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Chapter 53 : Immunologic Methods for Diagnosis of Infections Caused by Diarrheagenic and

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Immunologic Methods for Diagnosis of Infections Caused by Diarrheagenic and , Page 1 of 2

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Abstract:

The families and encompass a heterogeneous group of gram-negative organisms. The organisms chosen for discussion in this chapter are recognized as major public health concerns because of their significant contribution to morbidity and mortality in both children and adults in less developed and industrialized countries. Cytotoxicity is usually examined under the microscope or can be quantified by colorimetric methods. Positive samples should be retested in the presence of Shiga toxins (Stx)-specific antibodies to confirm the specificity of the cytotoxic effect. The optimal concentration of antigen should be determined by standard enzyme-linked immunosorbent assay (ELISA) checkerboard titration, coating plates with different amounts of antigen and testing them with known positive and negative control sera during the standardization of the assay. Diagnosis of acute enterotoxigenic (ETEC) infections requires the detection of labile toxin (LT), ST, or toxin genes in isolated strains. Typical enteropathogenic (EPEC) strains carry both a chromosomally encoded (the locus of enterocyte effacement (LEE), which mediates a complex signal transduction phenotype on the target epithelial cell, and the EPEC adherence factor plasmid (EAF), which encodes the principal EPEC adhesin.

Citation: Pasetti M, Nataro J, Levine M. 2006. Immunologic Methods for Diagnosis of Infections Caused by Diarrheagenic and , p 448-461. In Detrick B, Hamilton R, Folds J (ed), Manual of Molecular and Clinical Laboratory Immunology, 7th Edition. ASM Press, Washington, DC. doi: 10.1128/9781555815905.ch53

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Clinical and Public Health
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Sandwich Enzyme-Linked Immunosorbent Assay
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References

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1. Ahren, C.,, C. Wenneras,, J. Holmgren, and , A. M. Svennerholm. 1993. Intestinal antibody response after oral immunization with a prototype cholera B subunit-colonization factor antigen enterotoxigenic Escherichia coli vaccine. Vaccine 11:929934.
2. Attridge, S. R.,, C. Johansson,, D. D. Trach,, F. Qadri, and , A. M. Svennerholm. 2002. Sensitive microplate assay for detection of bactericidal antibodies to Vibrio cholerae O139. Clin. Diagn. Lab. Immunol. 9:383387.
3. Attridge, S. R.,, F. Qadri,, M. J. Albert, and , P. A. Manning. 2000. Susceptibility of Vibrio cholerae O139 to antibody-dependent, complement-mediated bacteriolysis. Clin. Diagn. Lab. Immunol. 7:444450.
4. Bettelheim, K. A., and , L. Beutin. 2003. Rapid laboratory identification and characterization of verocytotoxigenic (Shiga toxin producing) Escherichia coli (VTEC/STEC). J. Appl. Microbiol. 95:205217.
5. Bhuiyan, N. A.,, F. Qadri,, A. S. Faruque,, M. A. Malek,, M. A. Salam,, F. Nato,, J. M. Fournier,, S. Chanteau,, D. A. Sack, and , N. G. Balakrish. 2003. Use of dipsticks for rapid diagnosis of cholera caused by Vibrio cholerae O1 and O139 from rectal swabs. J. Clin. Microbiol. 41:39393941.
6. Bitzan, M., and , H. Karch. 1992. Indirect hemagglutination assay for diagnosis of Escherichia coli O157 infection in patients with hemolytic-uremic syndrome. J. Clin. Microbiol. 30:11741178.
7. Carillo, L.,, R. H. Gilman,, R. E. Mantle,, N. Nunez,, J. Watanabe,, J. Moron,, V. Quispe, and A. Ramirez-Ramos and the Loyaza Cholera Working Group in Peru. 1994. Rapid detection of Vibrio cholerae O1 in stools of Peruvian cholera patients by using monoclonal immunodiagnostic kits. J. Clin. Microbiol. 32:856857.
