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Chapter 23 : Genetics of Streptococcus pneumoniae

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Abstract:

The origins of genetics in can be traced to studies that began in the late 1800s with the isolation of nonencapsulated variants and ultimately led to the discovery of bacterial gene transfer by Griffith in 1928 and the identification of DNA as the genetic material by Avery, MacLeod, and McCarty in 1944. This chapter highlights much of the current information regarding genetics. Both transformation and conjugation have been described in . Transformation serves as the primary, and perhaps sole, means of transferring chromosomal genes. Conjugation occurs with plasmids that are capable of self-transfer or mobilization and with conjugative transposons that are integrated into the chromosome. can serve as both a donor and recipient in the conjugation of plasmid DNA and conjugative transposons. Generalized transduction has not been observed to occur, although a process termed pseudotransduction, which involves properties of both transduction and transformation, has been described for one pneumococcal bacteriophage. Some important similarities between many of the phages have been noted, and the complete nucleotide sequences have been determined for the genomes of phages Cp-1, Dp-1, EJ-1, and MM1. Replicating plasmids can be introduced into by electroporation. Unlike plasmids taken up via the natural transformation pathway, they are subject to restriction by the system, indicating that double-stranded DNA is transformed. The chapter discusses promoter activity of and the generation and analysis of mutants.

Citation: Yother J, Hollingshead S. 2006. Genetics of Streptococcus pneumoniae, p 275-288. In Fischetti V, Novick R, Ferretti J, Portnoy D, Rood J (ed), Gram-Positive Pathogens, Second Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816513.ch23

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Gene Expression and Regulation
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DNA Synthesis
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Two-Component Signal Transduction Systems
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Figures

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FIGURE 1

Transformation. The model is based on information discussed in the text. In some cases, the putative regulation of gene expression may be either direct or indirect. Phosphorylation of response regulator molecules by their cognate histidine kinase sensors is indicated by P. Uptake and integration of linear DNA are shown in the lower right portion, with monomer and dimer plasmid uptake shown in the central and left lower portions, respectively. Plasmid uptake will occur by the same mechanism as linear DNA uptake. For monomers, complementary overlapping strands will pair, and DNA synthesis will complete the double-stranded circular molecule. For the dimer, a small fragment of the complementary strand can serve as a primer for DNA synthesis, and circularization can occur as described in the text. Dots on the dimer molecule indicate homologous sites.

Citation: Yother J, Hollingshead S. 2006. Genetics of Streptococcus pneumoniae, p 275-288. In Fischetti V, Novick R, Ferretti J, Portnoy D, Rood J (ed), Gram-Positive Pathogens, Second Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816513.ch23
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Image of FIGURE 2
FIGURE 2

Insertion-duplication mutagenesis and restoration. (A) The effect of using an internal gene fragment to direct insertion of a nonreplicating plasmid into the chromosome. Duplication of the target fragment occurs, and the gene is disrupted by the plasmid insertion, resulting in an insertion-duplication mutation. (B) The target fragment overlaps the ends of two genes. Insertion results in duplication of the target fragment, but both genes are completely reconstructed and the result is an insertion-duplication restoration. Both genes should be functional, unless they form part of an operon, in which case the plasmid insertion would be polar on the downstream gene. The figure shows a selectable erythromycin-resistance gene () and a promoterless chloramphenicol-resistance reporter gene (), as discussed in reference .

Citation: Yother J, Hollingshead S. 2006. Genetics of Streptococcus pneumoniae, p 275-288. In Fischetti V, Novick R, Ferretti J, Portnoy D, Rood J (ed), Gram-Positive Pathogens, Second Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816513.ch23
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Tables

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TABLE 1

Derivation and properties of commonly used strains

The genotype is given, and the symbols indicate whether the strain is encapsulated (+), nonencapsulated (−), or intermediate (*). Of the type 3* strains, only Rx1 has been experimentally demonstrated to produce capsular polysaccharide ( ).

Properties are listed for strains in which they have been demonstrated, and may be the same for progenitors. Lineages and original references are described for most D39 derivatives in references and . Numbers in parentheses indicate date of original description. Except as noted, all strains are avirulent. phenotypes are from reference and the genome sequences. The 0 phenotype in Rx1 is apparently due to a mutation in the I-encoding locus. Hex phenotypes are from references and and the genome sequences. alleles are from reference or the genome sequences. All D39 derivatives are expected to carry the C1 allele.

Citation: Yother J, Hollingshead S. 2006. Genetics of Streptococcus pneumoniae, p 275-288. In Fischetti V, Novick R, Ferretti J, Portnoy D, Rood J (ed), Gram-Positive Pathogens, Second Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816513.ch23
Generic image for table
TABLE 2

Insertion sequences found in

Each element contains an open reading frame or frames with homology to transposase sequences.

Numbers in parentheses represent the number of copies in TIGR4 and the number of truncated or frameshifted copies (t).

The capsular serotypes of strains in which the element occurs; numbers indicate the fraction of tested strains containing the element.

Except as noted, information is from references and .

Alternative number of base pairs, depending on the copy.

Citation: Yother J, Hollingshead S. 2006. Genetics of Streptococcus pneumoniae, p 275-288. In Fischetti V, Novick R, Ferretti J, Portnoy D, Rood J (ed), Gram-Positive Pathogens, Second Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816513.ch23
Generic image for table
TABLE 3

Properties of some bacteriophage

Information is from citations in the text and references therein.

Activity of the N-terminal domain of the lytic enzyme.

Substrate bound by the C-terminal domain of the lytic enzyme.

The roles of the terminal proteins in these phage have not been determined.

Citation: Yother J, Hollingshead S. 2006. Genetics of Streptococcus pneumoniae, p 275-288. In Fischetti V, Novick R, Ferretti J, Portnoy D, Rood J (ed), Gram-Positive Pathogens, Second Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816513.ch23

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