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Chapter 45 : Immunohistochemistry: Principles and Advances

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Abstract:

This chapter presents current, practical methods that are applicable for performance of quality immunostains in the modern automated immunohistochemistry (IHC) laboratory. The cross-links are believed to be responsible for masking antibody-binding epitopes and to be a major cause of lack of sensitivity in paraffin section IHC. In spite of its inherent detrimental effects on antigenicity and IHC, a proper and consistent protocol for fixation with neutrally buffered formalin is imperative for high-quality IHC. Paraffin tissue sections for IHC are usually cut at a thickness of 4 to 6 μm. In order to retain sections on the glass microscope slide throughout the staining process, it is necessary to use slides that have been specially treated to increase their adhesive properties. Most antibodies used in a clinical IHC laboratory are commercially available and have well-characterized specificities. For analyte-specific reagents, it is assumed that good manufacturing practices have been followed and that the antibody will likely perform as advertised. Antigen retrieval enables antibodies that might previously have performed only in frozen tissues to be reactive in fixed tissues and has greatly expanded the number of antibodies that can now be applied to paraffin-embedded tissue.

Citation: Roche P, Hsi E, Firfer B. 2006. Immunohistochemistry: Principles and Advances, p 396-402. In Detrick B, Hamilton R, Folds J (ed), Manual of Molecular and Clinical Laboratory Immunology, 7th Edition. ASM Press, Washington, DC. doi: 10.1128/9781555815905.ch45

