Chapter 31 : Large-Scale Production of Microorganisms

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This chapter highlights the selection and design of equipment and facilities to achieve a safe work environment. There are few absolutes that can be applied across the board, since the decisions to be made are dependent on the risk assessment of the organism and the processes used. Fortunately, similar equipment and facility design criteria are used, so there are common biosafety principles that can be utilized which are discussed herein. Primary containment is provided by the equipment utilized and the use of appropriate biosafety practices, administrative practices, and personal protective equipment. In general, all of the standard and special practices and safety equipment identified in (BMBL), along with the recommendations in the NIH recombinant-DNA guidelines, are applicable to large-scale processes. Bioreactors used for growth of microorganisms and those used for cell culture can share many attributes. A biological safety cabinet (BSC) can be utilized for smaller equipment that does not generate much turbulence. In some instances, BSC manufacturers, or other specialty equipment fabricators, can make specialized containment devices for specific equipment. The facility design and construction provide the secondary containment that protects people outside of the immediate work area, both in other parts of the facility and in the community at large.

Citation: Cipriano M. 2006. Large-Scale Production of Microorganisms, p 561-577. In Fleming D, Hunt D (ed), Biological Safety. ASM Press, Washington, DC. doi: 10.1128/9781555815899.ch31

Key Concept Ranking

Saccharomyces cerevisiae
Risk Assessment
Escherichia coli
Infectious Dose
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1. Advisory Committee on Dangerous Pathogens. 1995. Categorization of Biological Agents According to Hazards and Categories of Containment, 4th ed. Her Majesty’s Stationery Office, London, United Kingdom.
2. Advisory Committee on Dangerous Pathogens. 1998. The Large Scale Contained Use of Biological Agents. Her Majesty’s Stationery Office, London, England.
3. Bailey, J. E., and, D. F. Ollis. 1977. Biochemical Engineering Fundamentals, p. 574–634. McGraw Hill, New York, N.Y.
4. Centers for Disease Control and Prevention and National Institutes of Health. 1999. Biosafety in Microbiological and Biomedical Laboratories, 4th ed. U.S. Government Printing Office, Washington, D.C.
5. Centers for Disease Control and Prevention and National Institutes of Health. 2000. Primary Containment for Biohazards: Selection, Installation and Use of Biological Safety Cabinets, 2nd ed. J. Y. Richmond and, R. W. McKinney, (ed.). U.S. Government Printing Office, Washington, D.C. Available online at http://www.cdc.gov/od/ohs/pdffiles/BSC-3.pdf.
6. Ghidoni, D. A. 1999. HVAC issues in secondary containment, p. 63–72. In J. Y. Richmond (ed.), Anthology of Biosafety. American Biological Safety Association, Mundelein, Ill.
7. Hambleton,, P., J. Melling, and, T. T. Salusbury (ed.). 1994. Biosafety in Industrial Biotechnology. Blackie Academic & Professional, Glasgow, Scotland.
8. Liberman,, D. F., R. Fink, and, F. Schaefer. 1986. Biosafety in biotechnology, p. 402–408. In A. L. Solomon and, N. A. Demain. (ed.). Industrial Microbiology and Biotechnology. American Society for Microbiology, Washington, D.C.
9. McGarrity,, G. J., and, C. L. Hoerner. 1995. Biological safety in the biotechnology industry, P. 119–129. In D. O. Bosenge,, J. H. Richardson,, J. J. Tulis, and, D. Vesley (ed.), Laboratory Safety: Principles and Practices, 2nd ed. ASM Press, Washington, D.C.,
10. National Institutes of Health. 2002. NIH Guidelines for Research Involving Recombinant DNA Molecules (NIH Guidelines), 59 FR 34496 (July 5, 1994), as amended. [Online; the current version can be accessed at http://www4.od.nih.gov/oba/rac/guidelines/guidelines.html.
11. National Research Council. 1989. Biosafety in the Laboratory. National Academy Press, Washington, D.C.
12. Odum, J. 1995. Fundamental guidelines for biotech multiuse facilities, Pharm. Eng. 15:820.
13. Office of Laboratory Security, Public Health Agency of Canada, Health and Welfare Canada. 2004. Laboratory Biosafety Guidelines, 3rd ed. Health and Welfare Canada, Ottawa, Canada. Available online at http://www.phacaspc.gc.ca/publicat/lbg-ldmbl-04/pdf/lbg_2004_e.pdf.
14. Organisation for Economic Co-operation and Develop ment. 1992. Safety Considerations for Biotechnology. OECD Publications, Paris, France.
15. Prime Minister. 1991. Guidelines for Recombinant DNA Experiments. Ministry of Health, Tokyo, Japan.
16. World Health Organization. 2003. Guidelines for the Safe Production and Quality Control of IPV Manufactured from Wild Polio Virus. World Health Organization, Geneva, Switzerland.
17. World Health Organization. 2004. Laboratory Biosafety Manual. 3rd ed. World Health Organization, Geneva, Switzerland.

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