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Chapter 56 : Immunologic Methods for Diagnosis of Spirochetal Diseases

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Abstract:

Syphilis is the most common spirochetal disease in the United States, with the most recent estimate being 32,871 newly reported cases in 2002. For the spirochetal diseases, direct dark-field microscopic examination often provides an immediate means of presumptive diagnosis. The direct fluorescent-antibody test for (DFA-TP) examination of tissue and body fluids is particularly valuable in the diagnosis of congenital syphilis. Subtyping for done at the Centers for Disease Control and Prevention is based on two genes that exhibit intrastrain variability, the genes encoding the acidic repeat protein (arp) and the repeat (tpr) protein. A diagnosis of leptospirosis should also be considered in cases of unexplained jaundice, aseptic meningitis, and fever of unknown origin. Definitive diagnosis of leptospirosis is made by isolation of leptospires from tissue or body fluids, but leptospires grow slowly on initial isolation and culture is therefore often not useful in patient management. The diagnostic tests discussed in this chapter are the microscopic agglutination test (MAT), which is the standard against which all other serologic tests for leptospirosis are evaluated, and two screening tests, the immunoglobulin M (IgM) dot enzyme-linked immunosorbent assay (ELISA) and the IgM ELISA. The MAT detects agglutinating antibodies and is considered the standard reference test for the diagnosis of leptospirosis.

Citation: Pope V, Ari M, Schriefer M, Levett P. 2006. Immunologic Methods for Diagnosis of Spirochetal Diseases, p 477-492. In Detrick B, Hamilton R, Folds J (ed), Manual of Molecular and Clinical Laboratory Immunology, 7th Edition. ASM Press, Washington, DC. doi: 10.1128/9781555815905.ch56

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FIGURE 1

in a Giemsa-stained human peripheral blood smear. Magnification, × 1,000.

Citation: Pope V, Ari M, Schriefer M, Levett P. 2006. Immunologic Methods for Diagnosis of Spirochetal Diseases, p 477-492. In Detrick B, Hamilton R, Folds J (ed), Manual of Molecular and Clinical Laboratory Immunology, 7th Edition. ASM Press, Washington, DC. doi: 10.1128/9781555815905.ch56
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References

