Enumeration molecular techniques include the use of polymerase chain reaction (PCR) analysis or DNA probes for colony hybridization. Often, vibrios recovered directly from cryopreservation require at least two passes to fully recuperate and longer incubation periods.

Citation: Gomez-Gil B, Roque A. 2006. Isolation, Enumeration, and Preservation of the , p 15-26. In Thompson F, Austin B, Swings J (ed), The Biology of Vibrios. ASM Press, Washington, DC. doi: 10.1128/9781555815714.ch2

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Polymyxin B
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Vibrio vulnificus
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References

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Tables

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TABLE 1

Media used for growth of medically important vibrios (adapted from Donovan and van Netten, 1995)

Citation: Gomez-Gil B, Roque A. 2006. Isolation, Enumeration, and Preservation of the , p 15-26. In Thompson F, Austin B, Swings J (ed), The Biology of Vibrios. ASM Press, Washington, DC. doi: 10.1128/9781555815714.ch2

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