1887

Chapter 15 : Restriction Analysis Challenge Worksheets

MyBook is a cheap paperback edition of the original book and will be sold at uniform, low price.

Ebook: Choose a downloadable PDF or ePub file. Chapter is a downloadable PDF file. File must be downloaded within 48 hours of purchase

Buy this Chapter
Digital (?) $30.00

Preview this chapter:
Zoom in
Zoomout

Restriction Analysis Challenge Worksheets, Page 1 of 2

| /docserver/preview/fulltext/10.1128/9781555816100/9781555814717_Chap15-1.gif /docserver/preview/fulltext/10.1128/9781555816100/9781555814717_Chap15-2.gif

Abstract:

The restriction analysis challenge worksheets contain three problems that illustrate actual uses of restriction analysis in the laboratory. The problems, particularly the second and third, require multistep analysis. They are most appropriate for advanced classes or for students who enjoy analytical puzzles. Students must be familiar with restriction enzymes, construction of recombinant DNA molecules with DNA ligase, and gel electrophoresis. The required background is found in the activities DNA Scissors, Recombinant Paper Plasmids, and DNA Goes to the Races. These worksheets require students to apply their knowledge of the construction of recombinant DNA molecules and restriction analysis to three problems like those encountered every day in research laboratories. The first two problems involve analysis of the molecules produced during a ligation, and the third requires students to generate a restriction map from restriction analysis data. In challenge I, the described ligation will produce the regenerated vector (where the ligase has simply resealed the EcoRI site in the plasmid vector), as well as the desired recombinant molecule. Challenge II is an amplification of the ideas introduced in challenge I. Here, the vector is ligated in the presence of two different inserts: the one that is to be removed and the one that is desired. Challenge III should stand on its own. There is no magic method to solve the third problem; students simply have to work with it. The chapter also provides details on restriction mapping laboratory activities, and answers to challenges.

Citation: Kreuzer H, Massey A. 2008. Restriction Analysis Challenge Worksheets, p 251-256. In Molecular Biology and Biotechnology: A Guide for Teachers, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816100.ch15

Key Concept Ranking

DNA
0.5138228
DNA Ligase
0.48627925
0.5138228
Highlighted Text: Show | Hide
Loading full text...

Full text loading...

Figures

Image of Diagram 15.1
Diagram 15.1

Challenge II. Possible products with one replication origin and a total size of less than 7,000 bp.

Citation: Kreuzer H, Massey A. 2008. Restriction Analysis Challenge Worksheets, p 251-256. In Molecular Biology and Biotechnology: A Guide for Teachers, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816100.ch15
Permissions and Reprints Request Permissions
Download as Powerpoint
Image of Diagram 15.2
Diagram 15.2

Challenge III. Restriction map of the linear DNA fragment.

Citation: Kreuzer H, Massey A. 2008. Restriction Analysis Challenge Worksheets, p 251-256. In Molecular Biology and Biotechnology: A Guide for Teachers, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816100.ch15
Permissions and Reprints Request Permissions
Download as Powerpoint
Image of Figure 15.1
Figure 15.1

Information and gel outline for restriction analysis challenge I.

Citation: Kreuzer H, Massey A. 2008. Restriction Analysis Challenge Worksheets, p 251-256. In Molecular Biology and Biotechnology: A Guide for Teachers, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816100.ch15
Permissions and Reprints Request Permissions
Download as Powerpoint
Image of Figure 15.2
Figure 15.2

Information for restriction analysis challenge II.

Citation: Kreuzer H, Massey A. 2008. Restriction Analysis Challenge Worksheets, p 251-256. In Molecular Biology and Biotechnology: A Guide for Teachers, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816100.ch15
Permissions and Reprints Request Permissions
Download as Powerpoint
Image of Figure 15.3
Figure 15.3

Gel outline for restriction analysis challenge IIC.

Citation: Kreuzer H, Massey A. 2008. Restriction Analysis Challenge Worksheets, p 251-256. In Molecular Biology and Biotechnology: A Guide for Teachers, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816100.ch15
Permissions and Reprints Request Permissions
Download as Powerpoint

References

/content/book/10.1128/9781555816100.chap15a

This is a required field
Please enter a valid email address
Please check the format of the address you have entered.
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error