8. Chaicumpa, W.,, P. Srimanote,, Y. Sakolvaree,, T. Kalampaheti,, M. Chongsa-Nguan,, P. Tapchaisri,, B. Eampokalap,, P. Moolasart,, G. B. Nair, and , P. Echeverria. 1998. Rapid diagnosis of cholera caused by Vibrio cholerae O139. J. Clin. Microbiol. 36:35953600.
9. Chart, H., and , C. Jenkins. 1999. The serodiagnosis of infections caused by Verocytotoxinproducing Escherichia coli. J. Appl. Microbiol. 86:731740.
10. Choo, R. E., and , M. A. Huestis. 2004. Oral fluid as a diagnostic tool. Clin. Chem. Lab. Med. 42:12731287.
11. Cohen, D.,, M. S. Green,, C. Block,, R. Slepon, and , I. Ofek. 1991. Prospective study of the association between serum antibodies to lipopolysaccharide O antigen and the attack rate of shigellosis. J. Clin. Microbiol. 29:386389.
12. Colwell, R. R.,, J. A. Hasan,, A. Huq,, L. Loomis,, R. J. Siebeling,, M. Torres,, S. Galvez,, S. Islam,, M. T. Tamplin, and , D. Bernstein. 1992. Development and evaluation of a rapid, simple, sensitive, monoclonal antibody-based coagglutination test for direct detection of Vibrio cholerae O1. FEMS Microbiol. Lett. 76:215219.
13. Crowther, J. R. 2001. The Elisa Guidebook. Humana Press Inc., Totowa, N.J.
14. Dutta, S.,, A. Chatterjee,, P. Dutta,, K. Rajendran,, S. Roy,, K. C. Pramanik, and , S. K. Bhattacharya. 2001. Sensitivity and performance characteristics of a direct PCR with stool samples in comparison to conventional techniques for diagnosis of Shigella and enteroinvasive Escherichia coli infection in children with acute diarrhoea in Calcutta, India. J. Med. Microbiol. 50:667674.
15. Faruque, S. M.,, N. Chowdhury,, M. Kamruzzaman,, Q. S. Ahmad,, A. S. Faruque,, M. A. Salam,, T. Ramamurthy,, G. B. Nair,, A. Weintraub, and , D. A. Sack. 2003. Reemergence of epidemic Vibrio cholerae O139, Bangladesh. Emerg. Infect. Dis. 9:11161122.
16. Gavin, P. J.,, L. R. Peterson,, A. C. Pasquariello,, J. Blackburn,, M. G. Hamming,, K. J. Kuo, and , R. B. Thomson, Jr. 2004. Evaluation of performance and potential clinical impact of ProSpecT Shiga toxin Escherichia coli microplate assay for detection of Shiga toxin-producing E. coli in stool samples. J. Clin. Microbiol. 42:16521656.
17. Hashimoto, Y.,, Y. Itho,, Y Fujinaga,, A. Q. Khan,, F. Sultana,, M. Miyake,, K. Hirose,, H. Yamamoto, and , T. Ezaki. 1995. Development of nested PCR based on the viaB sequence to detect Salmonella typhi. J. Clin. Microbiol. 33:775777.
18. Herath, H. M. 2003. Early diagnosis of typhoid fever by the detection of salivary IgA. J. Clin. Pathol. 56:694698.
19. Hirose, K.,, K. Itoh,, H. Nakajima,, T. Kurazono,, M. Yamaguchi,, K. Moriya,, T. Ezaki,, Y. Kawamura,, K. Tamura, and , H. Watanabe. 2002. Selective amplification of tyv (rfbE), prt (rfbS), viaB, and fliC genes by multiplex PCR for identification of Salmonella enterica serovars Typhi and Paratyphi A. J. Clin. Microbiol. 40:633636.
