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Chapter 33 : Quantification of Recent Thymic Emigrants: T-Cell-Receptor Excision Circles

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Abstract:

In chickens, blood lymphocytes may be identified as recent thymic emigrants (RTEs) by the use of antibodies that react with the cell surface marker chT1. PCR quantification of T-cell receptor (TCR) rearrangement excision circles (TRECs) derived from TCR β chain locus rearrangement has been described and has been used to provide additional evidence that the differentiation of T cells with a diverse TCR repertoire continues until late adulthood. The quantitation of TRECs may be performed with unsorted cells or with subpopulations of cells purified by fluorescent cell sorting, magnetic bead separation, panning, or other techniques. In view of its high yield and relative simplicity, magnetic cell sorting with CD4 and CD8 microbeads and positive selection columns is often used. High TREC concentrations have been associated with changes in T-cell populations after the transplantation of thymic tissue in patients with DiGeorge syndrome and, in some cases, after highly active antiretroviral therapy (HAART) for human immunodeficiency virus (HIV) infection. Moreover, recent data suggest that TREC values may prove to be a useful predictor of disease progression for HIV infection.

Citation: Krogstad P. 2006. Quantification of Recent Thymic Emigrants: T-Cell-Receptor Excision Circles, p 291-295. In Detrick B, Hamilton R, Folds J (ed), Manual of Molecular and Clinical Laboratory Immunology, 7th Edition. ASM Press, Washington, DC. doi: 10.1128/9781555815905.ch33

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Figures

Image of FIGURE 1
FIGURE 1

Production of α TREC. The TCRD locus is shown embedded within TCRA. Recombination releases an 89-kbp signal-joint TREC containing a distinctive sequence that may be detected by oligonucleotide primers spaced approximately 180 bp from either side of the joint. A second recombination event brings the Vα and Jα sequences into contiguity, releasing a second TREC containing the coding joint (not shown). (Modified from reference with permission of the publishers.)

Citation: Krogstad P. 2006. Quantification of Recent Thymic Emigrants: T-Cell-Receptor Excision Circles, p 291-295. In Detrick B, Hamilton R, Folds J (ed), Manual of Molecular and Clinical Laboratory Immunology, 7th Edition. ASM Press, Washington, DC. doi: 10.1128/9781555815905.ch33
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References