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References

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1. Anonymous. 1998. Medical devices: classification/reclassi-fication of immunohistochemistry reagents and kits—FDA. Final rule. Fed. Regist. 63:3013230142.
2. Battifora, H. 1991. Assessment of antigen damage in immunohistochemistry. The vimentin internal control. Am. J. Clin. Pathol. 96:669671.
3. Battifora, H., and , M. Kopinski. 1986. The influence of protease digestion and duration of fixation on the immunostaining of keratins. A comparison of formalin and ethanol fixation. J. Histochem. Cytochem. 34:10951100.
4. Bostwick, D. G.,, N. al Annouf, and , C. Choi. 1994. Establishment of the formalin-free surgical pathology laboratory. Utility of an alcohol-based fixative. Arch. Pathol. Lab. Med. 118:298302.
5. Bussolati, G.,, P. Gugliotta,, M. Volante,, M. Pace, and , M. Papotti. 1997. Retrieved endogenous biotin: a novel marker and a potential pitfall in diagnostic immunohistochemistry. Histopathology 31:400407.
6. Cattoretti, G.,, S. Pileri,, C. Parravicini,, M. H. Becker,, S. Poggi,, C. Bifulco,, G. Key,, L. D’Amato,, E. Sabattini,, E. Feudale, et al. 1993. Antigen unmasking on formalinfixed, paraffin-embedded tissue sections. J. Pathol. 171:8398.
7. Chan, J. K. 2000. Advances in immunohistochemistry: impact on surgical pathology practice. Semin. Diagn. Pathol. 17:170177.
8. Cuevas, E. C.,, A. C. Bateman,, B. S. Wilkins,, P. A. Johnson,, J. H. Williams,, A. H. Lee,, D. B. Jones, and , D. H. Wright. 1994. Microwave antigen retrieval in immunocytochemistry: a study of 80 antibodies. J. Clin. Pathol. 47:448452.
9. Falini, B.,, S. Pileri,, P. L. Zinzani,, A. Carbone,, V. Zagonel,, C. Wolf-Peeters,, G. Verhoef,, F. Menestrina,, G. Todeschini,, M. Paulli,, M. Lazzarino,, R. Giardini,, A. Aiello,, H. D. Foss,, I. Araujo,, M. Fizzotti,, P. G. Pelicci,, L. Flenghi,, M. F. Martelli, and , A. Santucci. 1999. ALK+ lymphoma: clinico-pathological findings and outcome. Blood 93:26972706.
10. Fox, C. H.,, F. B. Johnson,, J. Whiting, and , P. P. Roller. 1985. Formaldehyde fixation. J. Histochem. Cytochem. 33:845853.
11. Gown, A. M. 2004. Unmasking the mysteries of antigen or epitope retrieval and formalin fixation. Am. J. Clin. Pathol. 121:172174.
12. Horne, G. M.,, J. J. Anderson,, D. G. Tiniakos,, G. G. McIntosh,, M. D. Thomas,, B. Angus,, J. A. Henry,, T. W. Lennard, and , C. H. Horne. 1996. p53 protein as a prognostic indicator in breast carcinoma: a comparison of four antibodies for immunohistochemistry Br. J. Cancer 73:2935.
13. Iezzoni, J. C.,, S. E. Mills,, T. J. Pelkey, and , M. H. Stoler. 1999. Inhibin is not an immunohistochemical marker for hepatocellular carcinoma. An example of the potential pitfall in diagnostic immunohistochemistry caused by endogenous biotin. Am. J. Clin. Pathol. 111:229234.
14. Jacobs, T. W.,, J. E. Prioleau,, I. E. Stillman, and , S. J. Schnitt. 1996. Loss of tumor marker-immunostaining intensity on stored paraffin slides of breast cancer. J. Natl. Cancer Inst. 88:10541059.
15. King, G.,, S. Payne,, F. Walker, and , G. I. Murray. 1997. A highly sensitive detection method for immunohistochemistry using biotinylated tyramine. J. Pathol. 183:237241.
16. Li, C. Y.,, S. C. Ziesmer, and , O. Lazcano-Villareal. 1987. Use of azide and hydrogen peroxide as an inhibitor for endogenous peroxidase in the immunoperoxidase method. J. Histochem. Cytochem. 35:14571460.
17. McCluggage, W. G.,, P. Maxwell,, A. Patterson, and , J. M. Sloan. 1997. Immunohistochemical staining of hepatocellular carcinoma with monoclonal antibody against inhibin. Histopathology 30:518522.
18. McNicol, A. M., and , J. A. Richmond. 1997. Optimizing immunohistochemistry: antigen retrieval and signal amplification. J. Histochem. Cytochem. 45:327343.
19. Montero, C.,, E. Chi-Ahumada,, S. Chavez-Porras, and , M. Gutierrez. 2000. Application of the critical molar concentration concept to heat-mediated antigen retrieval in immunohistochemistry. Histochem. J. 32:111114.
20. Morgan, J. M.,, H. Navabi,, K. W. Schmid, and , B. Jasani. 1994. Possible role of tissue-bound calcium ions in citrate-mediated high-temperature antigen retrieval. J. Pathol. 174:301307.
21. Pileri, S. A.,, G. Roncador,, C. Ceccarelli,, M. Piccioli,, A. Briskomatis,, E. Sabattini,, S. Ascani,, D. Santini,, P. P. Piccaluga,, O. Leone,, S. Damiani,, C. Ercolessi,, F. Sandri,, F. Pieri,, L. Leoncini, and , B. Falini. 1997. Antigen retrieval techniques in immunohistochemistry: comparison of different methods. J. Pathol. 183:116123.
22. Ponder, B. A., and , M. M. Wilkinson. 1981. Inhibition of endogenous tissue alkaline phosphatase with the use of alkaline phosphatase conjugates in immunohistochemistry. J. Histochem. Cytochem. 29:981984.
23. Rhodes, A.,, B. Jasani,, A. J. Balaton, and , K. D. Miller. 2000. Immunohistochemical demonstration of oestrogen and progesterone receptors: correlation of standards achieved on in house tumours with that achieved on external quality assessment material in over 150 laboratories from 26 countries. J. Clin. Pathol. 53:292301.