20. Houng, H. S.,, O. Sethabutr, and , P. Echeverria. 1997. A simple polymerase chain reaction technique to detect and differentiate Shigella and enteroinvasive Escherichia coli in human feces. Diagn. Microbiol. Infect. Dis. 28:1925.
21. House, D.,, J. Wain,, V. A. Ho,, T. S. Diep,, N. T. Chinh,, P. V. Bay,, H. Vinh,, M. Duc,, C. M. Parry,, G. Dougan,, N. J. White,, T. T. Hien, and , J. J. Farrar. 2001. Serology of typhoid fever in an area of endemicity and its relevance to diagnosis. J. Clin. Microbiol. 39:10021007.
22. Iyer, L.,, J. Vadivelu, and , S. D. Puthucheary. 2000. Detection of virulence associated genes, haemolysin and protease amongst Vibrio cholerae isolated in Malaysia. Epidemiol. Infect. 125:2734.
23. Kaper, J. B.,, J. P. Nataro, and , H. L. Mobley. 2004. Pathogenic Escherichia coli. Nat. Rev. Microbiol. 2:123140.
24. Karch, H.,, M. Bielaszewska,, M. Bitzan, and , H. Schmidt. 1999. Epidemiology and diagnosis of Shiga toxin-producing Escherichia coli infections. Diagn. Microbiol. Infect. Dis. 34:229243.
25. Kotloff, K. L.,, M. F. Pasetti,, E. M. Barry,, J. P. Nataro,, S. S. Wasserman,, M. B. Sztein,, W. D. Picking, and , M. M. Levine. 2004. Deletion in the Shigella enterotoxin genes further attenuates Shigella flexneri 2a bearing guanine auxotrophy in a phase 1 trial of CVD 1204 and CVD 1208. J. Infect. Dis. 190:17451754.
26. Levine, M. M.,, J. Galen,, C. O. Tacket,, E. M. Barry,, M. F. Pasetti, and , M. B. Sztein. 2004. Attenuated strains of Salmonella enterica serovar Typhi as live oral vaccines against typhoid fever, p. 479486. In M. M. Levine,, J. B. Kaper,, R. Rappuoli,, M. A. Liu, and , M. F. Good (ed.), New Generation Vaccines. Marcel Dekker, Inc., New York, N.Y.
27. Levine, M. M.,, O. Grados,, R. H. Gilman,, W. E. Woodward,, R. Solis-Plaza, and , W. Waldman. 1978. Diagnostic value of the Widal test in areas endemic for typhoid fever. Am. J. Trop. Med. Hyg. 27:795800.
28. Levine, M. M., and , O. S. Levine. 1994. Changes in human ecology and behavior in relation to the emergence of diarrheal diseases, including cholera. Proc. Natl. Acad. Sci. USA 91:23902394.
29. Levine, M. M.,, J. McEwen,, G. Losonsky,, M. Reymann,, I. Harari,, J. E. Brown,, D. N. Taylor,, A. Donohue-Rolfe,, D. Cohen, and , M. Bennish. 1992. Antibodies to shiga holotoxin and to two synthetic peptides of the B subunit in sera of patients with Shigella dysenteriae 1 dysentery. J. Clin. Microbiol. 30:16361641.
30. Levine, M. M., and , N. F. Pierce. 1992. Immunity and vaccine development, p. 285327. In D. Barua and , W. B. Greenough III (ed.), Cholera. Plenum Medical Book Company, New York, N.Y.
31. Levine, M. M.,, C. R. Young,, R. E. Black,, Y. Takeda, and , R. A. Finkelstein. 1985. Enzyme-linked immunosorbent assay to measure antibodies to purified heat-labile entero-toxins from human and porcine strains of Escherichia coli and to cholera toxin: application in serodiagnosis and sero-epidemiology J. Clin. Microbiol. 21:174179.
32. Li, J. W.,, X. Q. Shi,, F. H. Chao,, X. W. Wang,, J. L. Zheng. and , N. Song. 2004. A study on detecting and identifying enteric pathogens with PCR. Biomed. Environ. Sci. 17:109120.
