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Chapter 32 : The NCTC 8325 Genome

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Abstract:

By focusing on the phage group III strain NCTC 8325, Pattee’s laboratory utilized transduction and transformation to identify a series of linkage groups that were organized into a rough genetic map. Once physical techniques for genome mapping (restriction endonuclease digestion and pulsed field gel electrophoresis) became feasible, the researcher and his coworkers attempted to fit the genetic linkage data to the physical maps of the genome. The genome sequence and annotation are now complete and are the main focus of this chapter. In addition to strain NCTC 8325, the genome sequence and annotation for at least six other strains (COL, N315, Mu50, MW2, MRSA252, MSSA476) have been completed in recent years. The complete circular genome map of NCTC 8325 shows the position of each predicted open reading frame (ORF) within the genome and the predicted functional role for each coding sequence. The current map for the NCTC 8325 genome shows the position of each of the 2,892 ORFs designated by color based on their predicted functional roles. Strain 8325 appears to contain components of at least nine potential insertion elements (IS). NCTC 8325 appears to contain fewer of the previously identified genomic islands, pathogenicity islands, or transposons than other sequenced strains.

Citation: Gillaspy A, Worrell V, Orvis J, Roe B, Dyer D, Iandolo J. 2006. The NCTC 8325 Genome, p 381-412. In Fischetti V, Novick R, Ferretti J, Portnoy D, Rood J (ed), Gram-Positive Pathogens, Second Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816513.ch32

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Mobile Genetic Elements
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Toxic Shock Syndrome
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Multilocus Sequence Typing
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Figures

Image of FIGURE 1
FIGURE 1

Genome comparison of strain NCTC 8325 and COL using the DNAMAN (Lynnon Corp.) dot-plot comparison program. Genomes were compared at the nucleotide level every 50 bp across the entire sequence. Similarity at each position is illustrated by the presence of a black dot. These two genomes are considered collinear due to the high degree of identity along the entire sequence. Numbers on the axis represent nucleotide positions in the NCTC 8325 genome while numbers on the axis represent nucleotide positions in the COL genome.

Citation: Gillaspy A, Worrell V, Orvis J, Roe B, Dyer D, Iandolo J. 2006. The NCTC 8325 Genome, p 381-412. In Fischetti V, Novick R, Ferretti J, Portnoy D, Rood J (ed), Gram-Positive Pathogens, Second Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816513.ch32
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Image of FIGURE 2
FIGURE 2

() Whole genome comparisons using the bitsum method. Coding regions from each of the six sequenced strains were used in a BLASTp analysis with ORFs from NCTC 8325. The bit scores for these BLASTp comparisons were then summed (the bitsum) and used to plot the similarity of each genome to strain 8325. Coding regions from the completed genomes for other gram-positive organisms are shown for comparison.

Citation: Gillaspy A, Worrell V, Orvis J, Roe B, Dyer D, Iandolo J. 2006. The NCTC 8325 Genome, p 381-412. In Fischetti V, Novick R, Ferretti J, Portnoy D, Rood J (ed), Gram-Positive Pathogens, Second Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816513.ch32
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Image of FIGURE 3
FIGURE 3

() Circular map of the NCTC 8325 genome. The ORFs present on the sense strand are represented on the outermost circle, the ORFs on the antisense strand are shown on the second circle, and the innermost circle is the GC skew on each coding strand. Nucleotide positions are labeled every 200,000 bp, and each of the predicted ORFs is colored based on the assigned functional role category in the final annotation. Colors and designations for each role category can be found in the legend for Fig. 4 . As is customary, the first ORF represented is .

Citation: Gillaspy A, Worrell V, Orvis J, Roe B, Dyer D, Iandolo J. 2006. The NCTC 8325 Genome, p 381-412. In Fischetti V, Novick R, Ferretti J, Portnoy D, Rood J (ed), Gram-Positive Pathogens, Second Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816513.ch32
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Image of FIGURE 4
FIGURE 4

() Graphical representation of the functional role category assignments for each of the 2,892 predicted ORFs for NCTC 8325. Numbers of genes and the percentage of the genome they represent are shown for each category. Percentage and the number of ORFs total more than 100% due to multiple role assignments for some ORFs.

