Chapter 70 : Herpes Simplex Virus

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Human infections with herpes simplex virus (HSV) type 1 (HSV-1) and HSV-2 are ubiquitous throughout the world. The most frequent manifestation of primary HSV-1 infection is gingovostomatitis in young children, with pharyngitis occurring more commonly in adolescents. The seroprevalence of HSV-2 has increased more than 30% in recent decades, and the majority of seropositive persons are unaware that they are infected. Humans produce antibody to the structural components of the virus, the envelope, capsid, and internal proteins, as well as soluble nonvirion antigens specified by the virus. The only human herpesvirus that shows significant cross-reactivity with HSV-1 and HSV-2 is varicella-zoster virus (VZV). Paired serum samples from patients with recent VZV infection who have preexisting HSV antibody may sometimes show a rise in the level of antibodies to HSV-1 and HSV-2 in nonspecific tests. It is advisable to use several different HSV-susceptible cell lines, such as HEp-2, Vero-E6, and RD, to maximize the chance for successful culture. Enzyme immunoassay (EIA), indirect hemagglutination assay, immunoblotting, immunofluorescence assay (IFA), and the neutralization (NT) test have had the widest application for serodiagnosis and serosurveys. Radioimmunoassays are infrequently used because current EIA and immunoblotting procedures fulfill the same purpose and avoid the problem of using and disposing of radioisotopes. In studies directly contrasting the performance of PCR with culture, direct fluorescent-antibody assay, and other techniques, PCR is invariably more sensitive and at least comparably specific.

Citation: Schmid D. 2006. Herpes Simplex Virus, p 626-630. In Detrick B, Hamilton R, Folds J (ed), Manual of Molecular and Clinical Laboratory Immunology, 7th Edition. ASM Press, Washington, DC. doi: 10.1128/9781555815905.ch70

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Indirect Enzyme-Linked Immunosorbent Assay
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1. Arvin, A. M., and , C. G. Prober. 1999. Herpes simplex viruses, p. 878887. In P. R. Murphy,, E. J. Baron,, M. A. Pfaller,, F. C. Tenover, and , R. H. Yolken (ed.), Manual of Clinical Microbiology, 7th ed. American Society for Microbiology, Washington, D.C.
2. Ashley, R. L. 1998. Type-specific antibodies to HSV-1 and -2: review of methodology. Herpes 5:3338.
3. Ashley, R. L. 2001. Sorting out the new HSV type specific antibody tests. Sex. Transm. Infect. 77:232237.
4. Ashley, R. L., and , L. Corey. 1989. Herpes simplex virus, p. 265317. In N. J. Schmidt and , R. W. Emmons (ed.), Diagnostic Procedures for Viral, Rickettsial and Chlamydial Infections, 6th ed. American Public Health Association, Washington, D.C.
5. Ashley, R. L.,, J. Dalessio,, J. Dragavon,, L. A. Koutsky,, F. Lee,, A. J. Nahmias,, C. F. Stevens,, K. K. Holmes, and , L. Corey. 1993. Underestimation of HSV-2 seroprevalence in a high-risk population by microneutralization assay. Sex. Transm. Dis. 20:230235.
6. Ashley, R. L.,, M. Eagleton, and , N. Pheiffer. 1999. Ability of a rapid serology test to detect seroconversion to herpes simplex virus type 2 glycoprotein G soon after infection. J. Clin. Microbiol. 37:16321633.
7. Ashley, R. L.,, J. Militoni,, F. Lee,, A. Nahmias, and , L. Corey. 1988. Comparison of Western blot (immunoblot) and glycoprotein G-specific immunodot enzyme assay for detecting antibodies to herpes simplex virus types 1 and 2 in human sera. J. Clin. Microbiol. 26:662667.
8. Athmanathan, S.,, S. B. Reddy,, R. Nutheti, and , G. N. Rao. 2002. Comparison of an immortalized human corneal epithelial cell line with Vero cells in the isolation of herpes simplex virus-1 for the laboratory diagnosis of herpes simplex keratitis. BMC Ophthalmol. 2:3.
