Chapter 28 : Recent Developments in Rapid Detection Methods

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The continued presence of pathogenic microorganisms and their toxins in food and drinking water has necessitated the ongoing need for newer, more sensitive and robust analytical systems capable of rapid detection of these contaminants in complex samples. The ideal detection method should be capable of rapidly detecting and confirming the presence of food-borne pathogenic microorganisms directly from complex samples with no false-positive or false-negative results. Rapid detection methods including immunological detection, cell/tissue-based assays and nucleic acid-based assays have been discussed in this chapter. Conventional culture techniques continue to be the gold standard for the isolation, detection, and identification of target pathogens. These methods increase detection times by hours to days, causing preliminary test results to be delayed. These assays are defined as affinity, cell/ tissue, and nucleic acid technologies. Antibody-based detection systems are still considered to be the gold standard of affinity-based testing methods. Aptamers offer several advantages over the use of antibodies in the identification of food-borne microorganisms and toxins. Any microorganism that contains DNA or RNA can be detected using nucleic acid-based assays, but a limitation of these diagnostics is their inability to detect protein-based agents of disease, such as toxins or prions.

Citation: Goodridge L, Griffiths M. 2010. Recent Developments in Rapid Detection Methods, p 450-459. In Juneja V, Sofos J (ed), Pathogens and Toxins in Foods. ASM Press, Washington, DC. doi: 10.1128/9781555815936.ch28

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Agarose Gel Electrophoresis
DNA Microarray Analysis
Enzyme-Linked Immunosorbent Assay
Atomic Force Microscopy
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