Citation: Gillaspy A, Worrell V, Orvis J, Roe B, Dyer D, Iandolo J. 2006. The NCTC 8325 Genome, p 381-412. In Fischetti V, Novick R, Ferretti J, Portnoy D, Rood J (ed), Gram-Positive Pathogens, Second Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816513.ch32
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References

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26. Murphy, E.,, S. Phillips,, I. Edelman,, and R. P. Novick. 1981. Tn554: isolation and characterization of plasmid insertions. Plasmid 5:292305.
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28. Pattee, P. A., 1993. The genetic map of Staphylococcus aureus, p. 489496. In A. L. Sonenshein,, J. A. Hoch,, and R. Losick (ed.), Bacillus subtilis and Other Gram-Positive Bacteria: Biochemistry, Physiology, and Molecular Genetics. American Society for Microbiology, Washington, D.C.
29. Rouch, D. A.,, L. J. Messerotti,, L. S. Loo,, C. A. Jackson,, and R. A. Skurray. 1989. Trimethoprim resistance transposon Tn4003 from Staphylococcus aureus encodes genes for a dihydrofolate reductase and thymidylate synthetase flanked by three copies of IS257. Mol. Microbiol. 3:161175.
30. Rowland, S. J.,, and K. G. Dyke. 1989. Characterization of the staphylococcal beta-lactamase transposon Tn552. EMBO J. 8:27612773.
31. Ruzin, A.,, J. Lindsay,, and R. P. Novick. 2001. Molecular genetics of SaPI1—a mobile pathogenicity island in Staphylococcus aureus. Mol. Microbiol. 41:365377.
33. Townsend, D. E.,, S. Bolton,, N. Ashdown,, D. I. Annear,, and W. B. Grubb. 1986. Conjugative, staphylococcal plasmids carrying hitch-hiking transposons similar to Tn554: intra- and interspecies dissemination of erythromycin resistance. Aust. J. Exp. Biol. Med. Sci. 64:367379.
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Tables

Generic image for table
TABLE 1a

Tabular listing of all predicted ORFs in NCTC 8325 listed by functional role category based on TIGR roles

Note that a single ORF may be listed in more than one role category.

Gene designations are based on those previously defined for or , if available. Duplicate gene names may occur as a result of multiple alleles encoding homologous genes or unresolved frameshifts. For lack of space, genes designated as hypothetical or conserved hypothetical are not listed. A complete listing is available on our website (http://microgen.ouhsc.edu).

Citation: Gillaspy A, Worrell V, Orvis J, Roe B, Dyer D, Iandolo J. 2006. The NCTC 8325 Genome, p 381-412. In Fischetti V, Novick R, Ferretti J, Portnoy D, Rood J (ed), Gram-Positive Pathogens, Second Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816513.ch32
Generic image for table
TABLE 1b

Tabular listing of all predicted ORFs in NCTC 8325 listed by functional role category based on TIGR roles

Note that a single ORF may be listed in more than one role category.

Gene designations are based on those previously defined for or , if available. Duplicate gene names may occur as a result of multiple alleles encoding homologous genes or unresolved frameshifts. For lack of space, genes designated as hypothetical or conserved hypothetical are not listed. A complete listing is available on our website (http://microgen.ouhsc.edu).

Citation: Gillaspy A, Worrell V, Orvis J, Roe B, Dyer D, Iandolo J. 2006. The NCTC 8325 Genome, p 381-412. In Fischetti V, Novick R, Ferretti J, Portnoy D, Rood J (ed), Gram-Positive Pathogens, Second Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816513.ch32
Generic image for table
TABLE 1c

Tabular listing of all predicted ORFs in NCTC 8325 listed by functional role category based on TIGR roles

Note that a single ORF may be listed in more than one role category.

Gene designations are based on those previously defined for or , if available. Duplicate gene names may occur as a result of multiple alleles encoding homologous genes or unresolved frameshifts. For lack of space, genes designated as hypothetical or conserved hypothetical are not listed. A complete listing is available on our website (http://microgen.ouhsc.edu).

Citation: Gillaspy A, Worrell V, Orvis J, Roe B, Dyer D, Iandolo J. 2006. The NCTC 8325 Genome, p 381-412. In Fischetti V, Novick R, Ferretti J, Portnoy D, Rood J (ed), Gram-Positive Pathogens, Second Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816513.ch32
Generic image for table
TABLE 1d

Tabular listing of all predicted ORFs in NCTC 8325 listed by functional role category based on TIGR roles

Note that a single ORF may be listed in more than one role category.

Gene designations are based on those previously defined for or , if available. Duplicate gene names may occur as a result of multiple alleles encoding homologous genes or unresolved frameshifts. For lack of space, genes designated as hypothetical or conserved hypothetical are not listed. A complete listing is available on our website (http://microgen.ouhsc.edu).