9. Bergström, T., and , F. Trybala. 1996. Antigenic differences between HSV-1 and HSV-2 glycoproteins and their importance for type-specific serology. Intervirology 39:176184.
10. Berstein, M. T., and , J. A. Stewart. 1971. Method for typing antisera to herpesvirus hominis by indirect hemaggluti-nation inhibition. Appl. Microbiol. 21:680684.
11. Brown, Z. A.,, S. Selke,, J. Zeh,, J. Kopelman,, A. Maslow,, R. L. Ashley,, D. H. Watts,, S. Berry,, M. Herd, and , L. Corey. 1997. The acquisition of herpes simplex virus during pregnancy. N. Engl. J. Med. 337:509515.
12. Burrows, J.,, A. Nitsche,, B. Bayly,, F. Walker,, G. Higgins, and , T. Kok. 2002. Detection and subtyping of herpes simplex virus in clinical samples by Light-Cycler PCR, enzyme immunoassay and cell culture. BMC Microbiol. 2:12.
13. Cremer, N. E.,, C. K. Cossen,, C. V. Hanson, and , G. R. Shell. 1982. Evaluation and reporting of enzyme immunoassay determinations of antibody to herpes simplex virus in sera and cerebrospinal fluid. J. Clin. Microbiol. 15:815823.
14. Damon, I.,, L. Rotz,, J. Seward, and , J. Hughes. 2003. Comment on: Hanrahan, et al. 2003. A smallpox false alarm. N. Engl. J. Med. 348:467468. N. Engl. J. Med. 348:468.
15. Eis-Hilbinger, A. M.,, M. Däumer,, B. Matz, and , K. F. Schneweis. 1999. Evaluation of three glycoprotein G2-based enzyme immunoassays for detection of antibodies to herpes simplex virus type 2 in human sera. J. Clin. Microbiol. 37:12421246.
16. Fleming, D. T.,, G. W. McQuillan,, R. F. Johnson,, A. J. Nahmias,, S. O. Aral,, F. K. Lee, and , M. F. St. Louis. 1997. Herpes simplex virus type 2 in the United States, 1976 to 1994. N. Engl. J. Med. 337:11051111.
17. Groen, J.,, G. Van Dijk,, H. G. M. Niesters,, W. I. Van der Meijden, and , A. D. M. F. Osterhaus. 1998. Comparison of two enzyme-linked immunosorbent assays and one rapid immunoblot assay for detection of herpes simplex virus type-2-specific antibodies in serum. J. Clin. Microbiol. 36:845847.
18. Hanrahan, J. A.,, M. Jakubowycz, and , R. D. Bryan. 2003. A smallpox false alarm. N. Engl. J. Med. 348:467468.
19. Hashido, M.,, S. Inouye, and , T. Kawana. 1997. Differentiation of primary from nonprimary genital herpes infections by a herpes simplex virus-specific immunoglobulin G avidity test. J. Clin. Microbiol. 35:17661768.
20. Hashido, M., and , T. Kawana. 1997. Herpes simplex virus-specific IgM, IgA, and IgG subclass antibody responses in primary and nonprimary genital herpes patients. Microbiol. Immunol. 41:415420.
21. Hashido, M.,, F. K. Lee,, S. Inouye, and , T. Kawana. 1997. Detection of herpes simplex virus type-specific antibodies by an enzyme-linked immunosorbent assay based on glycoprotein G. J. Med. Virol. 53:319323.
22. Ho, D. W. T.,, P. R. Field,, F. Sjogren-Jansson,, S. Jeansson, and , A. L. Cunningham. 1992. Indirect ELISA for the detection of HSV-2 specific IgG and IgM antibodies with glycoprotein G (gG-2). J. Virol. Methods 36:249264.
