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Chapter 55 : Detection of Antibodies to

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Abstract:

Determination of the levels of antibody to was one of the first methods used to diagnose Legionnaires’ disease and was especially valuable when culture diagnosis was at a primitive stage. This chapter describes antibody testing because it is still very widely used and is of major importance for epidemiologic and research studies. Patients infected with some serogroups may not demonstrate antibody to other serogroups. About 40% of people who eventually seroconvert have agglutinating antibodies detected during the first week of infection. Detection of specific IgA antibodies may also increase the sensitivity and specificity of the enzyme-linked immunosorbent assay (ELISA). The sensitivity of the kits for measurement of antibody to SG2 to SG6 is unknown, but they do appear to be reasonably sensitive in comparison to the indirect immunofluorescent-antibody (IFA) method (70 to 90%) for the estimation of SG1 antibodies. Cross-reactive antibody is occasionally found in patients with non-Legionella infections. Up to 25% of people with enteritis have cross-reactive antibodies to . However, as many as 10% of patients with tuberculosis had cross-reactive antibodies to other serogroups and species. It is unclear whether this absorption removes only nonspecific antibodies and what criteria should be used to evaluate the serologic results of testing before and after the use of this blocking antigen. Pontiac fever is an acute, nonfatal febrile illness associated with but not proven to be caused by it.

Citation: Edelstein P. 2006. Detection of Antibodies to , p 468-476. In Detrick B, Hamilton R, Folds J (ed), Manual of Molecular and Clinical Laboratory Immunology, 7th Edition. ASM Press, Washington, DC. doi: 10.1128/9781555815905.ch55

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Enzyme-Linked Immunosorbent Assay
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References

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Tables

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TABLE 1

strains used for IFA antigens

Citation: Edelstein P. 2006. Detection of Antibodies to , p 468-476. In Detrick B, Hamilton R, Folds J (ed), Manual of Molecular and Clinical Laboratory Immunology, 7th Edition. ASM Press, Washington, DC. doi: 10.1128/9781555815905.ch55

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