Chapter 76 : Adenoviruses

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This chapter concentrates on serologic methods for diagnosis of human adenoviruses (HAds) infections and recent advances in molecular detection of viral nucleic acid. The most widely used methods for measuring HAd crossreactive, genus-specific antibodies of both the IgG and the IgM classes are enzyme immunoassay (EIA) and indirect immunofluorescence (IIF) assay (also called indirect fluorescent antibody assay). Complement fixation (CF), once used extensively, is the most standardized serologic test for diagnosis of HAd infection. A growing number of reports have linked HAds and acute myocarditis, with and without pericarditis, in infants and young children; sudden infant death associated with myocarditis; and idiopathic left ventricular dysfunction in adults. EIA is now one of the most automated tests in the clinical laboratory and, for the most part, uses the 96-well microplate format, automated plate washers, and readers that define experimental parameters and interpret results. The commercial conjugates for EIA that are currently available are antihuman immunoglobulins labeled with one of two types of enzyme, either horseradish peroxidase or alkaline phosphatase. Detection of viral nucleic acid in clinical materials is the most rapidly expanding diagnostic methodology in medicine, overtaking culture as the “gold standard” for diagnosis of infectious disease. The possibility of PCR contamination must also be evaluated. As with interpretation of serological test results, it is better to have the corroboration of additional methods of diagnosis if possible.

Citation: Crawford-Miksza L. 2006. Adenoviruses, p 677-683. In Detrick B, Hamilton R, Folds J (ed), Manual of Molecular and Clinical Laboratory Immunology, 7th Edition. ASM Press, Washington, DC. doi: 10.1128/9781555815905.ch76