23. Koenig, M.,, K. S. Reynolds,, W. Aldous, and , M. Hickman. 2001. Comparison of Light-Cycler PCR, enzyme immunoassay, and tissue culture for detection of Herpes Simplex Virus. Diagn. Microbiol. Infect. Dis. 40:107110.
24. Kulhanjian, J. A.,, V. Soroush,, D. S. Au,, R. N. Bronzan,, L. L. Yasukawa,, L. F. Weylman,, A. M. Arvin, and , C. G. Prober. 1992. Identification of women at unsuspected risk of primary infection with herpes simplex virus type 2 during pregnancy. N. Engl. J. Med. 326:916920.
25. Lee, F. K.,, R. M. Coleman,, L. Pereira,, P. D. Bailey,, M. Tatsuno, and , A. J. Nahmias. 1985. Detection of herpes simplex virus type 2-specific antibody with glycoprotein G. J. Clin. Microbiol. 22:641644.
26. Lee, F. K.,, L. Pereira,, C. Griffin,, F. Reid, and , A. Nahmias. 1986. A novel glycoprotein for detection of herpes simplex virus type 1-specific antibodies. J. Virol. Methods 14:111118.
27. Markoulatos, P.,, A. Georgopoulou,, C. Kotsovassilis,, P. Karabogia-Karaphillides, and , N. Spyrou. 2000. Detection and typing of HSV-1, HSV-2, and VZV by a multiplex polymerase chain reaction. J. Clin. Lab. Anal. 14:214219.
28. Monteyne, P.,, F. Albert,, B. Weissbrich,, F. Zardini,, M. Ciardi,, G. M. Cleator, and , C. J. M. Sindic. 1997. The detection of intrathecal synthesis of anti-herpes simplex IgG antibodies: comparison between an antigen-mediated immunoblotting technique and antibody index calculations. J. Med. Virol. 53:324331.
29. Nicoll, S.,, A. Brass, and , H. A. Cubie. 2001. Detection of herpes viruses in clinical samples using real-time PCR. J. Virol. Methods 96:2531.
30. O’Sullivan, C. E.,, A. J. Aksamit,, J. R. Harrington,, W. S. Harmsen,, P. S. Mitchell, and , R. Patel. 2003. Clinical spectrum and laboratory characteristics associated with detection of herpes simplex virus DNA in cerebrospinal fluid. Mayo Clin. Proc. 78:13471352.
31. Sánchez-Martinez, D.,, D. S. Schmid,, W. Whittington,, D. Brown,, W. C. Reeves,, S. Chatterjee,, R. J. Whitley, and , P. F. Pellett. 1991. Evaluation of a test based on baculovirus-expressed glycoprotein G for detection of herpes simplex virus type-specific antibodies. J. Infect. Dis. 164:11961199.
32. Schmid, D. S.,, D. R. Brown,, R. Nisenbaum,, R. L. Burke,, D. Alexander,, R. Ashley,, P. F. Pellett, and , W. C. Reeves. 1999. Limits in the reliability of glycoprotein G-based type-specific serologic assays for herpes simplex virus types 1 and 2. J. Clin. Microbiol. 37:376379.
33. Slomka, M. J.,, R. L. Ashley,, F. M. Cowan,, A. Cross, and , D. W. Brown. 1995. Monoclonal antibody blocking tests for the detection of HSV-1 and HSV-2 specific humoral responses: comparison with Western blot assay. J. Virol. Methods 55:2735.
34. Tang, Y.-W.,, J. R. Hibbs,, K. R. Tau,, Q. Qian,, H. A. Skarhus,, T. F. Smith, and , D. H. Persing. 1999. Effective use of polymerase chain reaction for diagnosis of central nervous system infections. Clin. Infect. Dis. 29:803806.
35. Whitley, R. J. 1996. Herpes simplex viruses, p. 22992342. In B. N. Fields,, D. M. Knipe, and , P. M. Howley (ed.), Fields Virology, 3rd ed. Lippincott-Raven Publishers, Philadelphia, Pa.

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