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Chapter 10 : Hemadsorption and Hemagglutination Inhibition

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Hemadsorption and Hemagglutination Inhibition, Page 1 of 2

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Abstract:

The insertion of viral proteins necessary for budding of virus from the cell membrane facilitates the binding of red blood cells (RBCs) to the infected cells. This process is referred to as hemadsorption, and the RBCs from several species can be used in this process. A wide variety of viruses have the ability to bind with and then agglutinate RBCs (hemagglutination). The most common use of the hemagglutination inhibition (HAI) test in laboratories today is for subtyping of influenza virus isolates by state health department or World Health Organization-collaborating influenza surveillance laboratories.

Citation: Young S. 2009. Hemadsorption and Hemagglutination Inhibition, p 119-123. In Specter S, Hodinka R, Young S, Wiedbrauk D (ed), Clinical Virology Manual, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555815974.ch10
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Figures

Image of FIGURE 1
FIGURE 1

Hemadsorption. (A) Uninoculated primary monkey kidney cells; (B) primary monkey kidney cells infected with influenza A virus.

Citation: Young S. 2009. Hemadsorption and Hemagglutination Inhibition, p 119-123. In Specter S, Hodinka R, Young S, Wiedbrauk D (ed), Clinical Virology Manual, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555815974.ch10
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Image of FIGURE 2
FIGURE 2

HA endpoint determination.

Citation: Young S. 2009. Hemadsorption and Hemagglutination Inhibition, p 119-123. In Specter S, Hodinka R, Young S, Wiedbrauk D (ed), Clinical Virology Manual, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555815974.ch10
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Image of FIGURE 3
FIGURE 3

Determination of HAI titers for acute- and convalescent-phase sera.

Citation: Young S. 2009. Hemadsorption and Hemagglutination Inhibition, p 119-123. In Specter S, Hodinka R, Young S, Wiedbrauk D (ed), Clinical Virology Manual, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555815974.ch10
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Download as Powerpoint

References

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1. Hodinka, R. L. 1999. Serological tests in clinical virology, p.195–211. In E. H. Lennette and, T. F. Smith (ed.), Laboratory Diagnosis of Viral Infections, 3rd ed. Marcel Dekker, Inc., New York, NY.
2. Hsiung, G. D. 1982. Hemagglutination and the hemagglutination-inhibition test, p. 35–41. In G. D. Hsiung (ed.), Diagnostic Virology, 3rd ed. Yale University Press, New Haven, CT.
3. Leland, D. S. 1996. Hemadsorption testing, p. 195–197. In D. S. Leland (ed.), Clinical Virology. W. B. Saunders Company, Philadelphia, PA.
4. Mahony, J. B.,, and M. A. Chernesky. 1999. Immunoassay for the diagnosis of infectious diseases, p. 202–214. In P. R. Murray,, E. J. Baron,, M. A. Pfaller,, F. C. Tenover, and, R. H. Yolken (ed.), Manual of Clinical Microbiology, 7th ed. American Society for Microbiology, Washington, DC.
5. Schmidt, N. J.,, and R. W. Emmons. 1989. General principles of laboratory diagnostic methods for viral, rickettsial and chlamydial infections, p. 1–35. In N. J. Schmidt and, R. W. Emmons (ed.), Diagnostic Procedures for Viral, Rickettsial, and Chlamydial Infections, 6th ed. American Public Health Association, Washington, DC.
6. Swenson, P. D. 1992a. Detection of viruses by hemadsorption, p. 8.8.1–8.8.5. In H. D. Isenberg (ed.), Clinical Microbiology Procedures Handbook. American Society for Microbiology, Washington, DC.
7. Swenson, P. D. 1992b. Hemagglutination inhibition test for the identification of influenza viruses, p. 8.12.1–8.12.11. In H. D. Isenberg (ed.), Clinical Microbiology Procedures Handbook. American Society for Microbiology, Washington, DC.

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