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Restriction Fragment Length Polymorphism
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1. Akhtar, N.,, J. Ni,, C. Langston,, G. J. Demmler, and , J. A. Towbin. 1996. PCR diagnosis of viral pneumonitis from fixed-lung tissue in children. Biochem. Mol. Med. 58:6676.
2. Allard, A.,, B. Albinson, and , G. Wadell. 2001. Rapid typing of human adenoviruses by a general PCR combined with restriction endonuclease analysis. J. Clin. Microbiol. 39:498505.
3. Appenzeller, C.,, R. A. Ammann,, A. Duppenthaler,, M. Gorgievski-Hrisobo, and , C. Aebi. 2002. Serum C-reactive protein in children with adenovirus infection. Swiss Med. Wkly. 132:345350.
4. Avellon, A.,, P. Perez,, J. C. Aguilar,, R. O. de Lejarazu, and , J. E. Echevarria. 2001. Rapid and sensitive diagnosis of human adenovirus infections by a generic polymerase chain reaction. J. Virol. Methods 92:113120.
5. Barenfanger, J.,, C. Drake,, N. Leon,, T. Miller, and , T. Troutt. 2000. Clinical and financial benefits of rapid detection of respiratory viruses: an outcomes study. J. Clin. Microbiol. 38:28242828.
6. Bon, F. P. F.,, M. Dauvergne,, D. Tenenbaum,, H. Planson,, A. M. Petion,, P. Pothier, and , F. Kohli. 1999. Prevalence of group A rotavirus, human calicivirus, astrovirus, and adenovirus type 40 and 41 infections among children with acute gastroenteritis in Dijon, France. J. Clin. Microbiol. 37:30553058.
7. Boom, R.,, C. J. A. Sol,, M. M. M. Salimans,, C. L. Jansen,, P. M. E. Wertheim-van Dillen, and , J. van der Noordaa. 1990. Rapid and simple method for purification of nucleic acids. J. Clin. Microbiol. 28:495503.
8. Brandt, C. D.,, H. W. Kim,, A. J. Vargosko,, B. C. Jeffries,, J. O. Arrobio,, B. Rindge,, R. H. Parrott, and , R. M. Chanock. 1969. Infections in 18,000 infants and children in a controlled study of respiratory tract disease. I. Adenovirus pathogenicity in relation to serologic type and illness syndrome. Am. J. Epidemiol. 90:484500.
9. Cardosa, M. J.,, S. Krishnan,, P. H. Tio,, D. Perera,, S. C. Wong. 1999. Isolation of subgenus B adenovirus during a fatal outbreak of enterovirus 71-associated hand, foot and mouth disease in Sibu, Sarawak. Lancet 354:987991.
10. Centers for Disease Control. 1998. Civilian outbreak of adenovirus acute respiratory disease—South Dakota, 1997. Morb. Mortal. Wkly. Rep. 47:567570.
11. Chodosh, J.,, D. Miller,, W. G. Stroop, and , S. C. Pflugfelder. 1995. Adenovirus epithelial keratitis. Cornea 14:167174.
12. Crawford-Miksza, L. K., and , D. P. Schnurr. 1996. Analysis of 15 adenovirus hexon proteins reveals the location and structure of seven hypervariable regions containing serotype-specific residues. J. Virol. 70:18361844.
13. Crawford-Miksza, L. K., and , D. P. Schnurr. 1994. Quantitative colorimetric microneutralization assay for characterization of adenoviruses. J. Clin. Microbiol. 32:23312334.
14. Crawford-Miksza, L. K.,, R. N. Nang, and , D. P. Schnurr. 1999. Strain variation in adenovirus serotypes 4 and 7a causing acute respiratory disease. J. Clin. Microbiol. 37:11071112.
15. De Jong, J. C.,, A. G. Wermendol,, M. W. Verweij-Uijterwaal,, K. W. Slaterus,, P. Wertheim-Van Dillen,, G. J. J. Van Doornum,, S. H. Khoo, and , J. E. Hierholzer. 1999. Adenoviruses from human immunodeficiency virus-infected individuals, including two strains that represent new candidate serotypes Ad50 and Ad51 of species B1 and D, respectively. J. Clin. Microbiol. 37:39403945.
16. Erdman, D. D., and , J. F. Hierholzer. 1997. Adenoviruses, p. 661666. In N. R. Rose,, F. Conway de Macario,, J. D. Folds,, H. C. Lane, and , R. M. Nakamura (ed.), Manual of Clinical Laboratory Immunology, 5th ed. ASM Press, Washington, D.C.
17. Faix, D. J.,, H.-S. H. Houng,, J. C. Gaydos,, S.-K. S. Liu,, J. T. Connors,, X. Brown,, L. V. Asher,, D. W. Vaughn, and , L. N. Binn. 2004. Evaluation of a rapid quantitative diagnostic test for adenovirus type 4. Clin. Infect. Dis. 38:391397.
