Chapter 80 : Measles and Mumps

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Although both measles virus and mumps virus can be isolated in cell culture, isolation may be difficult due to the need to collect specimens very early in the infection process and to the slow proliferation and weak to absent production of cytopathogenic effect by these viruses in traditional tube cell cultures. Newer modified cell culture techniques involving detection of viral antigen in shell vial cultures have improved the sensitivity and speed of measles and mumps virus isolation. Molecular techniques, which do not rely on the presence of viable virus, can be used to detect viral RNA directly in clinical samples. The serologic approach, relying on detection of antibodies for confirmation of infection, has long been the most accessible and reliable diagnostic tool for confirming measles and mumps virus infections. The most widely used methods in measles and/or mumps antibody detection are enzyme immunoassay (EIA) and indirect immunofluorescence assay (IFA). Complement fixation (CF), hemagglutination inhibition (HI), neutralization (NT), and plaque reduction neutralization (PRN) are seldom used in diagnostic laboratories but may be available at reference or research facilities. A brief description of each of these methods is presented in this chapter. Molecular methods are more expensive than virus isolation but may provide a viable approach for confirming the presence of measles or mumps virus. The presence of measles virus RNA in clinical samples is evidence for current or very recent infection or vaccination.

Citation: Leland D. 2006. Measles and Mumps, p 707-711. In Detrick B, Hamilton R, Folds J (ed), Manual of Molecular and Clinical Laboratory Immunology, 7th Edition. ASM Press, Washington, DC. doi: 10.1128/9781555815905.ch80