Citation: Gillaspy A, Worrell V, Orvis J, Roe B, Dyer D, Iandolo J. 2006. The NCTC 8325 Genome, p 381-412. In Fischetti V, Novick R, Ferretti J, Portnoy D, Rood J (ed), Gram-Positive Pathogens, Second Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816513.ch32
Generic image for table
TABLE 1e

Tabular listing of all predicted ORFs in NCTC 8325 listed by functional role category based on TIGR roles

Note that a single ORF may be listed in more than one role category.

Gene designations are based on those previously defined for or , if available. Duplicate gene names may occur as a result of multiple alleles encoding homologous genes or unresolved frameshifts. For lack of space, genes designated as hypothetical or conserved hypothetical are not listed. A complete listing is available on our website (http://microgen.ouhsc.edu).

Citation: Gillaspy A, Worrell V, Orvis J, Roe B, Dyer D, Iandolo J. 2006. The NCTC 8325 Genome, p 381-412. In Fischetti V, Novick R, Ferretti J, Portnoy D, Rood J (ed), Gram-Positive Pathogens, Second Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816513.ch32
Generic image for table
TABLE 1f

Tabular listing of all predicted ORFs in NCTC 8325 listed by functional role category based on TIGR roles

Note that a single ORF may be listed in more than one role category.

Gene designations are based on those previously defined for or , if available. Duplicate gene names may occur as a result of multiple alleles encoding homologous genes or unresolved frameshifts. For lack of space, genes designated as hypothetical or conserved hypothetical are not listed. A complete listing is available on our website (http://microgen.ouhsc.edu).

Citation: Gillaspy A, Worrell V, Orvis J, Roe B, Dyer D, Iandolo J. 2006. The NCTC 8325 Genome, p 381-412. In Fischetti V, Novick R, Ferretti J, Portnoy D, Rood J (ed), Gram-Positive Pathogens, Second Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816513.ch32
Generic image for table
TABLE 1g

Tabular listing of all predicted ORFs in NCTC 8325 listed by functional role category based on TIGR roles

Note that a single ORF may be listed in more than one role category.

Gene designations are based on those previously defined for or , if available. Duplicate gene names may occur as a result of multiple alleles encoding homologous genes or unresolved frameshifts. For lack of space, genes designated as hypothetical or conserved hypothetical are not listed. A complete listing is available on our website (http://microgen.ouhsc.edu).

Citation: Gillaspy A, Worrell V, Orvis J, Roe B, Dyer D, Iandolo J. 2006. The NCTC 8325 Genome, p 381-412. In Fischetti V, Novick R, Ferretti J, Portnoy D, Rood J (ed), Gram-Positive Pathogens, Second Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816513.ch32
Generic image for table
TABLE 1h

Tabular listing of all predicted ORFs in NCTC 8325 listed by functional role category based on TIGR roles

Note that a single ORF may be listed in more than one role category.

Gene designations are based on those previously defined for or , if available. Duplicate gene names may occur as a result of multiple alleles encoding homologous genes or unresolved frameshifts. For lack of space, genes designated as hypothetical or conserved hypothetical are not listed. A complete listing is available on our website (http://microgen.ouhsc.edu).

Citation: Gillaspy A, Worrell V, Orvis J, Roe B, Dyer D, Iandolo J. 2006. The NCTC 8325 Genome, p 381-412. In Fischetti V, Novick R, Ferretti J, Portnoy D, Rood J (ed), Gram-Positive Pathogens, Second Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816513.ch32
Generic image for table
TABLE 1i

Tabular listing of all predicted ORFs in NCTC 8325 listed by functional role category based on TIGR roles

Note that a single ORF may be listed in more than one role category.

Gene designations are based on those previously defined for or , if available. Duplicate gene names may occur as a result of multiple alleles encoding homologous genes or unresolved frameshifts. For lack of space, genes designated as hypothetical or conserved hypothetical are not listed. A complete listing is available on our website (http://microgen.ouhsc.edu).

Citation: Gillaspy A, Worrell V, Orvis J, Roe B, Dyer D, Iandolo J. 2006. The NCTC 8325 Genome, p 381-412. In Fischetti V, Novick R, Ferretti J, Portnoy D, Rood J (ed), Gram-Positive Pathogens, Second Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816513.ch32
Generic image for table
TABLE 1j

Tabular listing of all predicted ORFs in NCTC 8325 listed by functional role category based on TIGR roles

Note that a single ORF may be listed in more than one role category.