18. Fox, J. P., and , C. E. Hall. 1980. Viruses in Families, p. 294334. PSG Publishing, Littleton, Mass.
19. Gigliotti, F.,, W. T. Williams,, E. G. Hayden, and , J. O. Hendley. 1981. Etiology of acute conjunctivitis in children. J. Pediatr. 98:531536.
20. Gray, G. C.,, J. D. Callahan,, A. W. Hawksworth,, C. A. Fisher. and , J. C. Gaydos. 1999. Respiratory diseases among U.S. military personnel: countering emerging threats. Emerg. Infect. Dis. 5:379387.
21. Grayston, T. J.,, C. G. Loosli,, P. B. Johnston,, M. E. Smith, and , R. L. Woolridge. 1956. Neutralizing and complement fixing antibody response to adenovirus infection. J. Infect. Dis. 99:199206.
22. Gu, Z.,, S. W. Belzer,, C. S. Gibson,, M. J. Bankowski, and , R. T. Hayden. 2003. Multiplexed real-time PCR for quantitative detection of human adenovirus. J. Clin. Microbiol. 41:46314641.
23. Heim, A.,, C. Ebnet,, G. Harste, and , P. Pring-Akerblom. 2003. Rapid and quantitative detection of human adenovirus DNA by real-time PCR. J. Med. Virol. 70:228239.
24. Hierholzer, J. C.,, R. Wigand,, L. J. Anderson,, T. Adrian, and , J. W. M. Gold. 1988. Adenoviruses from patients with AIDS: a plethora of serotypes and a description of five new serotypes of subgenus D (43-47). J. Infect. Dis. 158:804813.
25. Hierholzer, J. C., and , P. G. Bingham. 1978. Vero micro-cultures for adenovirus neutralization tests. J. Clin. Microbiol. 7:499506.
26. Huebner, R. J.,, W. P. Rowe,, T. G. Ward,, R. H. Parrott, and , J. A. Bell. 1954. Adenoidal-pharyngeal-conjunctival agents: a newly recognized group of common viruses of the respiratory system. N. Engl. J. Med. 251:10771086.
27. Kawasaki, Y.,, M. Hosoya,, M. Katayose, and , H. Suzuki. 2002. Correlation between serum interleukin 6 and C-reactive protein concentrations in patients with adenoviral respiratory infection. Pediatr. Infect. Dis. J. 21:370374.
28. Kojaoghlanian, T.,, P. Flomenberg, and , M. S. Horwitz. 2003. The impact of adenovirus infection on the immuno-compromised host. Rev. Med. Virol. 13:155171.
29. Leruez-Ville, M.,, V. Minard,, F. Lacaille,, A. Buzyn,, E. Abachin,, S. Blanche,, F. Freymuth, and , C. Rouzioux. 2004. Real-time blood plasma polymerase chain reaction for management of disseminated adenovirus infection. Clin. Infect. Dis. 38:4552.
30. Li, Q.-G.,, A. Henningsson,, P. Juto,, F. Elgh, and , G. Wadell. 1999. Use of restriction fragment analysis and sequencing of a serotype-specific region to type adenovirus isolates. J. Clin. Microbiol. 37:844847.
31. Ludwig, S. L.,, J. F. Brundage,, P. W. Kelley,, R. N. Nang,, C. Towle,, D. P. Schnurr,, L. K. Crawford-Miksza, and , J. C. Gaydos. 1998. Prevalence of antibodies to adenovirus serotypes 4 and 7 among unimmunized US army trainees: results of a retrospective nationwide seroprevalence study. J. Infect. Dis. 178:17761778.
32. Martin, A. B.,, S. Webber,, F. J. Fricker,, R. Jaffe,, G. Demmler,, D. Kearney,, Y.-H. Zhang,, J. Bodurtha,, B. Gelb,, J. Ni,, J. T. Bricker, and , J. A. Towbin. 1994. Acute myocarditis: rapid diagnosis by PCR in children. Circulation 90:330339.
33. Pauschinger, M.,, N. E. Bowles,, F. J. Fuentes-Garcia,, V. Pham,, U. Kuehl,, P. L. Schwimmbeck,, H.-P. Schultheiss, and , J. A. Towbin. 1999. Detection of adenoviral genome in the myocardium of adult patients with idiopathic left ventricular dysfunction. Circulation 99:13481354.
34. Pehler-Harrington, K.,, M. Khanna,, C. R. Waters, and , K. J. Henrickson. 2004. Rapid detection and identification of human adenovirus species by Adenoplex, a multiplex PCR-enzyme hybridization assay. J. Clin. Microbiol. 42:40724076.
35. Raty, R.,, M. Kleemola,, K. Melen,, M. Stenvik, and , I. Julkunen. 1999. Efficacy of PCR and other diagnostic methods for the detection of respiratory adenoviral infections. J. Med. Virol. 59:6672.