33. Li, Y.,, E. Frey,, A. M. Mackenzie, and , B. B. Finlay. 2000. Human response to Escherichia coli O157:H7 infection: antibodies to secreted virulence factors. Infect. Immun. 68:50905095.
34. Losonsky, G. A.,, C. Ferreccio,, K. L. Kotloff,, S. Kaintuck,, J. B. Robbins, and , M. M. Levine. 1987. Development and evaluation of an enzyme-linked immunosorbent assay for serum Vi antibodies for detection of chronic Salmonella typhi carriers. J. Clin. Microbiol. 25:22662269.
35. Losonsky, G. A.,, Y. Lim,, P. Motamedi,, L. E. Comstock,, J. A. Johnson,, J. G. Morris, Jr.,, C. O. Tacket,, J. B. Kaper, and , M. M. Levine. 1997. Vibriocidal antibody responses in North American volunteers exposed to wild-type or vaccine Vibrio cholerae O139: specificity and relevance to immunity. Clin. Diagn. Lab. Immunol. 4:264269.
36. Ludwig, K.,, E. Grabhorn,, M. Bitzan,, C. Bobrowski,, M. J. Kemper,, I. Sobottka,, R. Laufs,, H. Karch, and , D. E. Muller-Wiefel. 2002. Saliva IgM and IgA are a sensitive indicator of the humoral immune response to Escherichia coli O157 lipopolysaccharide in children with enteropathic hemolytic uremic syndrome. Pediatr. Res. 52:307313.
37. Martinez-Govea, A.,, J. Ambrosio,, L. Gutierrez-Cogco, and , A. Flisser. 2001. Identification and strain differentiation of Vibrio cholerae by using polyclonal antibodies against outer membrane proteins. Clin. Diagn. Lab. Immunol. 8:768771.
38. Massi, M. N.,, T. Shirakawa,, A. Gotoh,, A. Bishnu,, M. Hatta, and , M. Kawabata. 2003. Rapid diagnosis of typhoid fever by PCR assay using one pair of primers from flagellin gene of Salmonella typhi. J. Infect. Chemother. 9:233237.
39. Nardiello, S.,, T. Pizzella,, M. Russo, and , B. Galanti. 1984. Serodiagnosis of typhoid fever by enzyme-linked immunosorbent assay determination of anti-Salmonella typhi lipopolysaccharide antibodies. J. Clin. Microbiol. 20:718721.
40. Nataro, J. P., and , J. B. Kaper. 1998. Diarrheagenic Escherichia coli. Clin. Microbiol. Rev. 11:142201.
41. Nguyen, T. V.,, P. Le Van,, C. Le Huy,, K. N. Gia, and , A. Weintraub. 2005. Detection and characterization of diarrheagenic Escherichia coli from young children in Hanoi, Vietnam. J. Clin. Microbiol. 43:755760.
42. Olsen, S. J.,, J. Pruckler,, W. Bibb,, T. M. Nguyen,, M. T. Tran,, T. M. Nguyen,, S. Sivapalasingam,, A. Gupta,, T. P. Phan,, T. C. Nguyen,, V. C. Nguyen,, D. C. Phung, and , E. D. Mintz. 2004. Evaluation of rapid diagnostic tests for typhoid fever. J. Clin. Microbiol. 42:18851889.
43. Paton, A. W., and , J. C. Paton. 2003. Detection and characterization of STEC in stool samples using PCR, p. 4554. In D. Philpott and , F. Ebel (ed.), E. coli Shiga Toxin Methods and Protocols. Humana Press Inc., Totowa, N.J.
44. Paton, J. C., and , A. W. Paton. 1998. Pathogenesis and diagnosis of Shiga toxin-producing Escherichia coli infections. Clin. Microbiol. Rev. 11:450479.