Key Concept Ranking

Measles virus
Mumps virus
Rubella virus
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1. Afzal, M. A.,, J. Buchanan,, J. A. Dias,, M. Cordeiro,, M. L. Bentley,, C. A. Shorrock, and , P. D. Minor. 1997. RT-PCR based diagnosis and molecular characterisation of mumps viruses derived from clinical specimens collected during the 1996 mumps outbreak in Portugal. J. Med. Virol. 52:349353.
2. Afzal, M. A.,, A. D. Osterhaus,, S. L. Cosby,, L. Jin,, J. Beeler,, K. Takeuchi, and , H. Kawashima. 2003. Comparative evaluation of measles virus-specific RT-PCR methods through an international collaborative study. J. Med. Virol. 70:171176.
3. Bellini, W. J., and , R. F. Helfand. 2003. The challenges and strategies for laboratory diagnosis of measles in an international setting. J. Infect. Dis. 187(Suppl. 1):S283S290.
4. Bellini, W. J., and , P. A. Rota. 1999. Measles (rubeola) virus, p. 603621. In E. H. Lennette and , T. F. Smith (ed.), Laboratory Diagnosis of Viral Infections, 3rd ed. Marcel Dekker, Inc., New York, N.Y.
5. Black, F. L. 1997. Measles and mumps, p. 688692. In N. R. Rose,, E. C. de Macario,, J. D. Folds,, H. C. Lane, and , R. M. Nakamura (ed.), Manual of Clinical Laboratory Immunology, 5th ed. ASM Press, Washington, D.C.
6. Boriskin, Y. S.,, J. C. Booth, and , A. Yamada. 1993. Rapid detection of mumps virus by the polymerase chain reaction. J. Virol. Methods 42:2332.
7. Centers for Disease Control. 1989. Mumps prevention. Morb. Mortal. Wkly. Rep. 38:388391.
8. Centers for Disease Control and Prevention. 2004. Summary of notifiable diseases, US, 2002. [Online.] http://www.cdc.gov/mmwr/summary.html.
9. Centers for Disease Control and Prevention. 2003. Update: global measles control and mortality reduction—worldwide, 1991-2001. Morb. Mortal. Wkly. Rep. 52:471475.
10. Chakravarti, A.,, D. Rawat, and , S. Yadav. 2003. Whole blood samples as an alternative to serum for detection of immunity to measles virus by ELISA. Diagn. Microbiol. Infect. Dis. 47:563567.
11. Condorelli, F.,, G. Scalia,, A. Stivala,, R. Gallo,, A. Marino,, C. M. Battaglini, and , A. Castro. 1994. Detection of immunoglobulin G to measles virus, rubella virus, and mumps virus in serum samples and in microquantities of whole blood dried on filter paper. J. Virol. Methods 49:2536.
12. Dine, M. S.,, S. S. Hutchins,, A. Thomas,, I. Williams,, W. J. Bellini, and , S. C. Redd. 2004. Persistence of vaccine-induced antibody to measles 26-33 years after vaccination. J. Infect. Dis. 189(Suppl. 1):S123S130.
13. Glick, C.,, S. Feldman,, M. R. Norris, and , J. Butler. 1998. Measles, mumps, and rubella serology in premature infants weighing less than 1,000 grams. South. Med. J. 91:159160.
14. Helfand, R. F.,, H. L. Keyserling,, I. Williams,, A. Murray,, J. Mei,, C. Moscatiello,, J. Icenogle, and , W. J. Bellini. 2001. Comparative detection of measles and rubella IgM and IgG derived from filter paper blood and serum samples. J. Med. Virol. 65:751757.
15. Helfand, R. F.,, S. Kebede,, H. F. Gary, Jr.,, H. Beyene, and , W. J. Bellini. 1999. Timing of development of measles-specific immunoglobulin M and G after primary measles vaccination. Clin. Diagn. Lab. Immunol. 6:178180.
16. Hutchins, S. S.,, M. J. Papania,, R. Amler,, E. F. Maes,, M. Grabowsky,, K. Bromberg,, V. Glasglow,, T. Speed,, W. J. Bellini, and , W. A. Orenstein. 2004. Evaluation of the measles clinical case definition. J. Infect. Dis. 198(Suppl. 1):S153S159.
17. Kubar, A.,, M. Yapar,, B. Besirbellioglu,, I. Y. Avci, and , C. Guney. 2004. Rapid and quantitative detection of mumps virus RNA by one-step real-time RT-PCR. Diagn. Microbiol. Infect. Dis. 49:8388.
18. Mosquera, M.,, F. de Ory,, M. Moreno, and , J. F. Echevarria. 2002. Simultaneous detection of measles virus, rubella virus, and parvovirus B19 by using multiplex PCR. J. Clin. Microbiol. 40:111116.
19. Mubarak, H. S.,, S. Yukel,, O. M. Mustafa,, S. A. Ibrahim,, A. D. Osterhaus, and , R. L. de Swart. 2004. Surveillance of measles in the Sudan using filter paper blood samples. J. Med. Virol. 73:624630.
20. Nakayama, T.,, T. Mori,, S. Yamaguchi,, S. Sonoda,, S. Asamura,, R. Yamashita,, Y. Takeuchi, and , T. Urano. 1995. Detection of measles virus genome directly from clinical samples by reverse transcriptase-polymerase chain reaction and genetic variability. Virus Res. 35:116.
21. Permar, S. R.,, W. J. Moss,, J. J. Ryon,, M. Monze,, F. Cutts,, T. C. Quinn, and , D. F. Griffin. 2001. Prolonged measles virus shedding in human immunodeficiency virus-infected children, detected by reverse transcriptase-polymerase chain reaction. J. Infect. Dis. 183:532538.
22. Poggio, G. P.,, C. Rodriguez,, D. Cisterna,, M. C. Freire, and , J. Cello. 2000. Nested PCR for rapid detection of mumps virus in cerebrospinal fluid from patients with neurological diseases. J. Clin. Microbiol. 38:274278.
23. Riddell, M. A.,, J. A. Leydon,, M. G. Catton, and , H. A. Kelly. 2002. Detection of measles virus-specific immunoglobulin M in dried venous blood samples by using a commercial enzyme immunoassay J. Clin. Microbiol. 40:59.
24. Rose, N. R.,, F. Conway de Macario,, J. D. Folds,, H. C. Lane, and , R. M. Nakamura (ed.). 1997. Manual of Clinical Laboratory Immunology, 5th ed. ASM Press, Washington, D.C.
25. Rose, N. R.,, R. G. Hamilton, and , B. Detrick (ed.). 2002. Manual of Clinical Laboratory Immunology, 6th ed. ASM Press, Washington, D.C.
26. Szenborn, L.,, A. Tischer,, J. Pejcz,, Z. Rudkowski, and , M. Wojcik. 2003. Passive acquired immunity against measles in infants born to naturally infected and vaccinated mothers. Med. Sci. Monitor 9:CR541CR546.
27. van Binnendijk, R. S.,, S. van den Hof,, H. van den Kerkhof,, R. H. Kohl,, F. Woonink,, G. A. Berbers,, M. A. Conyn-van Spaendonck, and , T. G. Kimman. 2003. Evaluation of serological and virological tests in the diagnosis of clinical and subclinical measles virus infections during an outbreak of measles in The Netherlands. J. Infect. Dis. 188:898903.

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