24. Rhodes, A.,, B. Jasani,, D. M. Barnes,, L. G. Bobrow, and , K. D. Miller. 2000. Reliability of immunohistochemical demonstration of oestrogen receptors in routine practice: interlaboratory variance in the sensitivity of detection and evaluation of scoring systems. J. Clin. Pathol. 53:125130.
25. Roche, P. C. 1998. Antigen stability in stored paraffin sections. CAP Today 12:5961.
26. Sabattini, E.,, K. Bisgaard,, S. Ascani,, S. Poggi,, M. Piccioli,, C. Ceccarelli,, F. Pieri,, G. Fraternali-Orcioni, and , S. A. Pileri. 1998. The EnVision++ system: a new immunohistochemical method for diagnostics and research. Critical comparison with the APAAP, ChemMate, CSA, LABC, and SABC techniques. J. Clin. Pathol. 51:506511.
27. Shi, S. R.,, R. J. Cote, and , C. R. Taylor. 1996. Antigen retrieval immunohistochemistry: past, present, and future. Biotech. Histochem. 71:190196.
28. Shi, S. R.,, R. J. Cote,, C. Yang,, C. Chen,, H. J. Xu,, W. F. Benedict, and , C. R. Taylor. 1997. Development of an optimal protocol for antigen retrieval: a “test battery” approach exemplified with reference to the staining of retinoblastoma protein (pRB) in formalin-fixed paraffin sections. Histopathology 31:400407.
29. Shi, S. R.,, R. J. Cote,, L. Young,, S. A. Imam, and , C. R. Taylor. 1999. Use of pH 9.5 Tris-HCl buffer containing 5% urea for antigen retrieval immunohistochemistry. Am. J. Clin. Pathol. 111:445448.
30. Shi, S. R.,, M. E. Key, and , K. L. Kalra. 1991. Antigen retrieval in formalin-fixed, paraffin-embedded tissues: an enhancement method for immunohistochemical staining based on microwave oven heating of tissue sections. J. Histochem. Cytochem. 39:741748.
31. Swanson, P. E. 1999. Labels, disclaimers, and rules (oh, my!). Analyte-specific reagents and practice of immunohistochemistry. Am. J. Clin. Pathol. 111:445448.
32. Taylor, C. R. 1999. FDA issues final rule for classification and reclassification of immunochemistry reagents and kits. Am. J. Clin. Pathol. 111:443444.
33. Taylor, C. R. 1998. Report from the Biological Stain Commission: FDA issues final rule for classification/reclassification of immunochemistry (IHC) reagents and kits. Biotech. Histochem. 73:175177.
34. Taylor, C. R., and , S. R. Shi. 1997. Immunohistochemistry: principles and practice, p. 369379. In N. R. Rose,, E. Conway de Macario,, J. D. Folds,, H. C. Lane, and , R. M. Nakamura (ed.), Manual of Clinical Laboratory Immunology, 5th ed. ASM Press, Washington, D.C.
35. Taylor, C. R.,, S. R. Shi,, C. Chen,, L. Young,, C. Yang, and , R. J. Cote. 1996. Comparative study of antigen retrieval heating methods: microwave, microwave and pressure cooker, autoclave, and steamer. Histochem. Cell Biol. 105:253260.
36. Van der Loos, C. M. 1999. Immunoenzyme Multiple Staining Methods. Bios Scientific Publishers Limited, Oxford, United Kingdom.
37. Van der Loos, C. M.,, J. van den Oord,, P. K. Das, and , H. J. Houthoff. 1988. Use of commercially available monoclonal antibodies for immunoenzyme double staining. Histochem. J. 20:409413.
38. Van der Loos, C. M.,, A. E. Becker, and , J. J. van den Oord. 1993. Practical suggestions for successful immunoenzyme double staining experiments. Histochem. J. 25:113.
39. Van der Loos, C. M.,, M. M. Marijianowski, and , A. E. Becker. 1994. Quantification in immunohistochemistry: the measurement of the ratios of collagen types I and II. Histochem. J. 26:347354.
40. von Wasielewski, R.,, M. Werner,, M. Nolte,, L. Wilkens, and , A. Georgii. 1994. Effects of antigen retrieval by microwave heating in formalin-fixed tissue sections on a broad panel of antibodies. Histochemistry 102:165172.
41. Werner, M.,, R. Von Wasielewski, and , P. Komminoth. 1996. Antigen retrieval, signal amplification and intensification in immunohistochemistry. Histochem. Cell Biol. 105:253260.
42. Wood, G. S., and , R. Warnke. 1981. Suppression of endogenous avidin-binding activity in tissues and its relevance to biotin-avidin detection systems. J. Histochem. Cytochem. 29:11961204.

Tables

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TABLE 1

Protocol for preparation of 10% paraformaldehyde stock solution

Citation: Roche P, Hsi E, Firfer B. 2006. Immunohistochemistry: Principles and Advances, p 396-402. In Detrick B, Hamilton R, Folds J (ed), Manual of Molecular and Clinical Laboratory Immunology, 7th Edition. ASM Press, Washington, DC. doi: 10.1128/9781555815905.ch45
Generic image for table
TABLE 2

Protocol for steam heat-induced antigen retrieval

Citation: Roche P, Hsi E, Firfer B. 2006. Immunohistochemistry: Principles and Advances, p 396-402. In Detrick B, Hamilton R, Folds J (ed), Manual of Molecular and Clinical Laboratory Immunology, 7th Edition. ASM Press, Washington, DC. doi: 10.1128/9781555815905.ch45

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