Gene designations are based on those previously defined for or , if available. Duplicate gene names may occur as a result of multiple alleles encoding homologous genes or unresolved frameshifts. For lack of space, genes designated as hypothetical or conserved hypothetical are not listed. A complete listing is available on our website (http://microgen.ouhsc.edu).

Citation: Gillaspy A, Worrell V, Orvis J, Roe B, Dyer D, Iandolo J. 2006. The NCTC 8325 Genome, p 381-412. In Fischetti V, Novick R, Ferretti J, Portnoy D, Rood J (ed), Gram-Positive Pathogens, Second Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816513.ch32
Generic image for table
TABLE 1k

Tabular listing of all predicted ORFs in NCTC 8325 listed by functional role category based on TIGR roles

Note that a single ORF may be listed in more than one role category.

Gene designations are based on those previously defined for or , if available. Duplicate gene names may occur as a result of multiple alleles encoding homologous genes or unresolved frameshifts. For lack of space, genes designated as hypothetical or conserved hypothetical are not listed. A complete listing is available on our website (http://microgen.ouhsc.edu).

Citation: Gillaspy A, Worrell V, Orvis J, Roe B, Dyer D, Iandolo J. 2006. The NCTC 8325 Genome, p 381-412. In Fischetti V, Novick R, Ferretti J, Portnoy D, Rood J (ed), Gram-Positive Pathogens, Second Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816513.ch32
Generic image for table
TABLE 1l

Tabular listing of all predicted ORFs in NCTC 8325 listed by functional role category based on TIGR roles

Note that a single ORF may be listed in more than one role category.

Gene designations are based on those previously defined for or , if available. Duplicate gene names may occur as a result of multiple alleles encoding homologous genes or unresolved frameshifts. For lack of space, genes designated as hypothetical or conserved hypothetical are not listed. A complete listing is available on our website (http://microgen.ouhsc.edu).

Citation: Gillaspy A, Worrell V, Orvis J, Roe B, Dyer D, Iandolo J. 2006. The NCTC 8325 Genome, p 381-412. In Fischetti V, Novick R, Ferretti J, Portnoy D, Rood J (ed), Gram-Positive Pathogens, Second Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816513.ch32
Generic image for table
TABLE 1m

Tabular listing of all predicted ORFs in NCTC 8325 listed by functional role category based on TIGR roles

Note that a single ORF may be listed in more than one role category.

Gene designations are based on those previously defined for or , if available. Duplicate gene names may occur as a result of multiple alleles encoding homologous genes or unresolved frameshifts. For lack of space, genes designated as hypothetical or conserved hypothetical are not listed. A complete listing is available on our website (http://microgen.ouhsc.edu).

Citation: Gillaspy A, Worrell V, Orvis J, Roe B, Dyer D, Iandolo J. 2006. The NCTC 8325 Genome, p 381-412. In Fischetti V, Novick R, Ferretti J, Portnoy D, Rood J (ed), Gram-Positive Pathogens, Second Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816513.ch32
Generic image for table
TABLE 1n

Tabular listing of all predicted ORFs in NCTC 8325 listed by functional role category based on TIGR roles

Note that a single ORF may be listed in more than one role category.

Gene designations are based on those previously defined for or , if available. Duplicate gene names may occur as a result of multiple alleles encoding homologous genes or unresolved frameshifts. For lack of space, genes designated as hypothetical or conserved hypothetical are not listed. A complete listing is available on our website (http://microgen.ouhsc.edu).

Citation: Gillaspy A, Worrell V, Orvis J, Roe B, Dyer D, Iandolo J. 2006. The NCTC 8325 Genome, p 381-412. In Fischetti V, Novick R, Ferretti J, Portnoy D, Rood J (ed), Gram-Positive Pathogens, Second Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816513.ch32
Generic image for table
TABLE 1

Tabular listing of all predicted ORFs in NCTC 8325 listed by functional role category based on TIGR roles

Note that a single ORF may be listed in more than one role category.

Gene designations are based on those previously defined for or , if available. Duplicate gene names may occur as a result of multiple alleles encoding homologous genes or unresolved frameshifts. For lack of space, genes designated as hypothetical or conserved hypothetical are not listed. A complete listing is available on our website (http://microgen.ouhsc.edu).

Citation: Gillaspy A, Worrell V, Orvis J, Roe B, Dyer D, Iandolo J. 2006. The NCTC 8325 Genome, p 381-412. In Fischetti V, Novick R, Ferretti J, Portnoy D, Rood J (ed), Gram-Positive Pathogens, Second Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816513.ch32
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TABLE 1p

Tabular listing of all predicted ORFs in NCTC 8325 listed by functional role category based on TIGR roles

Note that a single ORF may be listed in more than one role category.