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1. Bajani, M. D.,, D. A. Ashford,, S. L. Bragg,, C. W. Woods,, T. Aye,, R. A. Spiegel,, B. D. Plikaytis,, B. A. Perkins,, M. Phelan,, P. N. Levett, and , R. S. Weyant. 2003. Evaluation of four commercially available rapid serologic tests for diagnosis of leptospirosis. J. Clin. Microbiol. 41:803809.
2. Bal, A. E.,, C. Gravekamp,, R. A. Hartskeerl,, J. De Meza-Brewster,, H. Korver, and , W. J. Terpstra. 1994. Detection of leptospires in urine by PCR for early diagnosis of leptospirosis. J. Clin. Microbiol. 32:18941898.
3. Baranton, G., and , I. G. Old. 1995. The spirochaetes: a different way of life. Bull. Inst. Pasteur 93:6395.
4. Brenner, D. J.,, A. F. Kaufmann,, K. R. Sulzer,, A. G. Steigerwalt,, F. C. Rogers, and , R. S. Weyant. 1999. Further determination of DNA relatedness between serogroups and serovars in the family Leptospiraceae with a proposal for Leptospira alexanderi sp. nov. and four new Leptospira genomospecies. Int. J. Syst. Bacteriol. 49:839858.
5. Brown, P. D.,, C. Gravekamp,, D. G. Carrington,, H. Van de Kemp,, R. A. Hartskeerl,, C. N. Edwards,, C. O. R. Everard,, W. J. Terpstra, and , P. N. Levett. 1995. Evaluation of the polymerase chain reaction for early diagnosis of leptospirosis. J. Med. Microbiol. 43:110114.
6. Centurion-Lara, A.,, C. Godornes,, C. Castro,, W. C. van Voorhis, and , S. A. Lukehart. 2000. The tprK gene is heterogeneous among Treponema pallidum strains and has multiple alleles. Infect. Immun. 68:824831.
7. Cumberland, P. C.,, C. O. R. Everard, and , P. N. Levett. 1999. Assessment of the efficacy of the IgM enzyme-linked immunosorbent assay (ELISA) and microscopic agglutination test (MAT) in the diagnosis of acute leptospirosis. Am. J. Trop. Med. Hyg. 61:731734.
8. Cumberland, P. C.,, C. O. R. Everard,, J. G. Wheeler, and , P. N. Levett. 2001. Persistence of anti-leptospiral IgM, IgG and agglutinating antibodies in patients presenting with acute febrile illness in Barbados 1979-1989. Eur. J. Epidemiol. 17:601608.
9. Dennis, D. T., and , N. B. Hayes. 2004. Relapsing fever, p. 991995. In D. L. Kasper,, E. Braunwald,, A. S. Fauci,, S. L. Hauser,, D. L. Longo, and , J. L. Jameson (ed.), Harrison’s Principles of Internal Medicine, 16th ed. McGraw Hill, New York, N.Y.
10. Dworkin, M. S.,, P. C. Shoemaker,, C. L. Fritz,, M. E. Dowell, and , D. E. Anderson, Jr. 2002. The epidemiology of tick-borne relapsing-fever in the United States. Am. J. Trop. Med. Hyg. 66:753758.
11. Faine, S.,, B. Adler,, C. Bolin, and , P. Perolat. 1999. Leptospira and Leptospirosis, 2nd ed. MedSci, Melbourne, Australia.
12. Fritz, C. L.,, L. R. Bronson,, C. R. Smith,, M. E. Schriefer,, J. R. Tucker, and , T. G. Schwan. 2004. Isolation and characterization of Borrelia hermsii associated with two foci of tick-borne relapsing-fever in California. J. Clin. Microbiol. 42:11231128.
13. Gravekamp, C.,, H. Van de Kemp,, M. Franzen,, D. Carrington,, G. J. Schoone,, G. J. Van Eys,, C. O. Everard,, R. A. Hartskeerl, and , W. J. Terpstra. 1993. Detection of seven species of pathogenic leptospires by PCR using two sets of primers. J. Gen. Microbiol. 139:16911700.
14. Harkin, K. R.,, Y. M. Roshto,, J. T. Sullivan,, T. J. Purvis, and , M. M. Chengappa. 2003. Comparison of polymerase chain reaction assay, bacteriologic culture, and serologic testing in assessment of prevalence or urinary shedding of leptospires in dogs. J. Am. Vet. Med. Assoc. 222:12301233.
15. Herrmann, J. L. 1993. Genomic techniques for identification of Leptospira strains. Pathol. Biol. 41:943950.
16. Holt, J. G.,, N. R. Krieg,, P. H. A. Sneath,, J. T. Staley, and , S. T. Williams (ed.). 1994. The spirochetes, p. 2737. In W. R. Hensyl (ed.), Bergey’s Manual of Systemic Bacteriology, 9th ed. The Williams & Wilkins Co., Baltimore, Md.
17. Hookey, J. V. 1993. Characterization of Leptospiraceae by 16S DNA restriction length polymorphisms. J. Gen. Microbiol. 139:16811689.
18. Ko, A. I.,, M. Galvao Reis,, C. M. Ribeiro Dourado,, W. D. Johnson,, L. W. Riley, and the Salvador Leptospirosis Study Group. 1999. Urban epidemic of severe leptospirosis in Brazil. Lancet 354:820825.
19. Larsen, S. A.,, V. Pope,, R. E. Johnson, and , E. J. Kennedy, Jr. (ed.). 1998. A Manual of Tests for Syphilis, 9th ed. American Public Health Association, Washington, D.C.
20. Levett, P. N. 2003. Leptospira and Leptonema, p. 929936. In P. R. Murray,, E. J. Baron,, J. H. Jorgensen,, M. A. Pfaller, and , R. H. Yolken (ed.), Manual of Clinical Microbiology, 8th ed. ASM Press, Washington, D.C.
