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Molecular Biotechnology: Principles and Applications of Recombinant DNA, 4th Edition

Editors: Bernard R. GlickJack J. Pasternak1, Cheryl L. Patten2
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Affiliations: 1: Department of Biology, University of Waterloo Waterloo, Ontario, Canada; 2: Biology Department, University of New Brunswick Fredericton, New Brunswick, Canada;
Content Type: Textbook
Publication Year: 2010

Category: Applied and Industrial Microbiology; Best-Selling Textbook

Acclaimed by students and instructors, Molecular Biotechnology: Principles and Applications of Recombinant DNA is now in its fourth edition, bringing it thoroughly up to date with the latest findings and the latest industrial, agricultural, pharmaceutical, and biomedical applications. At the same time, the text maintains all the hallmarks that have made it a bestseller. These include its straightforward, jargon-free writing style and its extensive use of figures that help students make sense of complex biological systems and processes. These features not only enable students to grasp core concepts, but also create the foundation needed to support their own research and development work using recombinant DNA technology.

This fourth edition features greatly expanded coverage of the latest innovations in DNA sequencing techniques, therapeutics, vaccines, transgenic plants, and transgenic animals. Moreover, readers will find nearly 240 new figures to help them grasp all the latest concepts and applications.

With its broad range of topics, Molecular Biotechnology is adaptable to different upper-level undergraduate and graduate courses emphasizing particular aspects of modern biotechnology. For example, instructors can easily tailor the content to courses focusing on the fundamentals of biotechnology as well as courses dedicated to medical, agricultural, environmental, or industrial applications.

For more on the book and information on requesting an examination copy please visit http://www.asmscience.org/instructors

Book Summary

There are no separately available contributors for this publication.

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Microbiology Today

22 September 2013

I am somewhat in awe of scientists that can rise to the challenge of authoring such mighty textbooks. Leaving aside the breadth and depth of knowledge required, where did these three find the time, enthusiasm and discipline to produce nearly 1,000 pages of text with over 700 figures and tables? Furthermore, this is a no turgid tome, but a delightfully easy read that takes the reader through the many facets of molecular biotechnology - from the basics of DNA analysis and manipulation, to the ever-expanding areas in which the technology is being applied using microbes, animal and plants. For such a fast-moving field, the authors have done a tremendous job at ensuring that the 4th edition is as current as possible, and each topic is carefully and clearly explained with simple figures and key examples from the literature. Inevitably, there are some topics that have been overlooked (or perhaps kept for the even weightier volume that will be the 5th edition?). For example, there is no discussion of synthetic biology, or the use of engineered microbes in the production of biofuels - two hot areas at present. And my own pet gripe; no mention of the genetic manipulation of algae and their biotech applications. Such minor criticisms aside, this is a book that will be an invaluable core text for both undergraduate and graduate students studying biotechnology or related subjects.

Society for General Microbiology: Microbiology Today

Reviewer: Saul Purton, University College London

Review Date: October 2010

Doody Enterprises

22 September 2013

At A Glance

Acclaimed by students and instructors, "Molecular Biotechnology: Principles and Applications of Recombinant DNA" is now in its fourth edition, bringing it thoroughly up to date with the latest findings and the latest industrial, agricultural, pharmaceutical, and biomedical applications. At the same time, the text maintains all the hallmarks that have made it a bestseller. These include its straightforward, jargon-free writing style and its extensive use of figures that help students make sense of complex biological systems and processes. These features not only enable students to grasp core concepts, but also create the foundation needed to support their own research and development work using recombinant DNA technology. This fourth edition features greatly expanded coverage of the latest innovations in DNA sequencing techniques, therapeutics, vaccines, transgenic plants, and transgenic animals. Moreover, readers will find nearly 240 new figures to help them grasp all the latest concepts and applications. With its broad range of topics, Molecular Biotechnology is adaptable to different upper-level undergraduate and graduate courses emphasizing particular aspects of modern biotechnology. For example, instructors can easily tailor the content to courses focusing on the fundamentals of biotechnology as well as courses dedicated to medical, agricultural, environmental, or industrial applications. New edition presents 645 figures and 113 tables throughout the text, hundreds of which illustrate complex systems and processes. It includes chapter summaries highlighting key points. It contains references to the literature in each chapter facilitating detailed investigations of all topics covered in the text. It features end-of-chapter review questions enabling students to assess their knowledge. There are updated examples illustrating the latest concepts and applications.

Description

The fourth edition of this book, first published in 1994, details major developments in biotechnology, such as new therapeutic agents, transgenic plants, recombinant vaccines, and many more.

Purpose

The purpose is to provide a window on some of the major developments in the growing field of molecular biotechnology.

Audience

The book is intended for readers interested in molecular biotechnology. It will be very useful for students and researchers in this field.

Features

Part I of the book's four parts covers the fundamentals in eight chapters that explain the development of molecular biotechnology; DNA, RNA, and protein synthesis; recombinant DNA technology; chemical synthesis, amplification, and sequencing of DNA; bioinformatics, genomics and proteomics; manipulation of gene expression in prokaryotes; heterologous protein production in eukaryotic cells; and directed mutagenesis and protein engineering. Part II details the molecular biotechnology of microbial systems and is subdivided into nine chapters that cover, most importantly, molecular diagnostics, protein therapeutics, nucleic acids as therapeutic agents, vaccines, microbial insecticides, and large-scale production of proteins from recombinant microorganisms. The four chapters in part III on molecular biotechnology of eukaryotic systems cover methodology related to genetic engineering of plants and transgenic animals. Part IV on molecular biotechnology and society has two chapters on regulating the use of biotechnology and societal issues in biotechnology. The book is well illustrated with numerous color figures. The end-of-chapter review questions that enable the students to assess their knowledge are an important feature.

Assessment

The fourth edition captures the current concepts and developments in this growing field. Given the significant developments in molecular biotechnology, this update is absolutely justified.

Review Questionnaire-Range Question Score

1-10 Are the author's objectives met? 10

1-10 Rate the worthiness of those objectives. 10

1-5 Is this written at an appropriate level? 5

1-5 Is there significant duplication? (1=significant, 5=insignificant) 4

1-5 Are there significant omissions? (1=significant, 5=insignificant) 4

1-5 Rate the authority of the authors. 5

1-5 Are there sufficient illustrations? 5

1-5 Rate the pedagogic value of the illustrations. 5

1-5 Rate the print quality of the illustrations. 5

1-5 Are there sufficient references? 5

1-5 Rate the currency of the references. 4

1-5 Rate the pertinence of the references. 4

1-5 Rate the helpfulness of the index. 5

1-5 If important in this specialty, rate the physical appearance of the book 5

1-10 Is this a worthwhile contribution to the field? 8

1-10 If this is a 2nd or later edition, is this new edition needed? 8

Doody Enterprises

Reviewer: Omer Iqbal, MD, FACC, FESC (Loyola University Medical Center)

Review Date: 2010

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