Chapter 54 : Serologic and Molecular Diagnosis of Infection and Eradication

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The CagA protein, which is highly immunogenic, is one of the most widely studied virulence factors of . The cagA gene is one of the genes in the cag pathogenicity island. A variety of techniques are available for the diagnosis of infection. In this chapter the tests are discussed generically rather than listed specifically. In an attempt to eliminate the need for venipuncture, diagnostic tests have been developed for the detection of IgG and IgA antibodies in saliva and urine. Studies using urine-based enzyme-linked immunosorbent assay (ELISA) for detection of IgG antibodies against have been conducted. The most important factor in using immunological methods for identifying VacA-specific antibodies by ELISA is the antigen preparation used to detect circulating antibodies. The availability of recombinant VacA protein will help to make the VacA ELISA more widely available. In summary, ELISAs for detection of -specific antibodies are sensitive, specific, and cost-effective in untreated individuals. The major limitation of such tests is the lack of a rapid, clear serologic response to eradication of bacterial infection. Molecular tests have been developed which preclude culture and allow direct, sensitive detection of and specific virulence factors thereof in a variety of biological specimens. Much work remains to be done to standardize molecular assays so that they can be applied generally to the diagnosis of infection and eradication.

Citation: Dunn B, Phadnis S. 2006. Serologic and Molecular Diagnosis of Infection and Eradication, p 462-467. In Detrick B, Hamilton R, Folds J (ed), Manual of Molecular and Clinical Laboratory Immunology, 7th Edition. ASM Press, Washington, DC. doi: 10.1128/9781555815905.ch54

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Antimicrobial Susceptibility Testing
Enzyme-Linked Immunosorbent Assay
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Diagnostic tests for detection of

Citation: Dunn B, Phadnis S. 2006. Serologic and Molecular Diagnosis of Infection and Eradication, p 462-467. In Detrick B, Hamilton R, Folds J (ed), Manual of Molecular and Clinical Laboratory Immunology, 7th Edition. ASM Press, Washington, DC. doi: 10.1128/9781555815905.ch54

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