Gene designations are based on those previously defined for or , if available. Duplicate gene names may occur as a result of multiple alleles encoding homologous genes or unresolved frameshifts. For lack of space, genes designated as hypothetical or conserved hypothetical are not listed. A complete listing is available on our website (http://microgen.ouhsc.edu).

Citation: Gillaspy A, Worrell V, Orvis J, Roe B, Dyer D, Iandolo J. 2006. The NCTC 8325 Genome, p 381-412. In Fischetti V, Novick R, Ferretti J, Portnoy D, Rood J (ed), Gram-Positive Pathogens, Second Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816513.ch32
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TABLE 1q

Tabular listing of all predicted ORFs in NCTC 8325 listed by functional role category based on TIGR roles

Note that a single ORF may be listed in more than one role category.

Gene designations are based on those previously defined for or , if available. Duplicate gene names may occur as a result of multiple alleles encoding homologous genes or unresolved frameshifts. For lack of space, genes designated as hypothetical or conserved hypothetical are not listed. A complete listing is available on our website (http://microgen.ouhsc.edu).

Citation: Gillaspy A, Worrell V, Orvis J, Roe B, Dyer D, Iandolo J. 2006. The NCTC 8325 Genome, p 381-412. In Fischetti V, Novick R, Ferretti J, Portnoy D, Rood J (ed), Gram-Positive Pathogens, Second Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816513.ch32
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TABLE 1r

Tabular listing of all predicted ORFs in NCTC 8325 listed by functional role category based on TIGR roles

Note that a single ORF may be listed in more than one role category.

Gene designations are based on those previously defined for or , if available. Duplicate gene names may occur as a result of multiple alleles encoding homologous genes or unresolved frameshifts. For lack of space, genes designated as hypothetical or conserved hypothetical are not listed. A complete listing is available on our website (http://microgen.ouhsc.edu).

Citation: Gillaspy A, Worrell V, Orvis J, Roe B, Dyer D, Iandolo J. 2006. The NCTC 8325 Genome, p 381-412. In Fischetti V, Novick R, Ferretti J, Portnoy D, Rood J (ed), Gram-Positive Pathogens, Second Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816513.ch32
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TABLE 1s

Tabular listing of all predicted ORFs in NCTC 8325 listed by functional role category based on TIGR roles

Note that a single ORF may be listed in more than one role category.

Gene designations are based on those previously defined for or , if available. Duplicate gene names may occur as a result of multiple alleles encoding homologous genes or unresolved frameshifts. For lack of space, genes designated as hypothetical or conserved hypothetical are not listed. A complete listing is available on our website (http://microgen.ouhsc.edu).

Citation: Gillaspy A, Worrell V, Orvis J, Roe B, Dyer D, Iandolo J. 2006. The NCTC 8325 Genome, p 381-412. In Fischetti V, Novick R, Ferretti J, Portnoy D, Rood J (ed), Gram-Positive Pathogens, Second Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816513.ch32
Generic image for table
TABLE 1t

Tabular listing of all predicted ORFs in NCTC 8325 listed by functional role category based on TIGR roles

Note that a single ORF may be listed in more than one role category.

Gene designations are based on those previously defined for or , if available. Duplicate gene names may occur as a result of multiple alleles encoding homologous genes or unresolved frameshifts. For lack of space, genes designated as hypothetical or conserved hypothetical are not listed. A complete listing is available on our website (http://microgen.ouhsc.edu).

Citation: Gillaspy A, Worrell V, Orvis J, Roe B, Dyer D, Iandolo J. 2006. The NCTC 8325 Genome, p 381-412. In Fischetti V, Novick R, Ferretti J, Portnoy D, Rood J (ed), Gram-Positive Pathogens, Second Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816513.ch32
Generic image for table
TABLE 1u

Tabular listing of all predicted ORFs in NCTC 8325 listed by functional role category based on TIGR roles

Note that a single ORF may be listed in more than one role category.

Gene designations are based on those previously defined for or , if available. Duplicate gene names may occur as a result of multiple alleles encoding homologous genes or unresolved frameshifts. For lack of space, genes designated as hypothetical or conserved hypothetical are not listed. A complete listing is available on our website (http://microgen.ouhsc.edu).