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1. Bell, E. B., and , S. M. Sparshott. 1990. Interconversion of CD45R subsets of CD4 T cells in vivo. Nature 348:163166.
2. Chavan, S.,, B. Bennuri,, M. Kharbanda,, A. Chandrasekaran,, S. Bakshi, and , S. Pahwa. 2001. Evaluation of T cell receptor gene rearrangement excision circles after antiretroviral therapy in children infected with human immunodeficiency virus. J. Infect. Dis. 183:14451454.
3. Deeks, S. G. 2003. Treatment of antiretroviral-drugresistant HIV-1 infection. Lancet 362:20022011.
4. De Rosa, S. C.,, L. A. Herzenberg, and , M. Roederer. 2001. 11-color, 13-parameter flow cytometry: identification of human naive T cells by phenotype, function, and T-cell receptor diversity. Nat. Med. 7:245248.
5. Douek, D. C.,, R. D. McFarland,, P. H. Keiser,, E. A. Gage,, J. M. Massey,, B. F. Haynes,, M. A. Polis,, A. T. Haase,, M. B. Feinberg,, J. L. Sullivan,, B. D. Jamieson,, J. A. Zack,, L. J. Picker, and , R. A. Koup. 1998. Changes in thymic function with age and during the treatment of HIV infection. Nature 396:690695.
6. Flores, K. G.,, J. Li,, G. D. Sempowski,, B. F. Haynes, and , L. P. Hale. 1999. Analysis of the human thymic perivascular space during aging. J. Clin. Invest. 104:10311039.
7. Halnon, N.,, B. Jamieson,, M. Plunkett,, C. Kitchen,, T. Pham, and , P. Krogstad. 2004. Thymic function and impaired maintenance of peripheral T cell populations in children with congenital heart disease and surgical thymectomy. Pediatr. Res. 57:4248.
8. Hargreaves, M., and , E. B. Bell. 1997. Identical expression of CD45R isoforms by CD45RC+ “revertant” memory and CD45RC+ naive CD4 T cells. Immunology 91:323330.
9. Hatzakis, A.,, G. Touloumi,, R. Karanicolas,, A. Karafoulidou,, T. Mandalaki,, C. Anastassopoulou,, L. Zhang,, J. J. Goedert,, D. D. Ho, and , L. G. Kostrikis. 2000. Effect of recent thymic emigrants on progression of HIV-1 disease. Lancet 355:599604.
10. Haynes, B. F.,, M. L. Markert,, G. D. Sempowski,, D. D. Patel, and , L. P. Hale. 2000. The role of the thymus in immune reconstitution in aging, bone marrow transplantation, and HIV-1 infection. Annu. Rev. Immunol. 18:529560.
11. Hazenberg, M. D.,, M. C. Verschuren,, D. Hamann,, F. Miedema, and , J. J. van Dongen. 2001. T cell receptor excision circles as markers for recent thymic emigrants: basic aspects, technical approach, and guidelines for interpretation. J. Mol. Med. 79:631640.
12. Hellerstein, M. K. 1999. Measurement of T-cell kinetics: recent methodologic advances. Immunol. Today 20:438441.
13. Jamieson, B. D.,, D. C. Douek,, S. Killian,, L. E. Hultin,, D. D. Scripture-Adams,, J. V. Giorgi,, D. Marelli,, R. A. Koup, and , J. A. Zack. 1999. Generation of functional thymocytes in the human adult. Immunity 10:569575.
14. Kong, F.,, C. H. Chen, and , M. D. Cooper. 1998. Thymic function can be accurately monitored by the level of recent T cell emigrants in the circulation. Immunity 8:97104.
15. Ledergerber, B.,, M. Egger,, M. Opravil,, A. Telenti,, B. Hirschel,, M. Battegay,, P. Vernazza,, P. Sudre,, M. Flepp,, H. Furrer,, P. Francioli, and , R. Weber. 1999. Clinical progression and virological failure on highly active antiretroviral therapy in HIV-1 patients: a prospective cohort study. Swiss HIV Cohort Study. Lancet 353:863868.
16. Mackall, C. L.,, T. A. Fleisher,, M. R. Brown,, M. P. Andrich,, C. C. Chen,, I. M. Feuerstein,, M. E. Horowitz,, I. T. Magrath,, A. T. Shad,, S. M. Steinberg, et al. 1995. Age, thymopoiesis, and CD4+ T-lymphocyte regeneration after intensive chemotherapy. New Engl. J. Med. 332(3): 143149.
17. Markert, M. L.,, A. Boeck,, L. P. Hale,, A. L. Kloster,, T. M. McLaughlin,, M. N. Batchvarova,, D. C. Douek,, R. A. Koup,, D. D. Kostyu,, F. E. Ward,, H. E. Rice,, S. M. Mahaffey,, S. E. Schiff,, R. H. Buckley, and , B. F. Haynes. 1999. Transplantation of thymus tissue in complete DiGeorge syndrome. New Engl. J. Med. 341 (16):11801189.
18. Pham, T.,, M. Belzer,, J. A. Church,, C. Kitchen,, C. M. Wilson,, S. D. Douglas,, Y. Geng,, M. Silva,, R. M. Mitchell, and , P. Krogstad. 2003. Assessment of thymic activity in human immunodeficiency virus-negative and -positive adolescents by real-time PCR quantitation of T-cell receptor rearrangement excision circles. Clin. Diagn. Lab. Immunol. 10:323328.
19. Poulin, J. F.,, M. N. Viswanathan,, J. M. Harris,, K. V. Komanduri,, E. Wieder,, N. Ringuette,, M. Jenkins,, J. M. McCune, and , R. P. Sekaly. 1999. Direct evidence for thymic function in adult humans. J. Exp. Med. 190:479486.
20. Sempowski, G.,, J. Thomasch,, M. Gooding,, L. Hale,, L. Edwards,, E. Ciafaloni,, D. Sanders,, J. Massey,, D. Douek,, R. Koup, and , B. Haynes. 2001. Effect of thymectomy on human peripheral blood T cell pools in myasthenia gravis. J. Immunol. 166:28082817.
21. Tough, D. F., and , J. Sprent. 1995. Life span of naive and memory T cells. Stem Cells 13(3):242249.
22. Verschuren, M. C.,, I. L. Wolvers-Tettero,, T. M. Breit,, J. Noordzij,, E. R. van Wering, and , J. J. van Dongen. 1997. Preferential rearrangements of the T cell receptor-delta-deleting elements in human T cells. J. Immunol. 158:12081216.
23. Zack, J. A.,, S. J. Arrigo,, S. R. Weitsman,, A. S. Go,, A. Haislip, and , I. S. Chen. 1990. HIV-1 entry into quiescent primary lymphocytes: molecular analysis reveals a labile, latent viral structure. Cell 61:213222.
24. Zhang, L.,, S. R. Lewin,, M. Markowitz,, H. H. Lin,, E. Skulsky,, R. Karanicolas,, Y. He,, X. Jin,, S. Tuttleton,, M. Vesanen,, H. Spiegel,, R. Kost,, J. van Lunzen,, H. J. Stellbrink,, S. Wolinsky,, W. Borkowsky,, P. Palumbo,, L. G. Kostrikis, and , D. D. Ho. 1999. Measuring recent thymic emigrants in blood of normal and HIV-1-infected individuals before and after effective therapy. J. Exp. Med. 190(5):725732.

Tables

Generic image for table
TABLE 1

PCR primer and probe sequences

Citation: Krogstad P. 2006. Quantification of Recent Thymic Emigrants: T-Cell-Receptor Excision Circles, p 291-295. In Detrick B, Hamilton R, Folds J (ed), Manual of Molecular and Clinical Laboratory Immunology, 7th Edition. ASM Press, Washington, DC. doi: 10.1128/9781555815905.ch33

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