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Chapter 59 : Immunologic and Molecular Diagnostic Methods

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Abstract:

This chapter discusses the advantages and disadvantages of current molecular and serologic diagnostic techniques for mycoplasmal infection and ureaplasmal infection. Established approaches to the detection of , , , and infections are discussed. A majority of the discussion in the chapter focuses on , but many of the same principles and limitations of detection also apply to the other members of the . In addition, the extent to which newer commercial serologic assays for will cross-react with or other mycoplasma was not established with certainty, but this seems less likely to be a problem than with complement fixation (CF). An in-depth discussion of various types of commercial antibody assays and a comparative analysis of them based on published studies is available in a recent review. Perhaps more than for any other mycoplasma, molecular biology-based techniques have played the critical and indeed defining role in diagnosis of infections. The molecular biology-based techniques and considerations are essentially the same as for the other mycoplasmal species. The most common target gene is 16S rRNA, and both multiplex PCR and real-time PCR assays have been developed. Because of the relative ease of cultivation, standardization and validation of the PCR assays can be more easily achieved.

Citation: Waites K, Brown M, Simecka J. 2006. Immunologic and Molecular Diagnostic Methods, p 510-517. In Detrick B, Hamilton R, Folds J (ed), Manual of Molecular and Clinical Laboratory Immunology, 7th Edition. ASM Press, Washington, DC. doi: 10.1128/9781555815905.ch59

Key Concept Ranking

Nucleic Acid Amplification Techniques
0.7601588
Multiplex Real-Time PCR
0.5635296
Real-Time PCR
0.41666672
0.7601588
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References

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Tables

Generic image for table
TABLE 1

Major commercial serologic test kits for in United States

Citation: Waites K, Brown M, Simecka J. 2006. Immunologic and Molecular Diagnostic Methods, p 510-517. In Detrick B, Hamilton R, Folds J (ed), Manual of Molecular and Clinical Laboratory Immunology, 7th Edition. ASM Press, Washington, DC. doi: 10.1128/9781555815905.ch59
Generic image for table
TABLE 2

Gene target used in nucleic acid amplification assays

Citation: Waites K, Brown M, Simecka J. 2006. Immunologic and Molecular Diagnostic Methods, p 510-517. In Detrick B, Hamilton R, Folds J (ed), Manual of Molecular and Clinical Laboratory Immunology, 7th Edition. ASM Press, Washington, DC. doi: 10.1128/9781555815905.ch59
Generic image for table
TABLE 3

Examples of PCR-based assays and gene targets for detection of urogenital mycoplasmas

Citation: Waites K, Brown M, Simecka J. 2006. Immunologic and Molecular Diagnostic Methods, p 510-517. In Detrick B, Hamilton R, Folds J (ed), Manual of Molecular and Clinical Laboratory Immunology, 7th Edition. ASM Press, Washington, DC. doi: 10.1128/9781555815905.ch59

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