21. Levett, P. N. 2001. Leptospirosis. Clin. Microbiol. Rev. 14:296326.
22. Levett, P. N., and , S. L. Branch. 2002. Evaluation of two enzyme-linked immunosorbent assay methods for detection of immunoglobulin M antibodies in acute leptospirosis. Am. J. Trop. Med. Hyg. 66:745748.
23. Levett, P. N.,, S. L. Branch,, C. U. Whittington,, C. N. Edwards, and , H. Paxton. 2001. Two methods for rapid serological diagnosis of acute leptospirosis. Clin. Diagn. Lab. Immunol. 8:349351.
24. Levett, P. N.,, R. E. Morey,, R. L. Galloway,, D. E. Turner,, A. G. Steigerwalt, and , L. W. Mayer. 2005. Detection of pathogenic leptospires by real-time quantitative PCR J. Med. Microbiol. 54:4549.
25. Liu, H.,, B. Rodes,, C.-Y. Chen, and , B. Steiner. 2001. New tests for syphilis: rational design of a PCR method for detection of Treponema pallidum in clinical specimens using unique regions of the DNA polymerase I gene. J. Clin. Microbiol. 39:19411946.
26. Merien, F.,, P. Amouriaux,, P. Perolat,, G. Baranton, and , I. Saint Girons. 1992. Polymerase chain reaction for detection of Leptospira spp. in clinical samples. J. Clin. Microbiol. 30:22192224.
27. Merien, F.,, G. Baranton, and , P. Pérolat. 1995. Comparison of polymerase chain reaction with microagglutination test and culture for diagnosis of leptospirosis. J. Infect. Dis. 172:281285.
28. Morgan, J.,, S. L. Bornstein,, A. M. Karpati,, M. Bruce,, C. A. Bolin,, C. C. Austin,, C. W. Woods,, J. Lingappa,, C. Langkop,, B. Davis,, D. R. Graham,, M. Proctor,, D. A. Ashford,, M. Bajani,, S. L. Bragg,, K. Shutt,, B. A. Perkins, and , J. W. Tappero. 2002. Outbreak of leptospirosis among triathlon participants and community residents in Springfield, Illinois, 1998. Clin. Infect. Dis. 34:15931599.
29. Orle, K. A.,, C. A. Gates,, D. H. Martin,, B. A. Body, and , J. B. Weiss. 1996. Simultaneous PCR detection of Haemophilus ducreyi, Treponema pallidum, and herpes simplex virus types 1 and 2 from genital ulcers. J. Clin. Microbiol. 34:4954.
30. Picken, R.N. 1992. Polymerase chain reaction primers and probes derived from flagellin gene sequences for specific detection of the agents of Lyme disease and North American relapsing-fever. J. Clin. Microbiol. 30:99114.
31. Pillay, A.,, H. Liu,, C. Y. Chen,, B. Holloway,, A. W. Sturm,, B. Steiner. and , S. A. Morse. 1998. Molecular subtyping of Treponema pallidum subspecies pallidum. Sex. Transm. Dis. 25:408414.
32. Porcella, S. F.,, S. J. Raffel,, M. E. Schrumph,, M. E. Schriefer,, D. T. Dennis, and , T.G. Schwan. 2000. Glycerophosphodiester phosphodiesterase (GlpQ) of Borrelia recurrentis and its use for serodiagnosis of louseborne relapsing-fever. J. Clin. Microbiol. 38:35613571.
33. Rich, S. M.,, S. A. Sawyer, and , A. G. Barbour. 2000. Antigen polymorphism in Borrelia hermsii, a clonal pathogenic bacterium. Proc. Natl. Acad. Sci. USA 98:1503815043.
34. Schmid, G. P. 1989. Epidemiology and clinical similarities of human spirochetal diseases. Rev. Infect. Dis. 2: 5146051469.
35. Sejvar, J.,, E. Bancroft,, K. Winthrop,, M. J. Bettinger,, M. Bajani,, S. Bragg,, K. Shutt,, R. Kaiser,, N. Marano,, T. Popovic,, J. Tappero,, D. Ashford,, L. Mascola,, D. Vugia,, B. Perkins, and , N. Rosenstein. 2003. Leptospirosis in “Eco-challenge” athletes, Malaysian Borneo, 2000. Emerg. Infect. Dis. 9:702707.
36. Smythe, L. D.,, I. L. Smith,, G. A. Smith,, M. F. Dohnt,, M. L. Symonds,, L. J. Barnett, and , D. B. McKay. 2002. A quantitative PCR (TaqMan) assay for pathogenic Leptospira spp. BMC Infect. Dis. 2:13.
37. Terpstra, W. J. 1992. Typing Leptospira from the perspective of a reference laboratory. Acta Leiden. 60:7987.
38. Van Dam, A. P.,, T. Van Gool,, J. C. F. M. Wetsteyn, and , J. Dankert. 1999. Tick-borne relapsing-fever imported from West Africa: diagnosis by quantitative buffy coat analysis and in vitro culture of Borrelia corcidurae. J. Clin. Microbiol. 37:20272030.
39. Wagenaar, J.,, R. L. Zuerner,, D. Alt, and , C. A. Bolin. 2000. Comparison of polymerase chain reaction assays with bacteriologic culture, immunofluorescence, and nucleic acid hybridization for detection of Leptospira borgpetersenii serovar hardjo in urine of cattle. Am. J. Vet. Res. 61:316320.
40. Winslow, W. E.,, D. J. Merry,, M. L. Pirc, and , P. L. Devine. 1997. Evaluation of a commercial enzyme-linked immunosorbent assay for detection of immunoglobulin M antibody in diagnosis of human leptospiral infection. J. Clin. Microbiol. 35:19381942.