Citation: Gillaspy A, Worrell V, Orvis J, Roe B, Dyer D, Iandolo J. 2006. The NCTC 8325 Genome, p 381-412. In Fischetti V, Novick R, Ferretti J, Portnoy D, Rood J (ed), Gram-Positive Pathogens, Second Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816513.ch32
Generic image for table
TABLE 1

Tabular listing of all predicted ORFs in NCTC 8325 listed by functional role category based on TIGR roles

Note that a single ORF may be listed in more than one role category.

Gene designations are based on those previously defined for or , if available. Duplicate gene names may occur as a result of multiple alleles encoding homologous genes or unresolved frameshifts. For lack of space, genes designated as hypothetical or conserved hypothetical are not listed. A complete listing is available on our website (http://microgen.ouhsc.edu).

Citation: Gillaspy A, Worrell V, Orvis J, Roe B, Dyer D, Iandolo J. 2006. The NCTC 8325 Genome, p 381-412. In Fischetti V, Novick R, Ferretti J, Portnoy D, Rood J (ed), Gram-Positive Pathogens, Second Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816513.ch32
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TABLE 1w

Tabular listing of all predicted ORFs in NCTC 8325 listed by functional role category based on TIGR roles

Note that a single ORF may be listed in more than one role category.

Gene designations are based on those previously defined for or , if available. Duplicate gene names may occur as a result of multiple alleles encoding homologous genes or unresolved frameshifts. For lack of space, genes designated as hypothetical or conserved hypothetical are not listed. A complete listing is available on our website (http://microgen.ouhsc.edu).

Citation: Gillaspy A, Worrell V, Orvis J, Roe B, Dyer D, Iandolo J. 2006. The NCTC 8325 Genome, p 381-412. In Fischetti V, Novick R, Ferretti J, Portnoy D, Rood J (ed), Gram-Positive Pathogens, Second Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816513.ch32
Generic image for table
TABLE 1x

Tabular listing of all predicted ORFs in NCTC 8325 listed by functional role category based on TIGR roles

Note that a single ORF may be listed in more than one role category.

Gene designations are based on those previously defined for or , if available. Duplicate gene names may occur as a result of multiple alleles encoding homologous genes or unresolved frameshifts. For lack of space, genes designated as hypothetical or conserved hypothetical are not listed. A complete listing is available on our website (http://microgen.ouhsc.edu).

Citation: Gillaspy A, Worrell V, Orvis J, Roe B, Dyer D, Iandolo J. 2006. The NCTC 8325 Genome, p 381-412. In Fischetti V, Novick R, Ferretti J, Portnoy D, Rood J (ed), Gram-Positive Pathogens, Second Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816513.ch32
Generic image for table
TABLE 1y

Tabular listing of all predicted ORFs in NCTC 8325 listed by functional role category based on TIGR roles

Note that a single ORF may be listed in more than one role category.

Gene designations are based on those previously defined for or , if available. Duplicate gene names may occur as a result of multiple alleles encoding homologous genes or unresolved frameshifts. For lack of space, genes designated as hypothetical or conserved hypothetical are not listed. A complete listing is available on our website (http://microgen.ouhsc.edu).

Citation: Gillaspy A, Worrell V, Orvis J, Roe B, Dyer D, Iandolo J. 2006. The NCTC 8325 Genome, p 381-412. In Fischetti V, Novick R, Ferretti J, Portnoy D, Rood J (ed), Gram-Positive Pathogens, Second Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816513.ch32
Generic image for table
TABLE 1z

Tabular listing of all predicted ORFs in NCTC 8325 listed by functional role category based on TIGR roles

Note that a single ORF may be listed in more than one role category.

Gene designations are based on those previously defined for or , if available. Duplicate gene names may occur as a result of multiple alleles encoding homologous genes or unresolved frameshifts. For lack of space, genes designated as hypothetical or conserved hypothetical are not listed. A complete listing is available on our website (http://microgen.ouhsc.edu).

Citation: Gillaspy A, Worrell V, Orvis J, Roe B, Dyer D, Iandolo J. 2006. The NCTC 8325 Genome, p 381-412. In Fischetti V, Novick R, Ferretti J, Portnoy D, Rood J (ed), Gram-Positive Pathogens, Second Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816513.ch32
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TABLE 2

The SaPl family

Citation: Gillaspy A, Worrell V, Orvis J, Roe B, Dyer D, Iandolo J. 2006. The NCTC 8325 Genome, p 381-412. In Fischetti V, Novick R, Ferretti J, Portnoy D, Rood J (ed), Gram-Positive Pathogens, Second Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816513.ch32

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