45. Paton, J. C., and , A. W. Paton. 2003. Methods for detection of STEC in humans, p. 926. In D. Philpott and , F. Ebel (eds.), E. coli Shiga Toxin Methods and Protocols. Humana Press Inc., Totowa, N.J.
46. Qadri, F.,, A. Chowdhury,, J. Hossain,, K. Chowdhury,, T. Azim,, T. Shimada,, K. M. Islam,, R. B. Sack, and , M. J. Albert. 1994. Development and evaluation of rapid monoclonal antibody-based coagglutination test for direct detection of Vibrio cholerae O139 synonym Bengal in stool samples. J. Clin. Microbiol. 32:15891590.
47. Quiroga, T.,, M. Goycoolea,, R. Tagle,, F. Gonzalez,, L. Rodriguez, and , L. Villarroel. 1992. Diagnosis of typhoid fever by two serologic methods. Enzyme-linked immunosorbent assay of antilipopolysaccharide of Salmonella typhi antibodies and Widal test. Diagn. Microbiol. Infect. Dis. 15:651656.
48. Reischl, U.,, M. T. Youssef,, H. Wolf,, E. Hyytia-Trees, and , N. A. Strockbine. 2004. Real-time fluorescence PCR assays for detection and characterization of heat-labile I and heat-stable I enterotoxin genes from enterotoxigenic Escherichia coli.J. Clin. Microbiol. 42:40924100.
49. Ristaino, P. A.,, M. M. Levine, and , C. R. Young. 1983. Improved GM1-enzyme-linked immunosorbent assay for detection of Escherichia coli heat-labile enterotoxin. J. Clin. Microbiol. 18:808815.
50. Sanches, M. I.,, R. Keller,, E. L. Hartland,, D. M. Figueired,, M. Batchelor,, M. B. Martinez,, G. Dougan,, M. M. Careiro-Sampaio,, G. Frankel, and , L. R. Trabulsi. 2000. Human colostrum and serum contain antibodies reactive to the intimin-binding region of the enteropathogenic Escherichia coli translocated intimin receptor. J. Pediatr. Gastroenterol. Nutr. 30:7377.
51. Sanchez-Jimenez, M. M., and , N. Cardona-Castro. 2004. Validation of a PCR for diagnosis of typhoid fever and salmonellosis by amplification of the hilA gene in clinical samples from Colombian patients. J. Med. Microbiol. 53:875878.
52. Steinsland, H.,, P. Valentiner-Branth,, H. M. Grewal,, W. Gaastra,, K. K. Molbak, and , H. Sommerfelt. 2003. Development and evaluation of genotypic assays for the detection and characterization of enterotoxigenic Escherichia coli. Diagn. Microbiol. Infect. Dis. 45:97105.
53. Tacket, C. O.,, M. F. Pasetti,, M. B. Sztein,, S. Livio, and , M. M. Levine. 2004. Immune responses to an oral typhoid vaccine strain that is modified to constitutively express Vi capsular polysaccharide. J. Infect. Dis. 190:565570.
54. Thompson, M. R.,, R. L. Jordan,, M. A. Luttrell,, H. Brandwein,, J. B. Kaper,, M. M. Levine, and , R. A. Giannella. 1986. Blinded, two-laboratory comparative analysis of Escherichia coli heat-stable enterotoxin production by using monoclonal antibody enzyme-linked immunosorbent assay, radioimmunoassay, suckling mouse assay, and gene probes. J. Clin. Microbiol. 24:753758.
55. Venkatesan, M. M.,, J. M. Buysse, and , D. J. Kopecko. 1989. Use of Shigella flexneri ipaC and ipaH gene sequences for the general identification of Shigella spp. and enteroin-vasive Escherichia coli. J. Clin. Microbiol. 27:26872691.
56. Vidal, R.,, M. Vidal,, R. Lagos,, M. Levine, and , V. Prado. 2004. Multiplex PCR for diagnosis of enteric infections associated with diarrheagenic Escherichia coli. J. Clin. Microbiol. 42:17871789.

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