Tables

Generic image for table
TABLE 1

Characteristics of the order

Citation: Pope V, Ari M, Schriefer M, Levett P. 2006. Immunologic Methods for Diagnosis of Spirochetal Diseases, p 477-492. In Detrick B, Hamilton R, Folds J (ed), Manual of Molecular and Clinical Laboratory Immunology, 7th Edition. ASM Press, Washington, DC. doi: 10.1128/9781555815905.ch56
Generic image for table
TABLE 2

Spirochetal diseases of humans

Citation: Pope V, Ari M, Schriefer M, Levett P. 2006. Immunologic Methods for Diagnosis of Spirochetal Diseases, p 477-492. In Detrick B, Hamilton R, Folds J (ed), Manual of Molecular and Clinical Laboratory Immunology, 7th Edition. ASM Press, Washington, DC. doi: 10.1128/9781555815905.ch56
Generic image for table
TABLE 3

Tests for diagnosis of spirochetal diseases

Citation: Pope V, Ari M, Schriefer M, Levett P. 2006. Immunologic Methods for Diagnosis of Spirochetal Diseases, p 477-492. In Detrick B, Hamilton R, Folds J (ed), Manual of Molecular and Clinical Laboratory Immunology, 7th Edition. ASM Press, Washington, DC. doi: 10.1128/9781555815905.ch56
Generic image for table
TABLE 4

Reporting results of the RPR card test on serum (quantitative test)

Citation: Pope V, Ari M, Schriefer M, Levett P. 2006. Immunologic Methods for Diagnosis of Spirochetal Diseases, p 477-492. In Detrick B, Hamilton R, Folds J (ed), Manual of Molecular and Clinical Laboratory Immunology, 7th Edition. ASM Press, Washington, DC. doi: 10.1128/9781555815905.ch56
Generic image for table
TABLE 5

Reporting system for TP-PA test

Citation: Pope V, Ari M, Schriefer M, Levett P. 2006. Immunologic Methods for Diagnosis of Spirochetal Diseases, p 477-492. In Detrick B, Hamilton R, Folds J (ed), Manual of Molecular and Clinical Laboratory Immunology, 7th Edition. ASM Press, Washington, DC. doi: 10.1128/9781555815905.ch56
Generic image for table
TABLE 6

PCR assays for diagnosis of leptospirosis in humans

Citation: Pope V, Ari M, Schriefer M, Levett P. 2006. Immunologic Methods for Diagnosis of Spirochetal Diseases, p 477-492. In Detrick B, Hamilton R, Folds J (ed), Manual of Molecular and Clinical Laboratory Immunology, 7th Edition. ASM Press, Washington, DC. doi: 10.1128/9781555815905.ch56

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