36. Rocholl, C.,, K. Gerber,, J. Daly,, A. T. Pavia, and , C. L. Byington. 2004. Adenoviral infections in children: the impact of rapid diagnosis. Pediatrics 113:e51e56.
37. Rowe, W. P.,, R. J. Huebner,, L. K. Gilmore,, R. H. Parrott, and , T. J. Ward. 1953. Isolation of a cytopathogenic agent from human adenoids undergoing spontaneous degeneration in tissue culture. Proc. Soc. Exp. Biol. Med. 84:570573.
38. Rowe, W. P.,, R. J. Huebner,, L. K. Gilmore,, R. H. Parrott, and , T. J. Ward. 1957. Serotype composition of the adenovirus group. Proc. Soc. Exp. Biol. Med. 97:465470.
39. Sabin, C. A.,, G. S. Clewley,, J. R. Deayton,, A. Mocroft,, M. A. Johnson,, C. A. Lee,, J. E. McLaughlin, and , P. D. Griffiths. 1999. Shorter survival in HIV-positive patients with diarrhoea who excrete adenovirus from the GI tract. J. Med. Virol. 58:280285.
40. Saitoh-Inagawa, W.,, A. Oshima,, K. Aoki,, N. Itoh,, K. Isobe,, E. Uchio,, S. Ohno,, H. Nakajima,, K. Hata, and , H. Ishiko. 1996. Rapid diagnosis of adenoviral conjunctivitis by PCR and restriction fragment length polymorphism analysis. J. Clin. Microbiol. 34:21132116.
41. Sarantis, H.,, G. Johnson,, M. Brown,, M. Petric, and , R. Tellier. 2004. Comprehensive detection and serotyping of human adenoviruses by PCR and sequencing. J. Clin. Microbiol. 42:39633969.
42. Schnurr, D. P., and , M. E. Dondero. 1993. Two new candidate adenovirus serotypes. Intervirology 36:7983.
43. Shimizu, C.,, C. Rimbaud,, G. Cheron,, C. Rouzioux,, G. M. Lozinski,, A. Rao,, G. Stanway,, H. F. Krous, and , J. C. Burns. 1995. Molecular identification of viruses in sudden infant death associated with myocarditis and pericarditis. Pediatr. Infect. Dis. J. 14:584588.
44. Shimizu, H.,, D. P. Schnurr, and , J. C. Burns. 1994. Comparison of methods to detect enteroviral genome in frozen and fixed myocardium by polymerase chain reaction. Lab. Investig. 71:612616.
45. Swanson, P. D.,, G. Wadell,, A. Allard, and , J. C. Hierholzer. 2003. Adenoviruses, p. 14041417. In P. R. Murray,, E. J. Baron,, J. H. Jorgensen,, M. A. Pfaller, and , R. H. Yolken (ed.), Manual of Clinical Microbiology, 8th ed. ASM Press, Washington, D.C.
46. Tai, F.-H., and , J. T. Grayston. 1962. Adenovirus neutralizing antibodies in persons on Taiwan. Proc. Soc. Exp. Biol. Med. 109:881884.
47. Turner, P. C.,, A. S. Bailey,, R. J. Cooper, and , D. J. Morris. 1993. The polymerase chain reaction for detecting adenovirus DNA in formalin-fixed, paraffin-embedded tissue obtained post mortem. J. Infect. 27:4346.
48. Willcox, N., and , V. Mautner. 1976. Antigenic determinants of adenovirus capsids. J. Immunol. 116:1924.
49. Xu, W.,, M. C. McDonough, and , D. D. Erdman. 2000. Species-specific identification of human adenoviruses by a multiplex PCR assay. J. Clin. Microbiol. 38:41144120.
50. Yamadera, S.,, K. Yamashita,, M. Akatsuka,, N. Kato, and , S. Inouye. 1998. Trend of adenovirus type 7 infection, an emerging disease in Japan. Jpn. J. Med. Sci. Biol. 51:4351.
51. Yeh, H.-Y.,, N. Pieniazek,, D. Pieniazek,, H. Gelderblom, and , R. B. Luftig. 1994. Human adenovirus type 41 contains two fibers. Virus Res. 33:179198.


Generic image for table

Illnesses commonly associated with HAd infections

Citation: Crawford-Miksza L. 2006. Adenoviruses, p 677-683. In Detrick B, Hamilton R, Folds J (ed), Manual of Molecular and Clinical Laboratory Immunology, 7th Edition. ASM Press, Washington, DC. doi: 10.1128/9781555815905.ch76
Generic image for table

Ad serotypes grouped into species by hemagglutination and DNA homology

Citation: Crawford-Miksza L. 2006. Adenoviruses, p 677-683. In Detrick B, Hamilton R, Folds J (ed), Manual of Molecular and Clinical Laboratory Immunology, 7th Edition. ASM Press, Washington, DC. doi: 10.1128/9781555815905.ch76

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